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Search for saliva biomarkers used for mental health management of junior high school student

Research Project

Project/Area Number 19500473
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Rehabilitation science/Welfare engineering
Research InstitutionIwate University (2008-2009)
University of Toyama (2007)

Principal Investigator

YAMAGUCHI Masaki  Iwate University, 工学部, 教授 (50272638)

Co-Investigator(Kenkyū-buntansha) TAKEDA Kazunori  筑波大学, 大学院・人間総合科学研究科, 教授 (90261768)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsバイオマーカー / モニタリング / 唾液 / 児童生徒 / ストレス / 情動 / 交感神経 / センサ
Research Abstract

In order to search for salivary biomarkers used for the mental health management of junior high school student, a psychosomatic stress test, a search for salivary biomarkers and an improvement on the psychology education were carried out. It was revealed that the salivary amylase activity (sAA) statistically correlate with the subjective evaluation of psychosomatic stress from a salivary test using seventy healthy subjects in first-year junior high school students for 3 weeks. Three salivary stress markers, sAA as an index of sympathetic nervous system, salivary cortisol (sCORT) as an index of endocrine system and salivary IgA (sIgA) as an index of immune system, were identified through the psychosomatic stress test. It was indicated that the salivary stress markers might be a useful screening method for preventing to be a non-attendance at school using the multiple regression analysis.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (56 results)

All 2010 2009 2008 2007 Other

All Journal Article (20 results) (of which Peer Reviewed: 10 results) Presentation (20 results) Book (4 results) Remarks (3 results) Patent(Industrial Property Rights) (9 results)

  • [Journal Article] Immunochromatographic cortisol biosensor using enzyme-labeled conjugate2010

    • Author(s)
      Yusuke Tahara, Suguru Yoshikawa, Vivek Shetty, Masaki Yamaguchi
    • Journal Title

      Sensors and Materials Vol.22(in press)

    • Related Report
      2009 Final Research Report
  • [Journal Article] Immunochromatographic cortisol biosensor using enzyme-labeled conjugate2010

    • Author(s)
      山口昌樹
    • Journal Title

      Sensors and Materials Vol.22(in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Influence of concentration of fragrances on salivary α-amylase2009

    • Author(s)
      M. Yamaguchi, Y. Tahara, S. Kosaka
    • Journal Title

      International Journal of Cosmetic Science Vol.31, No.5

      Pages: 391-395

    • Related Report
      2009 Final Research Report
  • [Journal Article] 唾液アミラーゼモニターの検査成績2009

    • Author(s)
      山口昌樹, 吉田博, 岡部則夫
    • Journal Title

      ライフサポート Vol.21, No.3

      Pages: 130-134

    • NAID

      10029238047

    • Related Report
      2009 Final Research Report
  • [Journal Article] 唾液アミラーゼ活性は中学生の心身ストレスの指標になり得るか2009

    • Author(s)
      村上満, 田原祐助, 竹田 一則, 山口昌樹
    • Journal Title

      生体医工学 Vol.47, No.2

      Pages: 166-171

    • NAID

      10025974203

    • Related Report
      2009 Final Research Report
  • [Journal Article] Evaluation of environmental and physiological factors of a whole ceiling-type air conditioner using a salivary biomarker2009

    • Author(s)
      Yusuke Tahara, Naomi Morito, Hajime Nishimiya, Hideyuki Yamagishi, Masaki Yamaguchi
    • Journal Title

      Building & Environment Vol.44, No.6

      Pages: 1156-1161

    • Related Report
      2009 Final Research Report
  • [Journal Article] 唾液アミラーゼ活性は中学生の心身ストレスの指標になり得るか2009

    • Author(s)
      山口昌樹
    • Journal Title

      生体医工学 Vol.47

      Pages: 166-171

    • NAID

      10025974203

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 唾液アミラーゼモニターの検査成績2009

    • Author(s)
      山口昌樹
    • Journal Title

      ライフサポート Vol.21

      Pages: 130-134

    • NAID

      10029238047

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Influence of concentration of fragrances on salivary α-amylase2009

