| Project/Area Number |
19550088
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
IKEGAMI Tohru Kyoto Institute of Technology, 工芸科学研究科, 准教授 (20301252)
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| Project Period (FY) |
2007 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | HPLC / カラム / 精密分離 / 糖鎖 / 高速分離 / シリカモノリス / 親水性相互作用型クロマトグラフィー / 重合修飾法 / 未修飾糖 / HILIC-ESI-MS |
|---|
| Research Abstract |
Monolithic silica columns in fused silica capillaries of 100-200mm diameters were functionalized by on-column radical polymerization of methacrylates possessing various hydrophilic/ion-exchange functions. These columns with hydrophilic, weak cation exchange, and weak anion exchange functions, were provided for the separation of mono- and oligosaccharides and their derivatives using microHPLC systems to evaluate their separation efficiencies. They could provide higher separation efficiencies than commercial particle-packed columns in terms of plate numbers of the HPLC system, and separation speed, in the separation of saccharides, nucleosides, and nucleotides.
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