Selective targeting of novel multifunctionalized bionanosilioa particles to cell

• Project/Area Number: 19510117
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: The University of Tokushima
• Principal Investigator: NAKAMURA Michihiro The University of Tokushima, 大学院・ヘルスバイオサイエンス研究部, 准教授 (10314858)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ナノ多機能材料 / ナノバイオ / ナノ材料 / ナノ粒子 / 有機シリカ / イメージング / 抗体 / ドラッグデリバリー / シリカ / 蛋白質

Research Abstract

新しい多機能バイオナノシリカ粒子を作製し、標的とする細胞ヘターゲッティングを検討した。粒子作製においてサイズ分布が均一なナノ粒子、様々な蛍光シグナルや表面電荷を持つ粒子、抗体を表面に結合した粒子の作製が可能となった。多機能バイオナノシリカ粒子は粒子サイズや粒子表面特性の違いにより様々な様式で細胞ヘターゲットすることが電子顕微鏡などの形態学的評価で確認できた。

Research Products

• [Journal Article] Size-controlled, One-pot Synthesis, Characterization, and Biological Applications of Epoxy-Organosilica Particles Possessing Positive Zeta Potential as Prepared (2008)
  • Author(s): Nakamura, M., Ishimura, K
  • Journal Title: Langmuir 24 Pages: 12228-12234
  • Peer Reviewed
• [Journal Article] One-pot synthesis and characterization of three kinds of thiol-organosilica nanoparticles (2008)
  • Author(s): Nakamura, M., Ishimura, K
  • Journal Title: Langmuir 24 Pages: 5099-5108
  • Peer Reviewed
• [Journal Article] One-pot synthesis and characterization of three kinds of thiol-organosilica nanoparticles. (2008)
  • Author(s): Nakamura, M., et al.
  • Journal Title: Langmuir 24 Pages: 5099-5108
  • Peer Reviewed
• [Journal Article] Size-controlled, one-pot synthesis, charaoterization, and biological applications of epoxy-organosilica particles possessing positive zeta potential as prepared. (2008)
  • Author(s): Nakamura, M., et al.
  • Journal Title: Langmuir 24 Pages: 12228-12234
  • Peer Reviewed
• [Journal Article] One-pot synthesis and characterization of three kinds of thiol-organosilica nanoparticles. (2008)
  • Author(s): Michihiro Nakamura
  • Journal Title: Langmuir (in press)
  • Peer Reviewed
• [Journal Article] ovel drug delivery system of hollow mesoporous silica nanocapsules with thin shells : Preparation and fluorescein isothiocyanate(FITC)release kinetics (2007)
  • Author(s): Liu Y, Miyoshi El, Nakamura M
  • Journal Title: Colloids and Surfaces B : Biointerfaces 58 Pages: 180-187
  • Peer Reviewed
• [Journal Article] Synthesis and characterization of organosilica nanoparticles prepared from 3-mercaptopropyltrimethoxysilane as the single silica source (2007)
  • Author(s): Nakamura, M., Ishimura, K
  • Journal Title: Journal of Physical Chemistry C 111 Pages: 18892-18898
  • Peer Reviewed
• [Journal Article] ynthesis, characterization, and biological applications of multifluorescent silica nanoparticles (2007)
  • Author(s): Nakamura, M., Shone, M., Ishimura, K
  • Journal Title: Analytical Chemistry 79 Pages: 6507-6514
  • Peer Reviewed
• [Journal Article] Nanomedicine for drug delivery and imaging : A promising avenue for cancer therapy and diagnosis using targeted functional nanoparticles (2007)
  • Author(s): Liu Y, Miyoshi H, Nakamura M
  • Journal Title: nternational Journal of Cancer 120 Pages: 2527-2537
  • Peer Reviewed
• [Journal Article] Nanomedicine for drug delivery and imaging:A promising avenue for cancer therapy and diagnosis using targeted functional nanoparticles. (2007)