    • Author(s)
      山口昌樹
    • Journal Title

      International Journal of Cosmetic Science Vol.31

      Pages: 391-395

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of environmental and physiological factors of a whole ceiling-type air conditioner using a salivary biomarker2009

    • Author(s)
      Yusuke Tahara
    • Journal Title

      Building & Environment Vol.44

      Pages: 1156-1161

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Method for Evaluating the Discomfort Induced by Odor Using a Biochemical Marker2008

    • Author(s)
      Masaki Yamaguchi, Josaku Sakakima, Shoshi Kosaka, Minako Nakabayashi
    • Journal Title

      Sensors & Actuators B 131

      Pages: 143-147

    • Related Report
      2009 Final Research Report
  • [Journal Article] Correlation of Salivary Amylase Activity With Eustress in Patients With Severe Motor and Intellectual Disabilities2008

    • Author(s)
      Kazunori TAKEDA, Maiko WATANABE, Mieko ONISHI, Masaki YAMAGUCHI
    • Journal Title

      The Japanese Journal of Special Education Vol.45, No.6

      Pages: 447-457

    • NAID

      110007329169

    • Related Report
      2009 Final Research Report
  • [Journal Article] Competitive and product inhibition-based a-amylase activity analysis method2008

    • Author(s)
      Masaki Yamaguchi, Junichi Wakasugi, Josaku Sakakima
    • Journal Title

      Clinical Biochemistry Vol.41, No.4-5

      Pages: 325-330

    • Related Report
      2009 Final Research Report
  • [Journal Article] Correlation of Salivary Amylase Activity With Eustress in Patients With Severe Motor and Intellectual Disabilities2008

    • Author(s)
      Kazunori TAKEDA
    • Journal Title

      The Japanese Journal of Special Education Vol.45

      Pages: 447-457

    • NAID

      110007329169

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Method for Evaluating the Discomfort Induced by Odor Using a Biochemical Marker2008

    • Author(s)
      Masaki Yamaguchi
    • Journal Title

      Sensors & Actuators B 131

      Pages: 143-147

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Competitive and product inhibition-based α-amylase activity analysismethod2008

    • Author(s)
      Masaki Yamaguchi
    • Journal Title

      Clinical Biochemistry Vol. 41

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of the acute sedative effect of fragrances based on a biochemical marker2007

    • Author(s)
      Masaki Yamaguchi, Naoko Hanawa, Kei Hamazaki, Kimiharu Sato, Kimihiro Nakano
    • Journal Title

      The Journal of Essential Oil Research Vol.19, No.5

      Pages: 470-476

    • Related Report
      2009 Final Research Report
  • [Journal Article] 唾液アミラーゼ式交感神経モニタの基礎的性能2007

    • Author(s)
      山口昌樹, 花輪尚子, 吉田博
    • Journal Title

      生体医工学 Vol.45, No.2

      Pages: 161-168

    • NAID

      110006402720

    • Related Report
      2009 Final Research Report
  • [Journal Article] 唾液アミラーゼ式交感神経モニタの基礎的性能2007

    • Author(s)
      山口 昌樹
    • Journal Title

      生体医工学 Vol. 45

      Pages: 161-168

    • NAID

      110006402720

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of the acute sedative effect of fragrances based on a biochemical marker2007

    • Author(s)
      Masaki Yamaguchi
    • Journal Title

      The Journal of Essential Oil Research Vol. 19

      Pages: 470-476

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 有限要素法を用いたイムノセンサのデジタルシミュレーションへのアプローチ2010

    • Author(s)
      山口昌樹, 高橋貴之, 吉野雄一朗, 佐々木誠
    • Organizer
      電気化学会第77回大会
    • Place of Presentation
      富山大学
    • Year and Date
      2010-03-31
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] Point-of-Use Measurement of Salivary Cortisol levels2009

