Complex of annexin II with cytoplasmic molecular chaperons is involved in novel DNA repair-regulating mechanisms

• Project/Area Number: 19510055
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Chiba University
• Principal Investigator: KITA Kazuko Chiba University, 大学院・医学研究院, 講師 (80302545)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Toshikazu 千葉大学, 大学院・医学研究院, 助教 (70270527) ; SUGAYA Shigeru 千葉大学, 大学院・医学研究院, 助教 (90334177)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: ヒト細胞 / 紫外線(UVC)抵抗性 / HSP27結合タンパク質 / annexin II / 損傷DNA修復 / シャペロン / 安定化 / 紫外線抵抗性 / annexinII

Research Abstract

短波長紫外線(UVC)ストレスに対応し、annexin II を実行因子、HSP27をシャペロンとするDNA損傷ストレス防御複合体が、UVC抵抗性に関わるとの仮説を立証すべく解析を行った。その結果、1) annexin II はHSP27 と複合体を形成すること、2) 両者の複合体は紫外線照射に応じて細胞質から核へ移行すること、3) annexin II がUVC致死抵抗性に関わることを明らかにした。さらに、4)SP27はシャペロンとしてannexin II の細胞内量の調節を介して紫外線致死抵抗化に関わる可能性を示唆した。

Research Products

• [Journal Article] Anti-proliferative and apoptosis-inducible activity of Sarcodonin G from Sarcodon scabrosus in HeLa cells (2008)
  • Author(s): Dong, M., Chen, S., Kita, K., Ichimura, Y., Guo, W., Lu, S., Sugaya, S., Hiwasa, T. Takiguchi, M., Mori, N., Kashima, A., Morimura, K., Hirota, M., Suzuki, N.
  • Journal Title: Int. J. Oncol. 34 Pages: 201-207
  • Peer Reviewed
• [Journal Article] Annexin II, a novel HSP27-interacted protein, is involved in resistance to UVC-induced cell death in human Ap^r-1 cells. (2008)
  • Author(s): Tong, X., Kita, K., Karata, K., Zhu, C. Sugaya, S., Ichimura, Y., Sato, M., Tomonaga, T., Nomura, F., Jin, Y., Suzuki, N.
  • Journal Title: Photochem. Photobiol. Pages: 1455-1461
  • Peer Reviewed
• [Journal Article] Down-regulation of molecular chaperone GRP78/BiP expression involved in enhancement of human RS cell mutability (2008)
  • Author(s): Hirano, J., Kita, K., Sugaya, S., Ichimura, Y., Yamamori, H., Nakajima, N., Suzuki, N.
  • Journal Title: Pancreas
  • Peer Reviewed
• [Journal Article] Down-regulation of molecular chaperone GRP78/Bip expression involved in enhancement of human RS cell mutability (2008)
  • Author(s): Hirano, J., Kita, K., Sugaya, S., Ichimura, Y., Yamamori, H., Nakajima, N., Suzuki, N.
  • Journal Title: Pancreas 36
  • Peer Reviewed
• [Journal Article] Annexin II, a novel HSP27-interacted protein, is involved in resistance to UVC-induced cell death in human AP^r-1 cells (2008)
  • Author(s): Tong, X., Kita, K., Karata, K., Zhu, C., Sugaya, S., Ichimura, Y., Sato, M., Tomonaga, T., Nomura, F., Jin, Y., Suzuki, N.
  • Journal Title: Photochem. Photobiol. 84 Pages: 1455-1461
  • Peer Reviewed
• [Journal Article] Anti-proliferative and apoptosis-inducible activity of Sarcodonin G from Sarcodon scabrosus in HeLa cells (2008)
  • Author(s): Dong, M., Chen, S., Kita, K., Ichimura, Y., Guo, W., Lu, S., Sugaya, S., Hiwasa, T., Takiguchi, M., Mori, N., Kashima, A., Morimura, K., Hirota, M., Suzuki, N.
  • Journal Title: Int. J. Oncol. 34 Pages: 201-207
