nvestigation of the molecular mechanism of sulfur assimilation and amino acid accumulation in plants

• Project/Area Number: 19510212
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Tokushima Bunri University
• Principal Investigator: NOJI Masaaki Tokushima Bunri University, 薬学部, 准教授 (80271534)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 生合成 / 硫黄同化 / システイン生合成 / システイン合成酵素

Research Abstract

植物の硫黄同化経路の特徴の一つは、各酵素反応ステップに複数の酵素アイソザイムが関与している点であるが、各アイソフォームに特有な機能や役割については、その詳細は明らかとなっていなかった。そこで本研究では、シロイヌナズナを用いて硫黄同化に関与する各アイソザイムの役割をトランスクリプトミクス、プロテオミクス、メタボロミクスというポストゲノム戦略によって解析し、硫黄の吸収・同化における個々のアイソザイムや細胞機能、分担機構を解明した。

Research Products

• [Journal Article] Disruption of adenosine-5'-phosphosulfate kinase in Arabidopsis reduces levels of sulfate secondary metabolites (2009)
  • Author(s): Mugford SG, Yoshimoto N, Reichelt M, Wirtz M, Hill L, Mugford ST, Nakazato Y, Noji M, Takahashi H, Kramell R, Gigolashvili T, Flugge UI, Wasternack C, Gershenzon J, Hell R, Saito K, Kopriva S
  • Journal Title: Plant Cell 3 Pages: 910-927
  • Peer Reviewed
• [Journal Article] Disruption of Adenosine-5'-Phosphosulfate Kinase in Arabidopsis Reduces Levels of Sulfated Secondary Metabolites (2009)
  • Author(s): Sarah G. Mugford
  • Journal Title: Plant Cell 21 Pages: 910-927
  • Peer Reviewed
• [Journal Article] Comparative genomics and reverse genetics analysis reveal indispensable functions of the serine acetyltransferase gene family in Arabidopsis (2008)
  • Author(s): Watanabe M, Mochida K, Kato T, Tabata S, Yoshimoto N, Noji M, Saito K
  • Journal Title: Plant Cell 20 Pages: 2484-2496
  • Peer Reviewed
• [Journal Article] Efficient and high-throughput vector construction and Agrobacterium-mediated transformation of Arabidopsis thaliana suspension-cultured cells for functional genomics (2008)
  • Author(s): Ogawa Y, Dansako T, Yono K, Sakurai N, Suzuki H, Aoki K, Noji M, Saito K, Shibata D
  • Journal Title: Plant Cell Physiol. 49 Pages: 242-250
  • NAID: 10027328964
  • Peer Reviewed
• [Journal Article] Physiological roles of the beta-substituted alanine synthase gene family in Arabidopsis (2008)
  • Author(s): Watanabe M, Kusano M, Oikawa A, Fukushima A, Noji M, Saito K
  • Journal Title: Plant Physiol. 149 Pages: 310-320
  • Peer Reviewed
• [Journal Article] Comparative genomics and reverse genetics analysis reveal Indispensable function of the serine acetyltransferase gene family in Arabidopsis (2008)
  • Author(s): Mutsumi Watanabe
  • Journal Title: Plant Cell 20 Pages: 2484-2496
  • Peer Reviewed
• [Journal Article] Physiological roles of the beta-substituted alanine synthase gene family in Arabidopsis (2008)
  • Author(s): Mutsumi Watanabe
  • Journal Title: Plant Physiology 146 Pages: 310-320
  • Peer Reviewed
• [Journal Article] Efficient and high-throughput vector construction and Agrobacterium-mediated transformation of Arabidopsis thaliana suspension-cultured cells for functional genomics (2008)
  • Author(s): Yoichi Ogawa
  • Journal Title: Plant & Cell Physiology 49 Pages: 242-250
  • Peer Reviewed
• [Presentation] シロイヌナズナの硫黄同化系酵素ATPスルフリラーゼの機能解析 (2009)
  • Author(s): 吉本尚子
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-22
• [Presentation] シロイヌナズナの硫黄代謝に関わるAPSキナーゼ群の機能解析 (2008)
  • Author(s): 吉本尚子,中里好美,嶋聡子,高橋秀樹,野路征昭,斉藤和季
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
• [Presentation] シロイヌナズナ変異株を用いたセリンアセチル転移酵素遺伝子群のシステイン生合成系における機能分担の解析 (2007)
  • Author(s): 渡辺 むつみ
  • Organizer: 日本植物細胞分子生物学会大会・シンポジウム
  • Place of Presentation: 千葉大学工学部
  • Year and Date: 2007-08-08
• [Presentation] シロイヌナズナ変異株を用いたセリンアセチル転移酵素遺伝子群のシステイン生合成系における機能分担の解析 (2007)
  • Author(s): 渡辺むつみ,野路征昭,加藤友彦,田畑哲之,斉藤和季
  • Organizer: 第25回日本植物細胞分子生物学会大会・シンポジウム
  • Place of Presentation: 千葉
• [Presentation] 硫黄代謝に関与するシロイヌナズナのAPSキナーゼ遺伝子群の機能解析 (2007)
  • Author(s): 中里好美,嶋聡子,野路征昭,斉藤和季
  • Organizer: 第25回日本植物細胞分子生物学会大会・シンポジウム
  • Place of Presentation: 千葉