  • Author(s): Yiyao Liu
  • Journal Title: International Journal of Cancer 120 Pages: 2527-2537
  • Peer Reviewed
• [Journal Article] Novel drug delivery system of hollow mesoporous silica nanocapsules with thin shells: Preparation and fluorescein isothiocyanate(FITC)xelease kinetics. (2007)
  • Author(s): Yiyao Liu
  • Journal Title: Colloids and Surfaces B:Biointerfaces 58 Pages: 180-187
  • Peer Reviewed
• [Journal Article] Synthesis,characterization, and biological applications of multifluorescent silica nanoparticles (2007)
  • Author(s): Michihiro Nakamura
  • Journal Title: Analytical Chemistry 79 Pages: 6507-6514
  • Peer Reviewed
• [Journal Article] Synthesis and characterization of organosilica nanoparticles prepared from 3-mercaptopropyltrimethoxysilane as the single silica source. (2007)
  • Author(s): Michihiro Nakamura
  • Journal Title: Journal of Physical Chemistry C 111 Pages: 18892-18898
  • Peer Reviewed
• [Presentation] 有機シリカ粒子と有機シリカ粒子技術 (2009)
  • Author(s): 中村教泰
  • Organizer: nano tech 2009国際ナノテクノロジー総合展・技術会議
  • Place of Presentation: 東京ビッグサイト(東京都江東区有明)
• [Presentation] 有機シリカ粒子と有機シリカ粒子技術 (2009)
  • Author(s): 中村教泰
  • Organizer: nano tech 2009 国際ナノテクノロジー総合展・技術会議
  • Place of Presentation: 東京ビッグサイト
• [Presentation] 有機シリカ粒子技術による新規なイメージングプローブの開発 (2008)
  • Author(s): 中村教泰、石村和敬
  • Organizer: 第17回日本バイオイメージング学会
  • Place of Presentation: 千葉大学けやき会館(西千葉地区)
  • Year and Date: 2008-11-11
• [Presentation] 有機シリカ粒子技術による新規なイメージングプローブの開発 (2008)
  • Author(s): 中村教泰, et al.
  • Organizer: 第17回日本バイオイメージング学会
  • Place of Presentation: 千葉大学けやき会館
  • Year and Date: 2008-11-01
• [Presentation] 新規な有機ナノシリカ粒子の作製と光線力学的治療への応用 (2008)
  • Author(s): 中村教泰、石村和敬
  • Organizer: 第18回日本光線力学学会
  • Place of Presentation: テレピアホール(愛知県名古屋市東区東桜)
  • Year and Date: 2008-06-14
• [Presentation] 新規な有機ナノシリカ粒子の作製と光線力学的治療への応用 (2008)
  • Author(s): 中村教泰, et al.
  • Organizer: 第18回日本光線力学学会
  • Place of Presentation: テレピアホール
  • Year and Date: 2008-06-14
• [Presentation] 新規な有機ナノシリカ粒子の作製と多機能化 (2008)
  • Author(s): 中村教泰、石村和敬
  • Organizer: ナノ学会第6回大会
  • Place of Presentation: 九州大学医学部百周年記念講堂
  • Year and Date: 2008-05-07
• [Presentation] 新規な有機シリカナノ粒子の作製とDDSへの応用 (2008)
  • Author(s): 中村教泰、石村和敬
  • Organizer: 第24回日本DD5学会
  • Place of Presentation: 六本木アカデミーヒルズ(東京都港区六本木)
• [Presentation] 新規な有機シリカナノ粒子の作製とDDSへの応用 (2008)
  • Author(s): 中村教泰, et al.
  • Organizer: 第24回日本DDS学会
  • Place of Presentation: 六本木アカデミーヒルズ
• [Presentation] Preparation of novel organosilica nanoparticles with fluorescent andtheir functionalization with biomolecules. (2007)
  • Author(s): Michihiro Nakamura
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
• [Presentation] Preparation and characterization of novel biofunctionalized fluorescentsilica nanoparticles and their possibility for photodynamic therapy. (2007)
  • Author(s): Michihiro Nakamura
  • Organizer: 12th Congress of the European Society for Photobiology
  • Place of Presentation: University of Bath, U.K.
  • Year and Date: 2007-09-05
• [Presentation] Preparation and characterization of novel biofunctionalized fluorescent silica nanoparticles and their possibility for photodynamic therapy (2007)