  • Peer Reviewed
• [Journal Article] Down-regulation of molecular chaperone GRP78/BiP expression involved in enhancement of human RS cell mutability (2008)
  • Author(s): Hirano, J., Kita, K., Sugaya, S., Ichimura, Y, Yamamori, H., Nakajima, N., Suzuki, N.
  • Journal Title: Pancreas 36
  • Peer Reviewed
• [Journal Article] Reduction of GRP78 expression with siRNA activates unfolded protein response leading to apoptosis in HeLa cells (2007)
  • Author(s): Suzuki, T., Lu, J., Zahed, M., Kita, K., Suzuki, N.
  • Journal Title: Arch. Biochem. Biophys. 468 Pages: 1-14
  • Peer Reviewed
• [Journal Article] Modulation of mutability in UV-irradiated human RSb cells by serum obtained from parabolic flight volunteers (2007)
  • Author(s): Chi, X., Takahashi, S., Nomura, J., Sugaya, S., Ishimura, Y., Zhai,L., Tong, X., Kita, K., Suzuki, N.
  • Journal Title: J. Int. Soc. Life Info. Sci. 25 Pages: 11-19
  • NAID: 110006242228
  • Peer Reviewed
• [Journal Article] Moduation of mutability in UV-irradiated human RSB cells by serum obtained from parabolic flight voluteers (2007)
  • Author(s): Chi, X., Takahashi, S., Nomura, J. Sugaya, S., Ishimura, Y, Zhai, L., Ton, X., Kita, K., Suzuki, N.
  • Journal Title: J. Int. Soc. Life Info. Sci 25 Pages: 11-19
  • Peer Reviewed
• [Journal Article] Reduction of GRP78 expression with siRNA activates unfolded protein resonse leading to aotosis in HeLa cells (2007)
  • Author(s): Suzuki, T., Lu, J., Zahed, M., Kita K., Suzuki, N.
  • Journal Title: Arch. Biochem. Biophys 468 Pages: 1-14
  • Peer Reviewed
• [Journal Article] NM23-H1 is responsible for SUMO-2-involved DNA synthesis induction after X-ray irradiation in human cells
  • Author(s): Guo, W-Z., Sugaya, S., Satoh, M., Tomonaga, T., Nomura, F., Hiwasa, T., Takiguchi, M., Kita, K., Suzuki, N.
  • Journal Title: Arch. Biochem. Biophys. (in press)
  • Peer Reviewed
• [Journal Article] The roles of HSP27 and annexin II in resistance to UVC-induced cell death ; comparative studies between the human UVC-sensitive and -resistant cell lines, RSa and AP^r-1.
  • Author(s): Jin, Y-H., Kita, K., Sun, Z., Tong, X-B., Nie, H., Suzuki, N.
  • Journal Title: Biosci. Biotec. Biochem. (in press)
  • Peer Reviewed
• [Journal Article] Increase in the levels of chaperone proteins by exposure to β-estradiol, bisphenol A and 4-methoxyphenol in human cells transfected with estrogen receptor α cDNA
  • Author(s): Kita, K., Jin, Y-H., Sun, Z., Chen, S-P. Sumiya, Y., Hongo, T., Suzuki, N.
  • Journal Title: Toxicology in Vitro (in press)
  • Peer Reviewed
• [Journal Article] Increase in the levels of chaperone proteins by exposure to β-estradiol, bisphenol A and 4-methoxyphenol in human cells transfected with estrogen receptor αcDNA
  • Author(s): Kita, K., Jin, Y-H., Sun, Z., Chen, S-P., Sumiya, Y., Hongo, T., Suzuki, N.
  • Journal Title: Toxicology in Vitro (in press)
  • Peer Reviewed
