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Studies of the utilization of the sludge dredged from brackish-water region by halo-alkaliphilic bacteria

Research Project

Project/Area Number 19510087
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental technology/Environmental materials
Research InstitutionShimane University

Principal Investigator

OSHIMA Akinobu  Shimane University, 生物資源科学部, 准教授 (70152113)

Co-Investigator(Kenkyū-buntansha) ISHIDA Akio  熊本大学, 自然科学研究科, 教授 (40040117)
NAGATA Shinichi  神戸大学, 内海域環境教育研究センター, 教授 (10108847)
SASAKI Hideaki  いわき明星大学, 科学技術学部, 助教 (30405998)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords好アルカリ性細菌 / 腐泥 / 有機物分解 / 環境修復
Research Abstract

About 7,200 colonies of halo-alkaliphilic bacteria were isolated from the samples collected from 4 sources including Lake Nakaumi in Shimane Prefecture. 127 colonies with high decomposition ability of organic compounds were selected by second screening. All bacteria isolated belong to the genus Bacillus. After alkalinized the sludge by the addition of CaO, selected strains were added to the sludge and decomposition of organic compound were examined. As a result of the incubation, we found that elution of phosphate was decreased sharply and about 20% of organic carbon was decreased.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (43 results)

All 2010 2009 2008 2007

All Journal Article (25 results) (of which Peer Reviewed: 20 results) Presentation (16 results) Book (2 results)

  • [Journal Article] Degradation of organic compounds in sludge dredged from brackish-water region by halo-alkaliphilic bacteria II2010

    • Author(s)
      Fukuda N, Ishida A, Nagata S, Sasaki H, Oshima A
    • Journal Title

      Proceedings of the 46th Conference on the Biology of Halophilic Microorganisms, Japan (in press)

    • Related Report
      2009 Final Research Report
  • [Journal Article] Ectoine Synthesis and Proline Uptake are Involved in Osmoadaptation of an Alkaliphilic Bacillus sp.2010

    • Author(s)
      Oshima A, Munetoh M, Ishida A, Sasaki H, Nagata, S
    • Journal Title

      U-21 Advances in Medicine and Biology. 4(in press)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Degradation of organic compounds in sludge dredged from brackish-water region by halo-alkaliphilic bacteria II2010

    • Author(s)
      Fukuda N,
    • Journal Title

      Proceedings of the 46^<th> Conference on the Biology of Halophilic Microorganisms, Japan (印刷中)

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Ectoine Synthesis and Proline Uptake are Involved in Osmoadaptation of an Alkaliphilic Bacillus sp. U-212010

    • Author(s)
      Oshima A,
    • Journal Title

      Advances in Medicine and Biology 4(印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of alginate-degrading bacteria for disposal of seaweed wastes2009

    • Author(s)
      Tang J C, Taniguchi H, Chu H, Zhou Q, Nagata S
    • Journal Title

      Lett. Appl. Microbiol. 48

      Pages: 38-43

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Degradation of organic compounds in sludge dredged from brackish-water region by halo-alkaliphilic bacteria2009

    • Author(s)
      Fukuda N, Ishida A, Nagata S, Sasaki H, Oshima A
    • Journal Title

      Proceedings of the 45th Conference on the Biology of Halophilic Microorganisms, Japan

      Pages: 21-23

    • Related Report
      2009 Final Research Report
  • [Journal Article] Importance of the transcription of Proline transporter ProP gene in quick adaptation of Escherichia coli cells under high salinity2009

    • Author(s)
      Sasaki H,
    • Journal Title

      Bulletin of the Society of Sea Water Science, Japan 63

      Pages: 338-342

    • NAID

      10025408280

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of extreme halotolerant bacteria from Asian desert dust ; molecular phylogeny and growth properties of their cells2009

    • Author(s)
      Sasaki H,
    • Journal Title

      Research Journal of Microbiology 4

      Pages: 260-268

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of alginate-degrading bacteria for disposal of seaweed wastes2009

    • Author(s)
      Tang J C,
    • Journal Title

      Lett.Appl.Microbiol. 48

      Pages: 38-43

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Degradation of organic compounds in sludge dredged from brackish-water region by halo-alkaliphilic bacteria2009

    • Author(s)
      Fukuda N,
    • Journal Title

      Proceedings of the 45^<th> Conference on the Biology of Halophilic Microorganisms, Japan

      Pages: 21-23

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Disposal of seaweed wakame (Undaria pinnatifida) in composting process by marine bacterium Halomonas sp. AW42008

    • Author(s)
      Tang J.C., Xiao Y., Oshima A., Kawai H., Nagata S
    • Journal Title

