Establishment of a Fast Diagnosis Technique for Toxicity of a Chemical to Cells by Deflection of a Probe Beam

• Project/Area Number: 19550082
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: University of Fukui
• Principal Investigator: WU Xing-zheng University of Fukui, 大学院・工学研究科, 准教授 (70234961)
• Co-Investigator(Kenkyū-buntansha): TERADA Satoshi 福井大学, 大学院・工学研究科, 准教授 (60311685)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 細胞 / 計測 / 化学物質の毒性 / プローブ光 / 偏向 / 毒性 / 光偏向

Research Abstract

化学物質の生体への毒性は今まで人間への被害報告で分かったものが多いが、現在動物実験あるいはin-vitro細胞培養実験で研究されるようになった。一方、ある化学物質が細胞に対して強い毒性を示す場合、その化学物質の存在により生存細胞が死滅したりするので、細胞膜近傍を通すプローブ光の偏向信号が大きく変化する。本研究はこの発想に基づいて、プローブ光一本で化学物質の細胞への毒性を判定する方法を開発した。ヒト肝臓由来のHepG2細胞に対して330~370nmの紫外線が強い殺傷力を持つことを明らかにした。また、アルコール及び過酸化水素の毒性も検討した。

Research Products

• [Journal Article] Study of Protein-Protein Binding Reaction by Whole Column Fluorescence Imaged Capillary Isoelectric Focusing (2009)
  • Author(s): Xing-Zheng Wu, Shinya Asai, Yoshie Yamaguchi
  • Journal Title: Electrophoresis (in press)
  • Peer Reviewed
• [Journal Article] Chemiluminescence from Luminol Solution after Illumination of a 355 nm-Pulse Laser (2009)
  • Author(s): Lingyue Min, Xing-Zheng Wu
  • Journal Title: Luminescence (in press)
• [Journal Article] Study of Protein-Protein Binding Reaction by Whole Column Fluorescence Imaged Capillary Isoelectric Focusing (2009)
  • Author(s): Xing-Zheng Wu, Shinya Asai, Yoshie Yamaguchi
  • Journal Title: Electrophoresis (In press)
  • Peer Reviewed
• [Journal Article] Chemiluminescence from Luminol Solution after Illumination of a 355 nm-Pulse Laser (2009)
  • Author(s): Lingyue Min, Xing-Zheng Wu
  • Journal Title: Luminescence (In press)
  • Peer Reviewed
• [Journal Article] キャピラリー電気泳動分析の新規キャピラリー内濃縮法の開発(総合論文) (2008)
  • Author(s): 呉行正
  • Journal Title: 分析化学 57 Pages: 77-90
  • Peer Reviewed
• [Journal Article] キャピラリー電気泳動分析の新規キャピラリー内濃縮法の開発 (2008)
  • Author(s): 呉行正
  • Journal Title: 分析化学 57 Pages: 77-90
  • NAID: 110006596188
  • Peer Reviewed
• [Journal Article] キャピラリー電気泳動分析の新規キャピラリー内濃縮法の開発 (2008)
  • Author(s): 呉 行正
  • Journal Title: 分析化学 57 Pages: 77-90
  • NAID: 110006596188
  • Peer Reviewed
• [Journal Article] Optical Beam Deflection Approach for Studying Ion-exchange Reactions Occurring at a Single Ion-exchange Resin Particle (2007)
  • Author(s): Xing-Zheng Wu, Yumiko Tsuji
  • Journal Title: Norio Teramae, Reactive and Functional Polymers 67 Pages: 113-119
  • Peer Reviewed
• [Journal Article] Hazard Identification on a Single Cell Level Using a Laser Beam (2007)
  • Author(s): Xing-Zheng Wu, Tomohisa Kato, Yumiko Tsuji, Satoshi Terada
  • Journal Title: Anal. Chem. Insight 2 Pages: 119-124
  • Peer Reviewed
• [Journal Article] A Simple Transmitted Interference Method for Nano-volume Detection, Cong Dai (2007)
  • Author(s): Xing-Zheng Wu
  • Journal Title: Chem. Lett 36 Pages: 1334-1335
  • Peer Reviewed
• [Journal Article] On-capillary Chemiluminescence Detection for Capillary Electrophoresis with a Single Capillary, Cheng-Jie ZHANG (2007)
  • Author(s): Xing-Zheng WU
  • Journal Title: Anal. Sci 23 Pages: 743-746
  • Peer Reviewed
• [Journal Article] Capillary Electrophoresis with In-capillary Solid Phase Extraction Sample Clean-up (2007)
  • Author(s): Luo-Hong Zhang, Xing-Zheng Wu
  • Journal Title: Anal. Chem 79 Pages: 2562-2579
  • Peer Reviewed