    • Author(s)
      Masaki Yamaguchi, Suguru Yoshikawa, Yusuke Tahara, Daisuke Niwa, Yoshikatsu Imai, Vivek Shetty
    • Organizer
      The 8th IEEE Conference on Sensors (Sensors2009)
    • Place of Presentation
      Christchurch, New Zealand
    • Year and Date
      2009-10-26
    • Related Report
      2009 Final Research Report
  • [Presentation] Point-of-Use Measurement of Salivary Cortisol levels2009

    • Author(s)
      山口昌樹
    • Organizer
      The 8th IEEE Conference on Sensors
    • Place of Presentation
      クライストチャーチコンベンションセンター(New Zealand)
    • Year and Date
      2009-10-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] ストレスを評価する2009

    • Author(s)
      山口昌樹
    • Organizer
      第46回日本臨床生理学会総会 教育講演
    • Place of Presentation
      岩手県民情報交流センター
    • Year and Date
      2009-10-23
    • Related Report
      2009 Final Research Report
  • [Presentation] ストレスを評価する(教育講演)2009

    • Author(s)
      山口昌樹
    • Organizer
      第46回日本臨床生理学会総会
    • Place of Presentation
      岩手県民情報センター(盛岡)
    • Year and Date
      2009-10-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] Diurnal variation of salivary cortisol and amylase activity in fatigued state2009

    • Author(s)
      Y. Nakajima, T. Takahashi, M. Yamaguchi
    • Organizer
      Environmental Health Risk 2009, WIT Transactions on Biomedicine and Health
    • Place of Presentation
      Ashurst, Southampton, UK
    • Year and Date
      2009-09-21
    • Related Report
      2009 Final Research Report
  • [Presentation] Diurnal variation of salivary cortisol and amylase activity in fatigued state2009

    • Author(s)
      山口昌樹
    • Organizer
      Environmental Health Risk 2009
    • Place of Presentation
      Wessex Institute of Technology(UK)
    • Year and Date
      2009-09-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] Immuno-chromatographic Test-strip for Skin Cathepsin L Analysis2009

    • Author(s)
      M. Yamaguchi
    • Organizer
      Pacific Rim Meeting on Electrochemical and Solid-state Science (PRiME2008)
    • Place of Presentation
      Honolulu, HI
    • Year and Date
      2009-09-14
    • Related Report
      2008 Annual Research Report
  • [Presentation] Mental Health Evaluation using Chemical Sensor as Human-Machine Interface2009

    • Author(s)
      Yusuke Tahara, Takayuki Takahashi, Kazunori Takeda, Tasuku Miyoshi, Masaki Yamaguchi
    • Organizer
      2009 IEEE 11th International Conference on Rehabilitation Robotics (ICORR2009) WeEPOD3.4
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2009-06-25
    • Related Report
      2009 Final Research Report
  • [Presentation] Mental Health Evaluation using Chemical Sensor as Human-Machine Interface2009

    • Author(s)
      山口昌樹
    • Organizer
      2009 IEEE 11th International Conference on Rehabilitation Robotics(ICORR2009)
    • Place of Presentation
      京都国際会館
    • Year and Date
      2009-06-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] データマイニングを用いた唾液バイオマーカーに基づく中学生の不健康群の推定2009

    • Author(s)
      山口昌樹, 高橋貴之, 田原祐助, 三好扶, 吉田博, 竹田一則
    • Organizer
      日本生体医工学会大会プログラム・論文集
    • Place of Presentation
      東京都タワーホール船堀
    • Year and Date
      2009-04-23
    • Related Report
      2009 Final Research Report
  • [Presentation] データマイニングを用いた唾液バイオマーカーに基づく中学生の不健康群の推定2009

    • Author(s)
      山口昌樹
    • Organizer
      第48回日本生体医工学会大会
    • Place of Presentation
      クリスタルホール船堀(東京)
    • Year and Date
      2009-04-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] Noninvasively Evaluation on Human Stress Using Salivary Biomarker2008

