Development of a highly sensitive protein detection method using substrates with nano-scale strcture.
Project/Area Number |
19550098
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Analytical chemistry
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Research Institution | Tokyo University of Technology |
Principal Investigator |
YANO Kazuyoshi Tokyo University of Technology, 応用生物学部, 准教授 (40262109)
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Co-Investigator(Kenkyū-buntansha) |
秋元 卓央 東京工科大学, バイオニクス学部, 講師 (90367194)
|
Co-Investigator(Renkei-kenkyūsha) |
AKIMOTO Takuo 東京工科大学, 応用生物学部, 講師 (90367194)
|
Project Period (FY) |
2007 – 2009
|
Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | プロテオミクス / プラズマ重合 / 分析化学 / 薄膜 / 蛍光 |
Research Abstract |
Fluorescence enhancement structure was built up on the glass substrate. First, the Ag film was deposited on the slide glass. The hexamethyldisiloxane plasma-polymerized film (PPF) was subsequently deposited as a dielectric film with various film thickness. The fluorophore-labeled protein was spotted, and the fluorescence intensity from the substrate was measured. As a result, the fluorescence intensity could be enhanced about approximately 13-fold when the thickness of PPF was approximately 56nm, suggesting the future possibility of highly sensitive immunoassay.
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Report
(4 results)
Research Products
(7 results)
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[Journal Article] Fully Automated Two-Dimensional Electrophoresis System for High-Throughput Protein Analysis2007
Author(s)
A. Hiratsuka, H. Kinoshita, Y. Maruo, K. Takahashi, S. Akutsu, C. Hayashida, K. Sakairi, K. Usui, K. Shiseki, H. Inamochi, Y. Nakada, K. Yodoya, I. Namatame, Y. Unuma, M. Nakamura, K. Ueyama, Y. Ishii, K. Yano, K. Yokoyama
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Journal Title
Analytical Chemistry 79
Pages: 5730-5739
Related Report
Peer Reviewed
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