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Elucidation of alternative splicing regulation mechanisms byutilizing in vivo visualization system.

Research Project

Project/Area Number 19510203
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied genomics
Research InstitutionTokyo Medical and Dental University

Principal Investigator

KUROYANAGI Hidehito  Tokyo Medical and Dental University, 大学院疾患生命科学研究部, 准教授 (30323702)

Co-Investigator(Kenkyū-buntansha) TAKEUCHI Akihide  東京医科歯科大学, 難治疾患研究所, 助教 (90436618)
Co-Investigator(Renkei-kenkyūsha) TAKEUCHI Akihide  東京医科歯科大学, 難治疾患研究所, 助教 (90436618)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords選択的スプライシング / 蛍光タンパク質 / レポーター / 線虫 / シス・エレメント / RNA結合タンパク質 / Fox-1 / 可視化 / 遺伝学 / mRNA前駆体
Research Abstract

研究代表者らが開発した、蛍光タンパク質を利用した生体内選択的スプライシング可視化線虫作製技術を応用して、線虫C.elegansのegl-15遺伝子の筋特異的選択的スプライシングおよびlet-2遺伝子の発生段階依存的選択的スプライシングの可視化に成功し、生体における制御機構を明らかにした。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (40 results)

All 2008 2007 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (22 results) Remarks (11 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 2 results)

  • [Journal Article] STAR family RNA-binding protein ASD-2regulates developmental switching of mutually exclusive alternative splicing invivo2008

    • Author(s)
      Ohno G, Hagiwara M, Kuroyanagi H
    • Journal Title

      Genes & Development 22

      Pages: 279-285

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] STAR family RNA-binding protein ASD-2 regulates developmental switching of mutually exclusive alternative splicing in vivo.2008

    • Author(s)
      Ohno G, Hagiwara M, and Kuroyanagi H.
    • Journal Title

      Genes & Development 22

      Pages: 367-374

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fox-1 family and SUP-12coordinately regulate tissue-specificalternative splicing in vivo2007

    • Author(s)
      Kuroyanagi, H, Ohno, G, Mitani, S Hagiwara M
    • Journal Title

      Molecular and Cellular Biology 27

      Pages: 8612-8621

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The SM protein VPS-45 is required for RAB-5-dependent endocytic transport in Caenorhabditis elegans.2007

    • Author(s)
      Gengyo-Ando K, Kuroyanagi H, Kobayashi T, Murate M, Fujimoto K, Okabe S, and Mitani S.
    • Journal Title

      EMBO Report 8

      Pages: 152-157

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Fox-1 family and SUP-12 coordinately regulate tissue-specific2007

    • Author(s)
      Kuroyanagi, H, Ohno, G, Mitani, S and Hagiwara M.
    • Journal Title

      Molecular and Cellular Biology 27

      Pages: 8612-8621

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Alternative splicing reporters offer a path to splicing codes2008

    • Author(s)
      Hidehito Kuroyanagi
    • Organizer
      文部科学省ナショナルバイオリソースプロジェクト線虫シンポジウム
    • Place of Presentation
      東京女子医科大学
    • Year and Date
      2008-11-05
    • Related Report
      2008 Annual Research Report
  • [Presentation] ナショナルバイオリソースプロジェクト(NBRP)線虫、シンポジウム「Nematode : an ideal model organism forfunctional genomics」2008

    • Author(s)
      Hidehito KUROYANAGI
    • Organizer
      Alternativesplicing reporters offer a path to splicing codes
    • Place of Presentation
      東京都
    • Related Report
      2008 Final Research Report
  • [Presentation] A transgenicreporter system reveals expression profilesand regulation mechanisms of alternativesplicing in vivo2008

    • Author(s)
      Hidehito KUROYANAGI
    • Organizer
      UK-Japan Frontiers of Science Symposium
    • Related Report
      2008 Final Research Report
  • [Presentation] Visualization ofalternative splicing patterns in vivo revealsregulation mechanisms2008

    • Author(s)
      Hidehito KUROYANAGI
    • Organizer
      第10回日本RNA学会年会RNAミーティング10周年記念セッション"Ambitious Young Scientists Looking toward the Next Decade"
    • Place of Presentation
      札幌市
    • Related Report
      2008 Final Research Report
  • [Presentation] 線虫unc-32遺伝子の選択的スプライシング制御機構の解析2008

    • Author(s)
      黒柳秀人, 萩原正敏
    • Organizer
      第10回RNAミーティング
    • Place of Presentation
      札幌市
    • Related Report
      2008 Final Research Report
  • [Presentation] Alternative splicing regulation of the C.elegans unc-32 gene2008