      International Journal of Biotechnology 72

      Pages: 73-85

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Efficient cyclic system to yield ectoine using Brevibacterium sp. JCM 6894 subjected to osmotic downshock.2008

    • Author(s)
      Nagata S, Wang Y Q, Oshima A, Zhang L, Miyake H, Sasaki H, Ishida A
    • Journal Title

      Biotechnology and Bioengineering 99

      Pages: 941-948

    • NAID

      120000942853

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of alkaliphilic bacteria from seashore and brackish water region2008

    • Author(s)
      Oshima A, Ishida A, Nagata S, Sasaki H
    • Journal Title

      Proceedings of the 44th Conference on the Biology of Halophilic Microorganisms, Japan

      Pages: 21-23

    • Related Report
      2009 Final Research Report
  • [Journal Article] Disposal of seaweed wakame (Undaria pinnatifida)in composting process by marine bacterium Halomonas sp. AW4.2008

    • Author(s)
      Tang J C
    • Journal Title

      International Journal of Biotechnology 10

      Pages: 73-85

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of salt-sensitive mutants of the moderately halophilic bacterium Salinivibrio costicola subsp. yaniae2008

    • Author(s)
      Zhu D
    • Journal Title

      Bioscience, Biotechnology and Biochemistry 72

      Pages: 1977-1982

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient cyclic system to yield ectoine using Brevibacteriumsp. JCM 6894 subjected to osmotic downshock.2008

    • Author(s)
      Nagata s
    • Journal Title

      Biotechnology and Bioengineering 99

      Pages: 941-948

    • NAID

      120000942853

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phylogenetic identification of epibiotic bacteria possessing antimicrobial activities isolated from red algal species of Japan.2008

    • Author(s)
      Kanagasabhapathy M
    • Journal Title

      World Journal of Microbiology & Biotechnology 24

      Pages: 2315-2321

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient cyclic system to yield ectoine using Brevibacterium sp. JCM 6894 subjected to osmotic downshock.2008

    • Author(s)
      Nagata S,
    • Journal Title

      Biotechnology and Bioengineering 99

      Pages: 941-948

    • NAID

      120000942853

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient cyclic system to yield ectoine using Brevibacterium sp. JCM 6894 subjected to osmotic downshock2008

    • Author(s)
      Nagata S,
    • Journal Title

      Biotechnology and Bioengineering 99

      Pages: 941-948

    • NAID

      120000942853

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of the function of L-and D-proline as compatible solute in Escherichia coli K-12 under high osmolarity.2007

    • Author(s)
      Sasaki H, Takagi A, Oshima A, Ishida A, Nagata S
    • Journal Title

      Annals of Microbiology 57

      Pages: 265-268

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Degradation of seaweed Wakame (Undaraia pinnatifida) by composting process with inoculation of Bacillus sp. HR6.2007

    • Author(s)
      Tang, J. C, Wei J H, Maeda K, Kawai H, Zhou Q, Hosoi-Tanabe S, Nagata S.
    • Journal Title

      Biocontrol Science 12

      Pages: 47-54

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of moderately halophilic bacterium Halomonas ventosae DL7 synthesizing ectoine as compatible solute2007

    • Author(s)
      Zhu D, Niu L, Wang C X, Nagata S
    • Journal Title

      Annales of Microbiology 57

      Pages: 401-406

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of the function of L-and D-proline as compatible solute in Escherichia coli K-12 under high osmolarity.2007

    • Author(s)
      Sasaki H,
    • Journal Title

      Annals of Microbiology 57

      Pages: 265-268

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Degradation of seaweed Wakame(Undaria pinnatifida)by composting process with inoculation of Bacillus sp. HR62007

    • Author(s)
      Tang J C,
    • Journal Title

      Biocontrol Science 12

      Pages: 47-54

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of moderately halophilic bacterium Halomonas ventosae DL7 synthesizing ectoine as compatible solute2007

    • Author(s)
      Zhu D,
    • Journal Title

      Annales of Microbiology 57

      Pages: 401-406

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 好塩好アルカリ性細菌を用いた汽水域生腐泥中の有機物分解についてII2009

    • Author(s)
      福田直登
    • Organizer
      好塩微生物研究会第45回大会
    • Place of Presentation
      帝塚山大学
    • Year and Date
      2009-12-13
    • Related Report
      2009 Final Research Report
  • [Presentation] 好塩好アルカリ性細菌を用いた汽水域生腐泥中の有機物分解についてII2009

    • Author(s)
      福田直登
    • Organizer
      好塩微生物研究会 第45回大会
    • Place of Presentation
      帝塚山大学
    • Year and Date
      2009-12-13
    • Related Report
      2009 Annual Research Report
  • [Presentation] 好塩好アルカリ性細菌を用いた汽水域生腐泥の処理について2009