• [Journal Article] The roles of HSP27 and annexin II in resistance to UVC-induced cell death:comparative studies between the human UVC-sensitive and -resistant cell lines, Rsa and AP^r-1
  • Author(s): Guo, W-Z., Sugaya, S., Satoh, M., Tomonaga, T., Nomura, F., Hisawa, T., Takiguchi, M., Kita, K., Suzuki, N.
  • Journal Title: Biosci. Biotec. Biochem., (in press)
  • Peer Reviewed
• [Journal Article] Nm23-H1 is responsible for SUMO-2-involved DNA synthesis induction after X-ray irradiation in human cells
  • Author(s): Guo, W-Z., Sugaya, S., Satoh, M., Tomonaga, T., Nomura, F., Hisawa, T., Takiguchi, M., Kita, K., Suzuki, N.
  • Journal Title: Arch. Biochem. Biophys., (in press)
  • Peer Reviewed
• [Journal Article] Annexin II, a novel HSP27-interacted protein, is involved in resistance to UVC-induced cell death in human AP^r-1 cells
  • Author(s): Tong, X., Kita. K., Karata, K., Zhu, C. Sugaya, S., Ichimura Y. Sato, M., Tomonaga, T., Nomura, F., Jin, Y., Suzuki, N.
  • Journal Title: Photochem. Photobiol (in press)
  • Peer Reviewed
• [Presentation] Annexin II, a novel HSP27-interacted protein, is involved in refractoriness of human cells to UVC lethality (2007)
  • Author(s): Tong, X., Kita, K., Karata, K., Zhu, C. Sugaya, S., Ichimura Y., Sato, M., Tomonaga, T., Nomura, F., Jin, Y., Suzuki, N.
  • Organizer: 第80回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
• [Presentation] ヒト細胞における放射線応答を制御するシャペロン複合体の探索-HSP27とannexin II複合体の発見 (2007)
  • Author(s): 金元虎、喜多和子、トウギョウハ、菅谷茂、佐藤守、朝長毅、野村文夫、鈴木信夫
  • Organizer: 第50回日本放射線影響学会
  • Place of Presentation: 幕張メッセ国際会議場
  • Year and Date: 2007-11-15
• [Presentation] HSP27 and its interacted protein, is involved in susceptibility to iradiation lethality in human cells (2007)
  • Author(s): Tong, X., Kita, K., Karata, K., Zhu, C. Sugaya, S., Ichimura Y.,Sato, M. Tomonaga, T., Nomura, F., Jin, Y., Suzuki, N.
  • Organizer: 2007 Keystone Symposia on Molecular and cellularBiology, Gastointestinal Cancer (T2)
  • Place of Presentation: Bejing, China
  • Year and Date: 2007-10-17
• [Presentation] HSP27 and its interacted protein,is involved in susceptibility to radiation lethality in human cells. (2007)
  • Author(s): Tong, X., Kita, K, Karata, K。 Zhu, C. Sugaya, S, Ichimura Y, Sato, M, Tomonaga, T, Nomura, F, Jin, Y, Suzuki, N.
  • Organizer: 2007 Keystone.Symposia on Molecular and cellular Biology, Gastointestinal Cancer(T2)
  • Place of Presentation: Bejing, China
  • Year and Date: 2007-10-17
• [Patent(Industrial Property Rights)] 紫外線防止剤 (2008)
  • Inventor(s): 鈴木信夫、戸村真澄、伊藤晴夫、菅谷茂、喜多和子、ロサ
  • Industrial Property Rights Holder: 千葉大学、株式会社I & C,伊藤晴夫
  • Industrial Property Number: 2008-327931
  • Filing Date: 2008-12-24
• [Patent(Industrial Property Rights)] 紫外線防止剤 (2008)
  • Inventor(s): 鈴木信夫, 戸村真澄, 伊藤晴夫, 菅谷茂, 喜多和子, 呂颯
  • Industrial Property Rights Holder: 千葉大学, 株式会社I&C, 伊藤晴夫
  • Industrial Property Number: 2008-327931
  • Filing Date: 2008-12-24