    • Author(s)
      Hidehito Kuroyanagi, Masatoshi Hagiwara
    • Organizer
      Gordon ResearchConference, Post-Transcriptional GeneRegulation, The Biology Of, Waterville
    • Place of Presentation
      United States
    • Related Report
      2008 Final Research Report
  • [Presentation] 線虫unc-60遺伝子の筋肉特異的な選択的スプライシング制御機構の解析2008

    • Author(s)
      大野源太, 斧正一郎, 萩原正敏, 黒柳秀人
    • Organizer
      RNAフロンティアミーティング2008
    • Place of Presentation
      京都市
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] 線虫unc-32遺伝子の選択的スプライシング制御機構の解析2008

    • Author(s)
      黒柳秀人, 萩原正敏
    • Organizer
      第10回RNAミーティング
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Related Report
      2008 Annual Research Report
  • [Presentation] Visualization of alternative splicing patterns in vivo reveals regulation mechanisms2008

    • Author(s)
      Hidehito Kuroyanagi
    • Organizer
      第10回RNAミーティング
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Related Report
      2008 Annual Research Report
  • [Presentation] A transgenic reporter system reveals expression profiles and regulation mechanisms of alternative splicing in vivo.2008

    • Author(s)
      Hidehito Kuroyanagi
    • Organizer
      UK-Japan Frontiers of Science Symposium
    • Place of Presentation
      Shonan Village Center in Kanagawa
    • Related Report
      2008 Annual Research Report
  • [Presentation] A transgenic reportersystem reveals expression profiles andregulation mechanisms of alternative splicingin vivo2007

    • Author(s)
      Hidehito Kuroyanagi, Genta Ohno, Masatoshi Hagiwara
    • Organizer
      日本分子生物学会・日本生化学会大会合同大会
    • Place of Presentation
      ワークショップ、横浜市
    • Related Report
      2008 Final Research Report
  • [Presentation] TWO FAMILIES OFRNA-BINDING PROTEINSCO-OPERATIVELY REGULATETISSUE-SPECIFIC ALTERNATIVESPLICING IN C.ELEGANS2007

    • Author(s)
      Hidehito Kuroyanagi, Shohei Mitani, Masatoshi Hagiwara
    • Organizer
      Cold Spring Harbor Laboratory Meeting "EukaryoticmRNA processing"
    • Place of Presentation
      NY, USA
    • Related Report
      2008 Final Research Report
  • [Presentation] COURSE OF PROCESSINGAND SWITCHING MECHANISM OFDEVELOPMENTALLY-REGULATEDALTERANIVE SPLICING IN VIVO2007

    • Author(s)
      Genta Ohno, Masatoshi Hagiwara, Hidehito Kuroyanagi
    • Organizer
      Cold Spring Harbor Laboratory Meeting"Eukaryotic mRNA processing"
    • Place of Presentation
      NY, USA
    • Related Report
      2008 Final Research Report
  • [Presentation] 線虫Fox-1ファミリーRNA 結合タンパク質遺伝子asd-1は選択的スプライシングによる負の自己制御を受ける2007

    • Author(s)
      黒柳秀人, 菊池優理子, 萩原正敏
    • Organizer
      RNA若手研究者の会
    • Place of Presentation
      神戸市
    • Related Report
      2008 Final Research Report
  • [Presentation] 発生段階依存的な相互排他的エクソンの選択性制御機構の解明2007

    • Author(s)
      大野源太, 黒柳秀人, 萩原正敏
    • Organizer
      RNA若手研究者の会
    • Place of Presentation
      神戸市
    • Related Report
      2008 Final Research Report
  • [Presentation] ASD-1/FOX-1とSUP-12は協調して線虫生体内組織特異的選択的スプライシングを制御する2007

    • Author(s)
      黒柳秀人, 萩原正敏
    • Organizer
      第9回RNAミーティング
    • Place of Presentation
      名古屋市
    • Related Report
      2008 Final Research Report
  • [Presentation] 相互排他的エクソンの発生段階依存的選択性制御機構の解明2007

    • Author(s)
      大野源太, 黒柳秀人, 萩原正敏
    • Organizer
      第9回RNA ミーティング
    • Place of Presentation
      名古屋市
    • Related Report
      2008 Final Research Report
  • [Presentation] Hetero-dimerization of ASD-1/FOX-1 and SUP-12 regulates tissue-specific alternativesplicing in vivo2007