  • Author(s): ichihiro Nakamura, Masayuki Shono, Kazunori Ishimura
  • Organizer: 12th Congress of the European Society for Photobiology
  • Place of Presentation: University of Bath, U.K.
• [Presentation] 蛍光色素含有ナノシリカ粒子の作製と評価 (2007)
  • Author(s): 中村教泰、石村和敬
  • Organizer: BMB2007(第30回日本分子生物学会年会・第80回日本生化学会大会合同大会)
  • Place of Presentation: パシフィコ横浜
• [Book] Approaches to Biofunctionalization of Spherical Silica Nanomaterials. In : Kumar C.S.S.R., editior. Nanomaterial for Life Sciences Volume 2 : Nanostructured Oxides. Wiley-VCH Verlag GmbH & Co (2009)
  • Author(s): Nakamura, M
  • Publisher: KGaA, Weinheim, Germany
• [Book] Approaches to Biofunctionalization of Spherical Silica Nanomaterials. In : Kumar C. S. S. R., editior. Nanomaterial for Life Sciences Volume 2 : Nanostructured Oxides. (2009)
  • Author(s): Nakamura, M.
  • Publisher: Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany
• [Patent(Industrial Property Rights)] ナノ機能性シリカ粒子およびその製造方法 (2008)
  • Inventor(s): 中村教泰
  • Industrial Property Rights Holder: 徳島大学
  • Filing Date: 2008-12-08
  • Overseas
• [Patent(Industrial Property Rights)] フローサイトメトリーによる生体分子の定量又は細胞の検出・分取に用いる蛍光シリカ粒子、及び複数個の蛍光シリカ粒子を組み合わせたキット (2008)
  • Inventor(s): 會澤英樹、大久保典雄、中村教泰、三好弘一
  • Industrial Property Rights Holder: 徳島大学、古河電気工業株式会社
  • Industrial Property Number: 2008-177413
  • Filing Date: 2008-07-07
• [Patent(Industrial Property Rights)] フローサイトメトリーによる細胞の検出・分取システム、及び検出上分取方法 (2008)
  • Inventor(s): 會澤英樹、大久保典雄、中村教泰、三好弘一
  • Industrial Property Rights Holder: 徳島大学、古河電気工業株式会社
  • Industrial Property Number: 2008-177409
  • Filing Date: 2008-07-07
• [Patent(Industrial Property Rights)] フローサイトメトリーによる生体分子の定量又は細胞の検出・分取に用いる蛍光シリカ粒子、及び複数個の蛍光シリカ粒子を組み合わせたキット (2008)
  • Inventor(s): 會澤英樹, 大久保典雄, 中村教泰, 三好弘
  • Industrial Property Rights Holder: 徳島大学古河電気工業株式会社
  • Industrial Property Number: 2008-177413
  • Filing Date: 2008-07-07
• [Patent(Industrial Property Rights)] フローサイトメトリーによる細胞の検出・分取システム、及び検出・分取方法 (2008)
  • Inventor(s): 會澤英樹, 大久保典雄, 中村教泰, 三好弘一
  • Industrial Property Rights Holder: 徳島大学古河電気工業株式会社
  • Industrial Property Number: 2008-177409
  • Filing Date: 2008-07-07
• [Patent(Industrial Property Rights)] ナノ機能性シリカ粒子およびその製造方法 (2008)
  • Inventor(s): 中村教泰
  • Industrial Property Rights Holder: 徳島大学
  • Filing Date: 2008-12-08
  • Overseas
• [Patent(Industrial Property Rights)] 多機能性を有する新規なナノシリカ粒子の製造方法と用途 (2007)
  • Inventor(s): 中村教泰
  • Industrial Property Rights Holder: 徳島大学
  • Industrial Property Number: 2007-316466
  • Filing Date: 2007-12-06
• [Patent(Industrial Property Rights)] 新規なナノシリカ粒子の製造方法と用途 (2007)
  • Inventor(s): 中村教泰
  • Industrial Property Rights Holder: 徳島大学
  • Filing Date: 2007-06-07
  • Overseas
• [Patent(Industrial Property Rights)] 新規なナノシリカ粒子の製造方法と用途 (2007)
  • Inventor(s): 中村 教泰
  • Industrial Property Rights Holder: 徳島大学
  • Filing Date: 2007-06-07
  • Overseas
• [Patent(Industrial Property Rights)] 多機能性を有する新規なナノシリカ粒子の製造方法と用途 (2007)
  • Inventor(s): 中村 教泰
  • Industrial Property Rights Holder: 徳島大学
  • Industrial Property Number: 2007-316466
  • Filing Date: 2007-12-06