| Project/Area Number |
19550098
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Analytical chemistry
|
|---|
| Research Institution | Tokyo University of Technology |
|---|
Principal Investigator |
YANO Kazuyoshi Tokyo University of Technology, 応用生物学部, 准教授 (40262109)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
秋元 卓央 東京工科大学, バイオニクス学部, 講師 (90367194)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
AKIMOTO Takuo 東京工科大学, 応用生物学部, 講師 (90367194)
|
|---|
| Project Period (FY) |
2007 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | プロテオミクス / プラズマ重合 / 分析化学 / 薄膜 / 蛍光 |
|---|
| Research Abstract |
Fluorescence enhancement structure was built up on the glass substrate. First, the Ag film was deposited on the slide glass. The hexamethyldisiloxane plasma-polymerized film (PPF) was subsequently deposited as a dielectric film with various film thickness. The fluorophore-labeled protein was spotted, and the fluorescence intensity from the substrate was measured. As a result, the fluorescence intensity could be enhanced about approximately 13-fold when the thickness of PPF was approximately 56nm, suggesting the future possibility of highly sensitive immunoassay.
|
