New approach for detoxication of toxic substances in foods and the evaluation

• Project/Area Number: 19500675
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Eating habits, studies on eating habits
• Research Institution: National Institute of Health Sciences
• Principal Investigator: KONISHI Yoshiko National Institute of Health Sciences, 衛生微生物部, 部長 (10195761)
• Co-Investigator(Kenkyū-buntansha): 穐山 浩 国立医薬品食品衛生研究所, 代謝生化学部, 室長 (10260259)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: デトックス / 調理・加工 / 安全性 / カビ毒 / デオキシニバレノール / マウス / 吸収率 / ペクチンゲル / ペクチン / 減衰 / エッグボックス / ハーブ / ニバレノール

Research Abstract

Mycotoxin is natural contaminant and cause adverse effects on human and animal health. Since it is too hard for mycotoxin to reach zero tolerance, many approachs to reduce intake mycotoxin from final food products have been tried. However the developed tools until now has advantages and disadvantages. Therefore the development of new method for reduction of exposure to mycotoxins is needed on food processing.
In this study, we developed pectin gelation containing mycotoxin (DON) and examined suppressive effect on absorption of deoxynivalenol (DON). As pectin, low methoxy non-amidate (LMNA) pectin and amidate (LMA) pectin were used. The gelation using LMA pectin was effective for sealing DON into gel at 4℃ and 37℃. In vivo experiment revealed that the gelation suppressed the absorption of DON in gastro-intestine.
The LMA pectin would be valuable processing tool to reduce the exposure to mycotoxin.