    • Author(s)
      Yamaguchi M
    • Organizer
      EHRLICH II-2nd World Conference on Magic Bullets
    • Place of Presentation
      Nurnberg, Germany
    • Year and Date
      2008-10-03
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] Immuno-chromatographic Test-strip for Skin Cathepsin L Analysis2008

    • Author(s)
      M. Yamaguchi, S. Kosaka, Y. Tahara, A. Date
    • Organizer
      Pacific Rim Meeting on Electrochemical and Solid-state Science (PRiME2008), ECS Transactions : Chemical Sensors 8
    • Place of Presentation
      Honolulu, HI, USA
    • Year and Date
      2008-09-14
    • Related Report
      2009 Final Research Report
  • [Presentation] Synthesis of a Glucose Oxidase-Cortisol Conjugate Used for Electrochemical Biosensor for Cortisol Analysis2008

    • Author(s)
      Masaki YAMAGUCHI, Yusuke TAHARA, Shoshi KOSAKA, Vivek SHETTY
    • Organizer
      The International Conference on Electrical Engineering 2008 (ICEE2008)
    • Place of Presentation
      Okinawa, Japan
    • Year and Date
      2008-07-09
    • Related Report
      2009 Final Research Report
  • [Presentation] Synthesis of a Glucose Oxidase-Cortisol Conjugate Used for Electrochemical Biosensor for Cortisol Analysis2008

    • Author(s)
      Masaki YAMAGUCHI
    • Organizer
      The International Conference on Electrical Engineering 2008(ICEE2008)
    • Place of Presentation
      Okinawa, Japan
    • Year and Date
      2008-07-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] A NOVEL HAND-HELD MONITOR OF SYMPATHETIC NERVOUS SYSTEM USING BIOMARKER2007

    • Author(s)
      Masaki Yamaguchi, Kazutaka Teramura, Yusuke Tahara, Josaku Sakakima
    • Organizer
      2nd World Conference of Stress (STRESS07), Book of Abstracts
    • Place of Presentation
      Budapest, Hungary
    • Year and Date
      2007-08-24
    • Related Report
      2009 Final Research Report
  • [Presentation] ANOVEL HAND-HELD MONITOR OF SYMPATHETIC NERVOUS SYSTEM USING BIOMARKER2007

    • Author(s)
      Masaki Yamaguchi
    • Organizer
      2nd World Conference of Stress(STRESSO7)
    • Place of Presentation
      Budapest, Hungary
    • Year and Date
      2007-08-24
    • Related Report
      2007 Annual Research Report
  • [Presentation] Evaluation Device of Discomfort Effect of Odor Using Biochemical Marker2007

    • Author(s)
      M. Yamaguchi, J. Sakakima, M. Nakabayashi, S. Kosaka
    • Organizer
      International Symposium on Olfaction and Electronic Nose (ISOEN 2007)
    • Place of Presentation
      Petersburg, Russia
    • Year and Date
      2007-05-05
    • Related Report
      2009 Final Research Report
  • [Presentation] Evaluation Device of Discomfort Effect of Odor Using Biochemical Marker2007

    • Author(s)
      M. Yaxnaguchi
    • Organizer
      Intemational Symposium Olfaction and Electronic Nose(ISOEN 2007)
    • Place of Presentation
      Petersburg, Russia
    • Year and Date
      2007-05-05
    • Related Report
      2007 Annual Research Report
  • [Book] ヒューマンサイエンスとセンシング調査研究委員会編, 心と体のセンシング2009

    • Author(s)
      山口昌樹(分担執筆)
    • Total Pages
      180
    • Publisher
      海文堂出版 (株)
    • Related Report
      2009 Final Research Report
  • [Book] ヒューマンインタフェースのための計測と制御2009

    • Author(s)
      山口昌樹, 編著(分担執筆)
    • Total Pages
      332
    • Publisher
      (株) シーエムシー出版
    • Related Report
      2009 Final Research Report
  • [Book] 心と体のセンシング2009