    • Author(s)
      福田直登
    • Organizer
      日本植物学会第73回大会
    • Place of Presentation
      山形大学
    • Year and Date
      2009-09-19
    • Related Report
      2009 Final Research Report
  • [Presentation] 好塩好アルカリ性細菌を用いた汽水域生腐泥の処理について2009

    • Author(s)
      福田直登
    • Organizer
      日本植物学会 第73回大会
    • Place of Presentation
      山形大学
    • Year and Date
      2009-09-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] 好塩好アルカリ性細菌を用いた汽水域生腐泥中の有機物分解について2008

    • Author(s)
      福田直登
    • Organizer
      好塩微生物研究会第45回大会
    • Place of Presentation
      帝塚山大学
    • Year and Date
      2008-12-13
    • Related Report
      2009 Final Research Report
  • [Presentation] 好塩好アルカリ性細菌を用いた汽水域生腐泥中の有機物分解について2008

    • Author(s)
      福田直登
    • Organizer
      第45回好塩微生物研究会
    • Place of Presentation
      帝塚山大学
    • Year and Date
      2008-12-13
    • Related Report
      2008 Annual Research Report
  • [Presentation] 好アルカリ性細菌を用いた汽水域生腐泥の利用について2008

    • Author(s)
      福田直登
    • Organizer
      極限環境微生物学会
    • Place of Presentation
      立教大学
    • Year and Date
      2008-11-04
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] 海浜及び汽水域から分離した好アルカリ性細菌を用いた汽水域生腐泥の利活用について2008

    • Author(s)
      福田直登
    • Organizer
      日本植物学会第72回大会
    • Place of Presentation
      高知大学
    • Year and Date
      2008-09-26
    • Related Report
      2009 Final Research Report
  • [Presentation] 海浜及び汽水域から分離した好アルカリ性細菌を用いた汽水域生腐泥の利活用について2008

    • Author(s)
      福田直登
    • Organizer
      日本植物学会 第72回大会
    • Place of Presentation
      高知大学
    • Year and Date
      2008-09-26
    • Related Report
      2008 Annual Research Report
  • [Presentation] 海浜及び汽水域からの高い有機物分解活性を示す好アルカリ性細菌の分離について2008

    • Author(s)
      大島朗伸
    • Organizer
      日本植物学会中国四国支部大会
    • Place of Presentation
      広島大学
    • Year and Date
      2008-05-17
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] Isolation and utilization of extremophiles form the sediment of brackish-water regions2008

    • Author(s)
      Oshima A
    • Organizer
      International Symposium Restoration and Management of Wetlands
    • Place of Presentation
      島根大学
    • Year and Date
      2008-03-02
    • Related Report
      2009 Final Research Report
  • [Presentation] Isolation and utilization of extremophiles form the sediment of brackish-water regions2008

    • Author(s)
      Oshima A.
    • Organizer
      International Symposium Restoration and Management of Wetlands
    • Place of Presentation
      Shimane University
    • Year and Date
      2008-03-02
    • Related Report
      2007 Annual Research Report
  • [Presentation] 海浜及び汽水域からの好アルカリ性細菌の分離について2007

    • Author(s)
      大島朗伸
    • Organizer
      好塩微生物研究会第44回大会
    • Place of Presentation
      熊本大学
    • Year and Date
      2007-12-15
    • Related Report
      2009 Final Research Report
  • [Presentation] 海浜及び汽水域からの好アルカリ性細菌の分離について2007

    • Author(s)
      大島朗伸
    • Organizer
      好塩微生物研究会 第44回大会
    • Place of Presentation
      熊本大学
    • Year and Date
      2007-12-15
    • Related Report
      2007 Annual Research Report
  • [Presentation] 中海底質より分離した好アルカリ性細菌No. 4545株の菌体外プロテアーゼについて2007

    • Author(s)
      大島朗伸
    • Organizer
      日本植物学会中国四国支部大会
    • Place of Presentation
      鳥取大学
    • Year and Date
      2007-05-15
    • Related Report
      2009 Final Research Report
  • [Presentation] 中海底質より分離した好アルカリ性細菌No.4545株の菌体外プロテアーゼについて2007

    • Author(s)
      大島朗伸
    • Organizer
      日本植物学会中国四国支部大会
    • Place of Presentation
      鳥取大学
    • Year and Date
      2007-05-15
    • Related Report
      2007 Annual Research Report
  • [Book] Diamino Amino Acid2008

    • Author(s)
      Nagata S.
    • Publisher
      Nova Science Publishers
    • Related Report
      2009 Final Research Report
  • [Book] Diamino Amino Acid2008

    • Author(s)
      Nagata s.
    • Publisher
      Nova Science Publishers
    • Related Report
      2008 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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