Development of reversible manipulation methods for single biomolecules by using scanning probe microscopy

• Project/Area Number: 19510127
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Tokyo University of Science (2008); Musashi Institute of Technology (2007)
• Principal Investigator: UMEMURA Kazuo Tokyo University of Science, 理学部第二部・物理学科, 准教授 (60281664)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 原子間力顕微鏡 / 環境応答性 / ナノデバイス / 表面修飾 / 原子間力顕微 / 分子操作 / 環境応答 / カンチレバー / フォースカーブ / 温度応答 / タンパク質 / 熱ドリフ / ポリマー

Research Abstract

本研究では、温度応答性ポリマー等の環境応答性分子を用いて、原子間力顕微鏡(AFM)等による単一分子操作等技術に役立つ知見、特にAFM探針の先端での「可逆」な応答が可能な実験系の構築を行った。その結果、AFM探針にタンパク質分子を吸着させた状態で、温度を変化させながらポリマー表面への吸着力を制御することに成功した。最近開発されたプラトー探針を用いると極めて安定した測定が可能となり、10回温度変化を繰り返しても可逆な制御が可能だった。

Research Products

• [Journal Article] Nanoscopic deformation of a nafion film surface caused by annealing studied using atomic force microscopy and a patterned substrate (2008)
  • Author(s): Y Maeda, Y Gao, M Nagai, Y Nakayama, T Ichinose, R Kuroda, K Umemura
  • Journal Title: Ultramicroscopy 108 Pages: 529-535
  • Peer Reviewed
• [Journal Article] Direct observation of deformation of nafion surfaces induced by methanol treatment by using atomic force microscopy (2008)
  • Author(s): Kazuo Umemura, Reiko Kuroda, Yanfeng Gao, Masayuki Nagai, Yuta Maeda
  • Journal Title: Appl. Surf. Sci. 254, 23 Pages: 7980-7984
• [Journal Article] Nanoscopic deformation of a nafion film surface caused by annealing studied using atomic force microscopy and a patterned substrate. (2008)
  • Author(s): Y Maeda, Y Gao, M Nagai, Y Nakayama, T Ichinose, R Kuroda, K Umemura
  • Journal Title: Ultramicroscopy 108 Pages: 529-535
  • Peer Reviewed
• [Presentation] In situ imaging and force measurements of an poly(N isopropylacrylamide) surface by atomic force microscopy (2009)
  • Author(s): Kazuo Umemura, Reiko Kuroda, Akihiko Kikuchi
  • Organizer: Asia Biophysics Symposium
  • Place of Presentation: Hongkong Science and Technology University, Hongkong
• [Presentation] In situ imaging and force measurements of an poly(N-isoprop ylacrylamide) surface by atomic force microscopy. (2009)
  • Author(s): Kazuo Umemura, Reiko Kuroda, Akihiko Kikuchi
  • Organizer: Asia Biophysics Symposium
  • Place of Presentation: Hongkong Science and Technology University, Hongkong
• [Presentation] In situ imaging and force measurements of an poly(N-isopropylacrylamide) surface by atomic force microscopy (2008)
  • Author(s): Kazuo Umemura, Reiko Kuroda, Akihiko Kikuchi
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-12-04
• [Presentation] In situ imaging and force measurements of an poly(N-isoprop ylacrylamide) surface by atomic force microscopy. (2008)
  • Author(s): Kazuo Umemura, Reiko Kuroda, Akihiko Kikuchi
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-12-04
• [Remarks]
  • URL: http://www.rs.kagu.tus.ac.jp/biophys/index_jp.html
• [Remarks]
  • URL: http://www.rs.kagu.tus.ac.jp/biophys/index_jp.html