    • Author(s)
      Hidehito Kuroyanagi, Genta Ohno, ShoheiMitani, Masatoshi Hagiwara
    • Organizer
      16th International C. elegans Meeting
    • Place of Presentation
      Los Angeles, CA, USA
    • Related Report
      2008 Final Research Report
  • [Presentation] A transgenic reporter systemreveals expression profiles and regulationmechanisms of alternative splicing in vivo2007

    • Author(s)
      Hidehito KUROYANAGI, Masatoshi HAGIWARA
    • Organizer
      第59回日本細胞生物学会大会・第40回日本発生生物学会合同大会
    • Place of Presentation
      ミニ・シンポジウム "Frontiers in RNA biology" 福岡市
    • Related Report
      2008 Final Research Report
  • [Presentation] A transgenic reporter system reveals expression profiles and regulation mechanisms of alternative splicing in vivo.2007

    • Author(s)
      Hidehito Kuroyanagi & Masatoshi Hagiwara
    • Organizer
      第40回日本発生生物学会・第59回日本細胞生物学会 合同大会
    • Place of Presentation
      福岡市
    • Related Report
      2007 Annual Research Report
  • [Presentation] Hetero-dimerization of ASD-1/FOX-1 and SUP-12 regulates tissue-specific alternative splicing in vivo.2007

    • Author(s)
      Hidehito Kuroyanagi, Genta Ohno, Shohei Mitani & Masatoshi Hagiwara
    • Organizer
      16th International C. elegans Meeting
    • Place of Presentation
      米国カリフォルニア州ロサンゼルス
    • Related Report
      2007 Annual Research Report
  • [Presentation] A transgenic reporter system reveals expression profiles and regulation mechanisms of alternative splicing in vivo.2007

    • Author(s)
      Hidehito Kuroyanagi, Genta Ohno & Masatoshi Hagiwara
    • Organizer
      BMB2007(第30回日本分子生物学会・第80回日本生化学会大会 合同大会)
    • Place of Presentation
      横浜市
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.tmd.ac.jp/mri/mri-end/index.html

    • Related Report
      2008 Final Research Report
  • [Remarks] 東京医科歯科大学難治疾患研究所・2008年優秀論文賞(論文(1)、Ohno, et.al.)2009年2月

    • Related Report
      2008 Final Research Report
  • [Remarks] 東京医科歯科大学難治疾患研究所・2007年優秀論文賞(論文(2)、Kuroyanagi, et.al.)2008年2月

    • Related Report
      2008 Final Research Report
  • [Remarks] ゲノムひろば「ゲノム研究勢ぞろい」展示「体の部分によって遺伝子の情報は使われ方が違う!?~光らせて見てみよう~」2008年10月、名古屋市

    • Related Report
      2008 Final Research Report
  • [Remarks] Molecular and Cellular Biology 第28巻第14号表紙2008年7月発刊

    • Related Report
      2008 Final Research Report
  • [Remarks] Nature Reviews Molecular Cell Biology Vol. 9 : p188 RESEARCH HIGHLIGHTS、TECHNOLOGY WATCH「COLOUR-CHANGING WORMS」2008年3月号

    • Related Report
      2008 Final Research Report
  • [Remarks] Nature Reviews Genetics Vol. 9 : p159、RESEARCH HIGHLIGHTS、TECHNOLOGY「Colour-changing worms」 2008 年3 月号Genes & Development 第22巻第3号表紙2008年2月1日発刊

    • Related Report
      2008 Final Research Report
  • [Remarks] Genes & Development 第22巻第3号 : pp279-285、PERSPECTIVE「The search foralternative splicing regulators : new approaches offer a path to a splicing code」2008年2月1日発刊

    • Related Report
      2008 Final Research Report
  • [Remarks] 毎日新聞「たんぱく作り分け 遺伝子の暗号特定東京医科歯科大が手法開発」2008年1月30日朝刊2面掲載

    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.tmd.ac.jp/cmn/soumu/kouhou/news20080125.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.tmd.ac.jp/mri/mri-end/research_pg2.html

    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] A Transgenic Reporter System ThatReveals Expression Profiles and RegulationMechanisms Of Alternative Splicing In LivingOrganisms2007

    • Inventor(s)
      Kuroyanagi, H.Hagiwara, M
    • Industrial Property Rights Holder
      株式会社キノファーマ
    • Filing Date
      2007-09-26
    • Related Report
      2008 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 米国特許2007

    • Inventor(s)
      Kuroyanagi, H. & Hagiwara, M.
    • Industrial Property Rights Holder
      株式会社キノファーマ
    • Filing Date
      2007-09-26
    • Related Report
      2007 Annual Research Report
    • Overseas

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Published: 2007-04-01   Modified: 2016-04-21  

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