    • Author(s)
      ヒューマンサイエンスとセンシング調査研究委員会編, 山口昌樹(分担執筆)
    • Total Pages
      180
    • Publisher
      海文堂出版(株)
    • Related Report
      2009 Annual Research Report
  • [Book] ヒューマンインタフェースのための計測と制御23章児童生徒の心身ストレスの定量化と不登校対策2009

    • Author(s)
      山口昌樹編著(分担執筆)
    • Total Pages
      332
    • Publisher
      (株)シーエムシー出版
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ

    • URL

      http://www.mech.iwate-u.ac.jp/~yamaguchi/

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.mech.iwate-u.ac.jp/~yamaguchi/

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www3.u-toyama.ac.jp/yamag/index.htm

    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] 生体液分析器具, 及びかかる生体液分析器具を用いた生体液分析方法2009

    • Inventor(s)
      山口 昌樹
    • Industrial Property Rights Holder
      科学技術振興機構
    • Acquisition Date
      2009-05-01
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] アミラーゼ活性測定キット2009

    • Inventor(s)
      山口 昌樹, 吉田 博, 渥美 尚大, 木村 定明, 鈴木 一人, 東 朋幸, 松田 篤志, 草場 宣廷, 水野 康文, 橋本 政和
    • Industrial Property Rights Holder
      富山大学, ニプロ(株)
    • Acquisition Date
      2009-07-17
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] アミラーゼ活性測定キット2009

    • Inventor(s)
      山口昌樹, 吉田博, 渥美尚大, 木村定明, 鈴木一人, 東朋幸, 松田篤志, 草場宣廷, 水野康文, 橋本政和
    • Industrial Property Rights Holder
      富山大学,ニプロ(株)
    • Acquisition Date
      2009-07-17
    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] 新規コンジュゲートおよびそれを用いたコルチゾールの分析方法, キット, ならびにそのための装置2008

    • Inventor(s)
      山口 昌樹, 田原 祐助, 吉田 博
    • Industrial Property Rights Holder
      富山大学, ニプロ (株)
    • Patent Publication Number
      2009-210417
    • Filing Date
      2008-03-04
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 酵素センサおよびそれを用いた分析装置, 酵素センサの製造方法, ならびにアミラーゼ活性測定方法2008

    • Inventor(s)
      山口 昌樹, 小松 昭彦, 澤田 修一, 水野 康文, 鈴木 一人
    • Industrial Property Rights Holder
      ヤマハ (株), 富山大学
    • Acquisition Date
      2008-06-06
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] アミラーゼ活性測定用試薬, 測定方法, および装置2008

    • Inventor(s)
      山口 昌樹, 吉田 博, 草場 宜廷, 水野 康文
    • Industrial Property Rights Holder
      富山大学, ニプロ (株), ヤマハ発動機(株)
    • Acquisition Date
      2008-08-08
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 酵素センサおよびそれを用いた分析装置, 酵素センサの製造方法, ならびにアミラーゼ活性測定方法2008

    • Inventor(s)
      山口昌樹, 小松昭彦, 澤田修一, 水野康文, 鈴木一人
    • Industrial Property Rights Holder
      ヤマハ(株), 富山大学
    • Filing Date
      2008-06-06
    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] アミラーゼ活性測定用試薬, 測定方法, および装置2008

    • Inventor(s)
      山口昌樹, 吉田博, 草場宜廷, 水野康文
    • Industrial Property Rights Holder
      富山大学, ニプロ(株)
    • Filing Date
      2008-08-08
    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] 新規コンジュゲートおよびそれを用いたコルチゾールの分析方法, キット, ならびにそのための装置2008

    • Inventor(s)
      山口 昌樹, 田原 祐助, 吉田 博
    • Industrial Property Rights Holder
      富山大学ニプロ(株)
    • Industrial Property Number
      2008-053723
    • Filing Date
      2008-03-04
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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