Analysis of function of PALB2, which binds to BRCA2(breast cancer susceptibility gene product 2), in homologous recombination

• Project/Area Number: 19510057
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Wakayama Medical University
• Principal Investigator: YAMAZOE Mitsuyoshi Wakayama Medical University, 医学部, 准教授 (00284745)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 相同DNA組換え / DNA修復 / 家族性乳癌 / BRCA2 / DT40細胞 / PALB2

Research Abstract

家族性乳癌原因因子BRCA2と、そのN末端領域に結合する因子PALB2の遺伝学的相互作用を、脊椎動物細胞で容易に遺伝子破壊を行うことができるニワトリBリンパ球DT40細胞を用いて解析した。PALB2の相同組換えにおける機能は、いずれもBRCA2の持つ機能の一部に含まれていた。このことからPALB2遺伝子はBRCA2遺伝子の下で、BRCA2と同じ相同組換え経路で働いていると結論した。

Research Products

• [Journal Article] ATP-induced shrinkage of DNA with MukB protein and the MukBEF complex of Escherichia coli (2008)
  • Author(s): Chen, N., Zinchenko, AA., Yoshikawa, Y., Araki, S., Adachi, S., Yamazoe, M., Hiraga, S. and Yoshikawa, K.
  • Journal Title: J Bacteriol 190 Pages: 3731-3737
  • Peer Reviewed
• [Journal Article] ATP-induced shrinkage of DNA with MukB protein and the MukBEF complex of Escherichia coli (2008)
  • Author(s): Chen N, Zinchenko AA, Yoshikawa Y, Araki S, Adachi S, Yamazoe M, Hiraga S, Yoshikawa K.
  • Journal Title: J Bacteriol. 190 Pages: 3731-3737
  • Peer Reviewed
• [Journal Article] Cells deficient in the FANC/BRCA pathway are hypersensitive to plasmalevel of formaldehyde (2007)
  • Author(s): Ridpath, JR., Nakamura, A., Tano, K.,Luke, AM., Sonoda, E., Arakawa, H., Buerstedde, JM., Gillespie, DA., Sale, JE., Yamazoe, M., Bishop, DK.,Takata, M., Takeda, S., Watanabe, M.,Swenberg, JA. and Nakamura, J
  • Journal Title: Cancer Res. 67 Pages: 11117-11122
  • Peer Reviewed
• [Journal Article] RAD51 up-regulation bypasses BRCA1 function and is a common feature of BRCA1-deficient breast tumors (2007)
  • Author(s): Martin, RW., Orelli, BJ., Yamazoe, M., Minn, AJ., Takeda, S. and Bishop, DK
  • Journal Title: Cancer Res. 67 Pages: 9658-9665
  • Peer Reviewed
• [Journal Article] RAD51 up-regulation bypasses BRCA1 function and is a common feature of BRCA1-deficient breast tumors. (2007)
  • Author(s): Martin RW, Orelli BJ, Yamazoe M, Minn AJ, Takeda S and Bishop DK
  • Journal Title: Cancer Res 67 Pages: 9658-9665
  • Peer Reviewed
• [Journal Article] Cells deficient in the FANC/BRCA pathway are hypersensitive to plasm a level of formaldehyde (2007)
  • Author(s): Ridpath JR, Nakamura A, Tano K, Luke AM, Sonoda E, Arakawa H, Buerstedde JM, Gillespie DA, Sale JE, Yamazoe M, Bishop DK, Takata M, Takeda S, Watanabe M, Swenberg JA and Nakamura J
  • Journal Title: Cancer Res 67 Pages: 11117-11122
  • Peer Reviewed