Detection of oxidative DNA damage induced by high LET radiation
| Project/Area Number |
19510059
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Tokai University |
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Principal Investigator |
ITO Atsushi Tokai University, 工学部, 教授 (80193473)
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| Co-Investigator(Kenkyū-buntansha) |
村山 千恵子 東海大学, 医学部, 講師 (50307295)
平山 亮一 独立行政法人放射線医学総合研究研究員所, 重粒子医科学センター, 研究員 (90435701)
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| Co-Investigator(Renkei-kenkyūsha) |
MURAYAMA Chieko 東海大学, 医学部, 講師 (50307295)
HIRAYAMA Ryoichi 独立行政法人放射線医学総合研究所, 重粒子医科学センター, 研究員 (90435701)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2007: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 高LET放射線 / 重イオンビーム / 間接作用 / ラジカル / DNA酸化損傷 / 酸素効果 / トラック構造 / 8-OHdG / 酸化損傷 / 8-oxoG / 蛍光抗体法 |
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| Research Abstract |
Production of 8-hydroxy-2'-deoxyguanosine (8-OHdG), an OH radical induced base damage, was examined upon irradiation with carbon, neon, silicon and iron ions over an LET range from 20 to 440keV/μm under oxic and hypoxic conditions. 8-OHdG was detected by HPLC and an electro-chemical detector after extraction of DNA from irradiated cells. The yield of 8-OHdG was decreased with increasing LET under oxic condition, and strongly depended on ion species. Under hypoxic condition the yield appeared not to decrease monotonically but to approach constant level or rather to increase in the high LET region. This observation is consistent with the oxygen-in-the-track model where oxygen molecules are supposed to be produced along ion tracks. To visualize the distribution of 8-OHdG along ion tracks we imaged 8-OHdG distribution using FITC-labeled 8-OHdG antibody. At a dose as small as 5Gy, 8-OHdG was detected for every ion.
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Report
(4 results)
Research Products
(44 results)
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[Journal Article] Contributions of direct and indirect actions in cell killing by high-LET radiations2009
Author(s)
R. Hirayama, A. Ito, M. Tomita, T. Tsukada, F. Yatagai, M. Noguchi, Y. Matsumoto, Y. Kase, K., o, R. Okayasu, Y. Furusawa
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Journal Title
Radiat. Res. 171
Pages: 212-218
Related Report
Peer Reviewed
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[Presentation] Induction of DNA dsb and its rejoining in the clamped and non-clamped tumors after carbon ion beams compared with X-rays2009
Author(s)
R. Hirayama, A. Uzawa, Y. Matsumoto, M. Noguchi, Y. Kase, A. Ito, R. Okayasu, S. Koike, K. Ando, Y. Furusawa
Organizer
MICROS 2009 15th International Symposium on Microdosimetry
Place of Presentation
Verona, Italy
Year and Date
2009-10-29
Related Report
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