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Studies on the formation of basic ground of tideland ecosystem from the viewpoints of sediment protease activities.

Research Project

Project/Area Number 19510012
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental dynamic analysis
Research InstitutionPrefectural University of Hiroshima

Principal Investigator

NAKAMURA Kenichi  Prefectural University of Hiroshima, 生命環境学部, 教授 (50094694)

Co-Investigator(Kenkyū-buntansha) OKUNO Makoto  東京大, 大学院・総合文化研究科, 准教授 (40143325)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords河口干潟 / プロテアーゼ / 有機物質負荷 / 窒素代謝循環 / 干潟生態系 / 電気泳動法 / 有機物負荷 / 干潟 / 微生物 / 分泌酵素 / 植物プランクトン / 窒素循環 / 底質 / 生態系
Research Abstract

In tideland sediments, microorganisms secrete extracellular enzymes, which support waste purification and nutrient supplementation to plants and phytoplankton. Among the secreted enzymes, proteases are known to play essential roles in nitrogen cycling in coastal ecosystems. In this study, the protease activities of sediments were assayed at 22 sites in the Kamo River Estuary Tideland. The contour map of the activities showed spots of high activity located in two habitat areas for Uca lactea and Sargassum fulvellum. Moreover, statistical analysis indicated that the protease activity was correlated with the concentrations of organic carbon and nitrogen in the tideland sediments. Actually, loading of sediments with serum albumin as a protein substrate induced marked enhancement of protease activities without increasing of ATP biomass. Electrophoretic analysis clearly revealed the presence of protease bands induced by protein loading. These results suggest that protein loading stimulates the secretion of proteases by certain microorganisms.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (14 results)

All 2009 2008 2007

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (11 results)

  • [Journal Article] Regulation of sperm flagellar motility activation and chemotaxis caused by egg-derived substance(s) in sea cucumber sperm2009

    • Author(s)
      Morita, M., M. Kitamura, Nakajima, A. Susilo, E.S., Takemura, A., Okuno, M.
    • Journal Title

      Cell Motility and the Cytoskeleton Vol.68

      Pages: 2002-2015

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of sperm flagellar motility activation and chemotaxis caused by egg-derived substance(s)in sea cucumber sperm2009

    • Author(s)
      Morita, M.Okuno, M, et.al
    • Journal Title

      Cell Motility and the Cytoskeleton 66(4)

      Pages: 202-215

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 宇宙環境下における生殖・継世代研究の展開-IV宇宙利用シンポジウム報告書2008

    • Author(s)
      奥野誠, 中村健一
    • Journal Title

      日本学術会議

      Pages: 259-261

    • Related Report
      2009 Final Research Report
  • [Presentation] Detection and biochemical properties of proteolytic enzymes secreted by microalgae 9th International Phycological Congress2009

    • Author(s)
      Naito, C., Takase, M., Imai. Y., Nakamura, K.
    • Place of Presentation
      Tokyo
    • Year and Date
      2009-08-04
    • Related Report
      2009 Final Research Report
  • [Presentation] Detection and biochemical properties of proteolytic enzymes secreted by microalgae2009

    • Author(s)
      Naito, K., Nakamura, K., et.al
    • Organizer
      9th International Phycological Congress
    • Place of Presentation
      東京
    • Year and Date
      2009-08-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] 賀茂川河口干潟を対象とした底質プロテアーゼ分析とその生化学的特性2009

    • Author(s)
      中村健一, 中尾陽介, 内藤佳奈子
    • Organizer
      日本水環境学会
    • Place of Presentation
      山口大学
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] 藻類産生プロテアーゼの検出と生化学的特性2009

    • Author(s)
      内藤佳奈子, 高瀬真希, 今井一郎, 中村健一
    • Organizer
      日本藻類学会第33大会
    • Place of Presentation
      琉球大学
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] Iron utilizaion and biochemical properties of red tide-causing microalgae WFC2008 5th.2008

    • Author(s)
      Kanako Naito
    • Organizer
      The 5th World Fisheries Congress
    • Place of Presentation
      横浜市
    • Year and Date
      2008-10-21
    • Related Report
      2008 Annual Research Report
  • [Presentation] 広島県帝釈峡内神龍湖における微細藻類の増殖に対する鉄キレート物質の影響2008

    • Author(s)
      内藤, 村田, 中村, 山口, 今井
    • Organizer
      日本藻類学会第32回大会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2008-03-21
    • Related Report
      2007 Annual Research Report
  • [Presentation] Iron utilizaion and bio- chemical properties of red tide-causing microalgae WFC20082008

    • Author(s)
      Naito, K., Imai, I., Takase, M., Nakamura. K
    • Place of Presentation
      Yokohama
    • Year and Date
      2008-02-05
    • Related Report
      2009 Final Research Report
  • [Presentation] キウイフルーツに存在する高分子量プロテアーゼの電気泳動による解析2008

    • Author(s)
      増山悦子, 中村健一
    • Organizer
      第59会日本家政学会大会
    • Place of Presentation
      長良川国際会議
    • Related Report
      2009 Final Research Report
  • [Presentation] 広島県帝釈峡内神龍湖における微細藻類の増殖に対する鉄キレート物質の影響2008

    • Author(s)
      中村健一, 村田貴拓, 内藤千佳子
    • Organizer
      藻類学会第32回大会
    • Place of Presentation
      東京海洋大
    • Related Report
      2009 Final Research Report
  • [Presentation] 金属イオンによる種子発芽・成長への影響を観察できる教材開発2007

    • Author(s)
      和田 増山 中村
    • Organizer
      日本理科教育学会第57回大会
    • Place of Presentation
      愛知教育大学
    • Year and Date
      2007-08-05
    • Related Report
      2007 Annual Research Report
  • [Presentation] 種子発芽・発根に及ぼす銅イオンの作用を観察できる環境教育教材の開発2007

    • Author(s)
      和田 増山 中村
    • Organizer
      日本環境教育学会第18大会
    • Place of Presentation
      鳥取環境大学
    • Year and Date
      2007-05-25
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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