Development of labeling reagents for quantitative RNA detection
Project/Area Number |
19510230
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Living organism molecular science
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Research Institution | National Institute of Advanced Industrial Science and Technology |
Principal Investigator |
(KOMATSU Yasuo National Institute of Advanced Industrial Science and Technology, ゲノムファクトリー研究部門, 研究グループ長 (30271670)
|
Project Period (FY) |
2007 – 2008
|
Project Status |
Completed (Fiscal Year 2008)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | RNA / 検出 / 定量 / 遺伝子 / DNA |
Research Abstract |
本研究ではRNAの高感度、定量検出を目的に、酸化したRNAの3'末端に高い反応効率で一分子の標識試薬のみが結合する新規な試薬の合成を行った。この新規標識試薬は、核酸の塩基とリン酸部に高い親和性を有するように設計、合成したところ、RNAに対して高効率で反応することが明らかとなった。さらに、この化合物にビオチンを導入した化合物も合成し、RNAの検出が可能であることも明らかにした。
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Report
(3 results)
Research Products
(21 results)
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[Journal Article] Construction of an aminooxy derivative for RNA and DNA labeling2008
Author(s)
Komatsu, Y., Kojima, N., Takebayashi, T., Mikami, A., Sugino, M. and Ohtsuka,E
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Journal Title
Nucleic Acids Symp. Ser. (Oxf) 52
Pages: 393-394
Related Report
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[Journal Article] Comparison of the chemical properties of a novel amino-linker with various amino modifications2008
Author(s)
Kojima, N., Takebayashi, T., Mikami, A., Sugino, M., Ohtsuka, E. and Komatsu,Y
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Journal Title
Nucleic Acids Symp. Ser. (Oxf) 52
Pages: 463-464
Related Report
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[Journal Article] Construction of time-lapse scanning electrochemical microscopy with temperature control and its application to evaluate the preservation effects of antifreeze proteins on living cells2008
Author(s)
Hirano, Y., Nishimiya, Y., Kowata, K., Mizutani, F., Tsuda, S. and Komatsu, Y
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Journal Title
Anal. Chem 80
Pages: 9349-9354
Related Report
Peer Reviewed
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[Journal Article] Hypothermic preservation effect on mammalian cells of type III antifreeze proteins from notched-fin eelpout2008
Author(s)
Hirano, Y., Nishimiya, Y., Matsumoto, S., Matsushita, M., Todo, S., Miura, A., Komatsu, Y. and Tsuda, S
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Journal Title
Cryobiology 57
Pages: 46-51
Related Report
Peer Reviewed
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[Journal Article] Novel amino linkers enablingefficient labeling and convenient purification of amino-modified oligonucleotides2008
Author(s)
Komatsu, Y., Kojima, N., Sugino, M., Mikami, A., Nonaka, K., Fujinawa, Y., Sugimoto, T., Sato, K., Matsubara, K. and Ohtsuka, E
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Journal Title
Bioorg. Med. Chem 16
Pages: 941-949
Related Report
Peer Reviewed
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[Journal Article] Osteopontin small interfering RNA protects mice from fulminant hepatitis2007
Author(s)
Saito, Y., Kon, S., Fujiwara, Y., Nakayama, Y., Kurotaki, D., Fukuda, N., Kimura, C., Kanayama, M., Ito, K., Diao, H., Matsui, Y., Komatsu, Y., Ohtsuka, E. and Uede, T
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Journal Title
Hum. Gene Ther 18
Pages: 1205-1214
Related Report
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