Research Products

• [Journal Article] A survey of the occurrence of Fusarium Mycotoxins in Biscuits in Japan by using LC/MS. (2010)
  • Author(s): Tanaka, H., Sugita-Konishi, Y., Takino, M., Tanaka, T., Toriba, A., Hayakawa, K.
  • Journal Title: J. Health Science 56(2) Pages: 1-7
  • Peer Reviewed
• [Journal Article] A survey of the occurrence of Fusarium Mycotoxins in Biscuits in Japan by using LC/MS (2010)
  • Author(s): Tanaka, H., Sugita-Konishi, Y., Takino, M., Tanaka, T., Toriba, A., Hayakawa, K.
  • Journal Title: J.Health Science 56(2) Pages: 1-7
  • Peer Reviewed
• [Journal Article] Determination of nivalenol and deoxynivalenol by liquid chromatography/atmospheric pressure photoionization mass spectrometry. (2009)
  • Author(s): Tanaka H, Takino M, Sugita-Konishi Y, Tanaka T, Toriba A, Hayakawa K.
  • Journal Title: Rapid Commun Mass Spectrom 23(19) Pages: 3119-3124
  • Peer Reviewed
• [Journal Article] Determination of nivalenol deoxynivalenol by liquid chromatography/atmospheric pressure photoionization mass spectrometry (2009)
  • Author(s): Tanaka H, Takino M, Sugita-Konishi Y, Tanaka T, Toriba A, Hayakawa K.
  • Journal Title: Rapid Commun Mass Spectrom 23(19) Pages: 3119-3124
  • Peer Reviewed
• [Journal Article] Reducing rate A reduced rate of deoxynivalenol and nivalenol during bread production from wheat flour in Japan (2009)
  • Author(s): Sugiyama, K., Tanaka, H., Kamata, Y., Tanaka T., Sugita-Konishi, Y.
  • Journal Title: Mycotoxins 59 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Detection of deoxynivalenol contamination in wheat products in Thailand (2008)
  • Author(s): Poapolathep, A., Poapolathe p, S., Klangkaew, N., Sugita-Konishi, Y., Susumu Kumagao
  • Journal Title: J. Food Protection 71 Pages: 1931-1933
  • Peer Reviewed
• [Journal Article] Degradation of mycotoxins using microwave-induced argon plas ma at atmospheric pressure (2007)
  • Author(s): Park, B.J., Takatori, K.,. Sugita-Konishi, Y., Kim, I.K., Lee, M.H., Han, D.H., Chung, K.H., Hyun, S.O., Park, J.C.
  • Journal Title: Surface and Coating Technology 201 Pages: 5733-5737
  • Peer Reviewed
• [Journal Article] Specific detection of wheat residues in processed foods by polymerase chain reaction (2007)
  • Author(s): Yamakawa, H., Akiyama, H., Endo, Y., Miyatake, K, Sakata, K., Sakai, S., Toyoda, M., Urisu, A
  • Journal Title: Biosci, Biotechnol, and Biochem, 71 Pages: 2561-2564
  • NAID: 10027520225
  • Peer Reviewed
• [Presentation] Suppressive Effect of Pectin Gelation on Absorption of Deoxynivalenol in Mice. (2010)
  • Author(s): Sugita-Konishi, Y., Koyama, D., Kadota, T., Itoh, S., Sugiyama, K., Tamura, C., Nishijima, M. Kamata, Y.
  • Organizer: 49th Annual Meeting of Society of Toxicology
  • Place of Presentation: 米国、ソルトレイク
  • Year and Date: 2010-03-10
• [Presentation] Suppressive Effect of Pectin Gelation on Absorption of Deoxynivalenol in Mice (2010)
  • Author(s): Sugita-Konishi, Y., Koyama, D., Kadota, T., Itoh, S., Sugiyama, K., Tamura, C., Nishijima, M., Kamata, Y.
  • Organizer: 49th Annual Meeting of Society of Toxicology
  • Place of Presentation: 函館
  • Year and Date: 2010-03-10
• [Presentation] Suppressive Effect of Pectin Gelation on Absorption of Deoxynivalenol in Mice. (2009)
  • Author(s): Koyama, D., Arai, S., Kamata, Y., Nakajima, T., Sugita-Konishi, Y., Itoh, S.
  • Organizer: アジア養豚獣医学会(APVS2009)
  • Place of Presentation: 茨城県つくば市
  • Year and Date: 2009-10-27
• [Presentation] Study on the toxicokinetics of deoxynivalenol in swine (2009)
  • Author(s): Koyama, D., Arai, S., Kamata, Y., Nakajima, T., Sugita-Konishi, Y., Itoh, S.
  • Organizer: アジア養豚獣医学会(APVS2009)
  • Place of Presentation: 茨城県つくば市
  • Year and Date: 2009-10-27
• [Presentation] 低メトキシルペクチンのゲル化を利用したカビ毒の封入効果 (2009)
  • Author(s): 田村千佳子, 杉山圭一, 鎌田洋一, 小西良子, 中馬誠, 門田智之, 西島基弘
  • Organizer: 第98回日本食品衛生学会
  • Place of Presentation: 函館
  • Year and Date: 2009-10-08
• [Presentation] Sealing effects of pectin gelation on mycotoxin reduction in food (2009)
  • Author(s): Tamura, C., Nakamura, M., Kadota, T., Itoh, S., Kamata, Y., Sugiyama, K., Nishijima, N., Sugita-Konishi, Y
  • Organizer: ISM Conference 2009
  • Place of Presentation: ツーリン、オーストリア
  • Year and Date: 2009-09-08
• [Presentation] (2008)
  • Author(s): Sugita-Konishi, Y., Sugiyama, K., Saito S. Sato, T., Maragos, C., Kadota, T., Takezawa, Y., Kamata, Y., Tanaka, T., Sugita-Konishi, Y., Sugiyama, K., Saito S. Sato, T., Maragos, C., Kadota, T., Takezawa, Y., Kamata, Y., Tanaka, T.
  • Organizer: United-Japan Natural Resource Panel meeting
  • Place of Presentation: New orleans, USA
• [Presentation] Effect of deoxynivalenol on LPS signaling in macrophage (2007)
  • Author(s): Sugiyama, K., Muroi, M., Tanamoto', K., Nishijima, M., Sugita-Konishi'Y.
  • Organizer: Society of Toxicology
  • Place of Presentation: Siattle,USA
  • Year and Date: 2007-03-17
• [Book] Mycotoxins in Food, Feed and Bioweapons (2009)
  • Author(s): Yoshiko Sugiyta-Konishi (ed. Rai, Mahendra, Varma Ajit
  • Total Pages: 365
  • Publisher: Springer
• [Book] カビ対策ガイドブック (2007)
  • Author(s): 小西 良子
  • Total Pages: 158
  • Publisher: (社)日本食品衛生協会
• [Patent(Industrial Property Rights)] マイコトキシンの体内への吸収を抑制する効果のある食品または飼料用添加剤 (2009)
  • Inventor(s): 小西良子田村千佳子中馬誠
  • Industrial Property Rights Holder: 国立医薬品食品衛生研究所三栄源ffi(株)
  • Filing Date: 2009-09-08
  • Acquisition Date: 2009-09-08
• [Patent(Industrial Property Rights)] マイコトキシンの体内への吸収を抑制する効果のある食品または飼料用添加剤 (2009)
  • Inventor(s): 小西良子, 田村千佳子, 中馬誠
  • Industrial Property Rights Holder: 国立医薬品食品衛生研究所 三栄源FFI(株)
  • Industrial Property Number: 2009-207488
  • Filing Date: 2009-09-08