Chemical Synthesis and Highly Sensitive Analysis of Abnormal Δ^5-Bile Acid Multi Conjugates Present in Patients with Progressive Familial Intrahepatic Cholestasis
| Project/Area Number |
19510223
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Living organism molecular science
|
|---|
| Research Institution | Nihon University |
|---|
Principal Investigator |
IIDA Takashi Nihon University, 文理学部, 教授 (60060125)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAKIYAMA Genta 日本大学, 文理学部, 助手 (70453868)
|
|---|
| Project Period (FY) |
2007 – 2008
|
| Project Status |
Completed (Fiscal Year 2008)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 生体分子 / PFIC症 / 二次異常代謝産物 / ステロイド多重抱合体 / LC-MS |
|---|
| Research Abstract |
本研究課題は先天性胆汁酸代謝異常症患者尿中に顕著かつ、特異的に排泄される3β-hydroxy-Δ^5構造をもつ多重抱合型胆汁酸を取り上げ、有機合成化学的手法を用いて標品の高効率な化学合成法を確立すると共に、液体クロマトグラフィー-質量スペクトル(LC-MS)法を駆使し、生体試料中に存在する3β-hydroxy-Δ^5胆汁酸多重抱合体を疾患マーカーとする高感度で信頼度の高い直接一斉分析法を確立する。
|
Report
(3 results)
Research Products
(39 results)
-
[Journal Article] Identification of a new C_<27> bile acid from Red-winged tinamou (Rhynchotus rufescens) : (25R)-1β, 3α, 7α-trihydroxy-5β-cholestan-27-oic acid2009
Author(s)
Hagey L. R, Kakiyama G, Muto A, Iida T, Mushiake K, Goto T, Mano N, Goto J, Oliveira C. A, Hofmann A. F
-
Journal Title
J. Lipid Res 50
Pages: 651-657
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] Isolation and Chemical Synthesis of a Major, Novel Biliary Bile Acid in the Common Wombat (Vombatus ursinus) : 15α-hydroxylithocholic acid2007
Author(s)
Kakiyama G, Tamegai H, Iida T, Mitamura K, Ikegawa S, Goto T, Mano N, Goto J, Holz P, Hagey L. R, Hofmann A. F
-
Journal Title
J. Lipid Res 48
Pages: 2682-2692
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
