The structural domains of Chondromodulin-I, an angiogenesis inhibitor, and their contribution to its bioactivity

• Project/Area Number: 19510217
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Kyoto University
• Principal Investigator: HIRAKI Yuji Kyoto University, 再生医科学研究所, 教授 (40144498)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 活性発現の分子機構 / 蛋白質 / 発生・分化 / 血管侵入抵抗性 / 癌 / chondromodulin-I

Research Abstract

Chondromodulin-Iは無血管軟骨特異的に発現し、その細胞外基質に蓄積する生理的血管新生抑制因子である。血管新生促進因子と比較して、血管新生抑制因子の血管内皮細胞に対する作用機序は、十分に解明されていない。そこで、本研究では、まず、無血清条件下にヒトChondromodulin-Iの大量発現を確立すると同時に迅速簡便精製を行い、高活性の組換え体を調製する。さらに、欠失を含む種々の変異組換えChondromodulin-Iタンパク質を発現させる事により、Chondromoduin-Iの分子特性と活性ドメインの構造を明らかにすることに成功した。

Research Products

• [Journal Article] Chondromodulin-I and Tenomodulin are differentially expressed in the avascular mesenchyme during mouse and chick development (2008)
  • Author(s): C. Shukunami, A. Takimoto, S. Miura, Y. Hiraki
  • Journal Title: Cell Tissue Res. 332 Pages: 111-122
  • Peer Reviewed
• [Journal Article] Altered fracture callus formation in chondromodulin-I deficient mice (2008)
  • Author(s): K. Yukata, Y. Matsui, C. Shukunami, A. Takimoto, T. Goto, Y. Nishizaki, Y. Nakamichi, T. Kubo, T. Sano, S. Kato, Y. Hiraki, N. Yasui
  • Journal Title: Bone 43 Pages: 1047-1056
  • Peer Reviewed
• [Journal Article] The chondrogenic repair response of undifferentiated mesenchymal cells in rat full-thickness articular cartilage defects (2008)
  • Author(s): Y. Anraku, H. Mizuta, S. Kudo, E. Nakamura, K. Takagi, Y. Hiraki
  • Journal Title: Osteoarthritis Cartilage 16 Pages: 961-964
  • Peer Reviewed
• [Journal Article] Altered fracture callus formation in chondromodulin-I deficient mice. (2008)
  • Author(s): Yukata K, 他
  • Journal Title: Bone 43 Pages: 1047-1056
  • Peer Reviewed
• [Journal Article] Analyses of early events during chondrogenic repair in rat full-thickness articular cartilage defects. (2008)
  • Author(s): Anrakum Y, 他
  • Journal Title: J. Bone Min. Metab. 27 Pages: 272-286
  • Peer Reviewed
• [Journal Article] Chondromodulin-I and Tenomodulin are differentially expressed in the avascular mesenchyme during mouse and chick development. (2008)
  • Author(s): Shukunami C, 他
  • Journal Title: Cell Tissue Res. 332 Pages: 111-122
  • Peer Reviewed
• [Journal Article] the Negative Control of Angiogenesis in Connective Tissue. Curr. Pharm (2007)
  • Author(s): C. Shukunami, Y. Hiraki Chondromodulin-I and Tenomodulin
  • Journal Title: Design 13 Pages: 2101-2112
  • Peer Reviewed
• [Journal Article] Chondromodulin-I and Tenomodulin: the Negative Control of Angiogenesis in Connective Tissue. (2007)
  • Author(s): Shukunami C, Hiraki Y
  • Journal Title: Curr Pharm Design 13 Pages: 2101-2112
  • Peer Reviewed
• [Presentation] 結合組織の血管侵入抵抗性と軟骨形成 (2008)
  • Author(s): 開祐司
  • Organizer: 第23回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-10-23
• [Presentation] 軟骨形成・維持とECM環境 (2008)
  • Author(s): 開祐司
  • Organizer: 第29回日本炎症・再生医学会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-09
• [Presentation] Chondromodulin-Iの血管内皮細胞に対する遊走阻害活性 (2007)
  • Author(s): 三浦重徳、宿南知佐、開祐司
  • Organizer: 第8回運動器科学研究会
  • Place of Presentation: 徳島
  • Year and Date: 2007-08-24
• [Presentation] A potential role of decidual chondromodulin-I in the control of cell migration and invasion of trophoblast giant cells (2007)
  • Author(s): S. Miura, C. Shukunami, Y. Nishizaki, Y. Hiraki
  • Organizer: The 21st Century COE Symposium for Integration of Transplantation Therapy and Regenerative Medicine
  • Place of Presentation: 京都
  • Year and Date: 2007-06-29
• [Presentation] A potential role of decidual chondromodulin-I in the control of cell migration and invasion of trophoblast giant cells. (2007)
  • Author(s): Shigenori Miura, 他
  • Organizer: The 21st Century COE Symposium for Integration of Transplantation Therapy and Regenerative Medicine
  • Place of Presentation: 京都
  • Year and Date: 2007-06-29
• [Remarks]
  • URL: http://www.frontier.kyoto-u.ac.jp/te01