• [Journal Article] Time-resolved Chemiluminescent Study of the TiO2 Photocatalytic Reaction and Its Induced Active Oxygen Species, Lingyue Min (2007)
  • Author(s): Xing-Zheng Wu, Tetsuya Shimada, Haruo Inoue
  • Journal Title: Luminescence 22(2) Pages: 105-112
  • Peer Reviewed
• [Journal Article] Optical Beam Deflection Approach for Studying Ion-exchange Reactions Occurring at a Single Ion-exchange Resin Particle (2007)
  • Author(s): Xing-Zheng Wu, Yumiko Tsuji, Norio Teramae
  • Journal Title: Reactive and Functional Polymers 67 Pages: 113-119
  • Peer Reviewed
• [Journal Article] Capillary Electrophoresis with In-capillary Solid Phase Extraction Sample Clean-up (2007)
  • Author(s): Luo-Hong Zhang, Xing-Zheng Wu
  • Journal Title: Anal. Chem. 79 Pages: 2562-2579
  • Peer Reviewed
• [Journal Article] Time-resolved Chemiluminescent Study of the TiO_2 Photocatalytic Reaction and Its Induced Active Oxygen Species (2007)
  • Author(s): Lingyue Min, Xing-Zheng Wu, Tetsuya Shimada, Haruo Inoue
  • Journal Title: Luminescence 22 Pages: 105-112
  • Peer Reviewed
• [Journal Article] A Simple Transmitted Interference Method for Nano-volume Detection (2007)
  • Author(s): Cong Dai and Xing-Zheng Wu
  • Journal Title: Chem. Lett. 36 Pages: 1334-1335
  • Peer Reviewed
• [Journal Article] On-capillary Chemiluminescence Detection for Capillary Electrophoresis with a Single Capillary (2007)
  • Author(s): Cheng-Jie ZHANG and Xing-Zheng WU
  • Journal Title: Anal. Sci. 23 Pages: 743-746
  • Peer Reviewed
• [Presentation] 化学物質の細胞への毒性の光偏向判定法 (2008)
  • Author(s): 加藤智久, 寺田聡, 呉行正
  • Organizer: 第69回日本分析化学討論会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-05-20
• [Presentation] Noninvasive Diagnosis of a Single Cell with a Laser Beam (2008)
  • Author(s): X.-Z.Wu, T. Kato, S.Terada
  • Organizer: Pittcon 2008
  • Place of Presentation: New Orleans(USA)
  • Year and Date: 2008-03-04
• [Presentation] Study of Protein-Protein Interaction by Capillary Electrophoresis and Whole Column Imaged Capillary Isoelectric Focusing (2007)
  • Author(s): X.-Z.Wu, S. Asai
  • Organizer: Asia-Pacific International Symposium on Microscale Separation and Analysis
  • Place of Presentation: シンガポール
  • Year and Date: 2007-12-17
• [Presentation] Study of Protein-Protein Interaction by Capillary Electrophoresis and Whole Column Imaged Capillary Isoelectric Focusing (2007)
  • Author(s): X. -Z. Wu, S. Asai
  • Organizer: Asia-Pacific International Symposium on MIcroscale Separation and Analysis
  • Place of Presentation: シンガポール
  • Year and Date: 2007-12-17
• [Presentation] 単一細胞の非侵襲的なビーム偏向測定及び化学試薬の細胞への毒性の研究 (2007)
  • Author(s): 加藤智久, 寺田聡, 呉行正
  • Organizer: 日本分析化学会第56年会
  • Place of Presentation: 徳島
  • Year and Date: 2007-09-10
• [Presentation] キャピラリー干渉検出法の開発 (2007)
  • Author(s): 戴ちょん、呉行正
  • Organizer: 日本分析化学会第56年
  • Place of Presentation: 徳島
  • Year and Date: 2007-09-10
• [Presentation] In-Capillary固相抽出-キャピラリー電気泳動法の開発 (2007)
  • Author(s): 張洛紅, 呉行正
  • Organizer: 日本分析化学会第56年会
  • Place of Presentation: 徳島
  • Year and Date: 2007-09-10
• [Presentation] 全カラムイメージングキャピラリー等電点電気泳動法によるタンパク質とナノ粒子の相互作用の研究 (2007)
  • Author(s): 浅井真哉, 山啓介, 呉行正
  • Organizer: 日本分析化学会第56年会
  • Place of Presentation: 徳島
  • Year and Date: 2007-09-10
• [Presentation] キャピラリー干渉検出法の開発 (2007)
  • Author(s): 戴 ちょん、呉 行正
  • Organizer: 日本分析化学会第56年会
  • Place of Presentation: 徳島
  • Year and Date: 2007-09-10