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Real time analysis of interactions between biopolyester and its degrading enzyme

Research Project

Project/Area Number 19550156
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental chemistry
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

YAMASHITA Koichi  The Institute of Physical and Chemical Research, 化学分析チーム, 専任研究員 (90174672)

Co-Investigator(Kenkyū-buntansha) HIRAISHI Tomohiro  独立行政法人理化学研究所, 前田バイオ工学研究室, 専任研究員 (20321804)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords環境材料 / 微生物 / 生分解性ポリエステル / QCM / 分解菌 / 大腸菌 / 吸着
Research Abstract

バイオポリエステルと分解菌の相互作用を解析するために,培養槽と水晶発振子マイクロバランス(QCM)を組み合わせた循環システムを開発したが,気泡の混入と培地の吸着のために安定的なデータを得ることは困難であった.また,分解菌モデルとして菌表面に分解酵素を発現させた大腸菌を調製してバイオポリエステルとの相互作用を解析したところ,菌吸着によるフィルム重量の増加とフィルム表面の粘弾性変化を同時観測することに成功した.

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (21 results)

All 2009 2008 2007

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (12 results)

  • [Journal Article] Cloning of poly(aspartic acid) (PAA) hydrolase-1 gene from Pedobacter sp. KP-2 and hydrolysis of thermally synthesized PAA by its gene product2009

    • Author(s)
      Tomohiro Hiraishi, Eriko Masuda, Madoka Nagata, Naoki Kanayama, Yoshiharu Doi, Hideki Abe, and Mizuo Maeda
    • Journal Title

      Macromol. Biosci. 9

      Pages: 10-19

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cloning of poly(aspartic acid) (PAA) hydrolase-1 gene from Pedobacter sp. KP-2 and hydrolysis of thermally synthesized PAA by its gene product2009

    • Author(s)
      Tomohiro Hiraishi
    • Journal Title

      Macromol. Biosci. 9

      Pages: 9-19

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural Effects of Terminal Groups on Nonenzymatic and Enzymatic Degradations of End-Capped Poly(L-lactide)2008

    • Author(s)
      Kenji Kurokawa, Koichi Yamashita, Yoshiharu Doi and Hideki Abe
    • Journal Title

      Biomacromolecules 9

      Pages: 1071-1078

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Adsorption characteristics of P(3HB) depolymerase as evaluated by surface plasmon resonance and atomic force microscopy2008

    • Author(s)
      Nobuhiko Matsumoto, Masahiro Fujita, Tomohiro Hiraishi, Hideki Abe, and Mizuo Maeda
    • Journal Title

      Biomacromolecules 9

      Pages: 3201-3207

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural Effects of Terminal Groups on Nonenzymatic and Enzymatic Degradations of End-Capped Poly (L-lactide)2008

    • Author(s)
      Kenji Kurokawa
    • Journal Title

      Biomacromolecules 9

      Pages: 1071-1078

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adsorption characteristics of P(3HB) depolymerase as evaluated by surface plasmon resonance and atomic force microscopy2008

    • Author(s)
      Nobuhiko Matsumoto
    • Journal Title

      Biomacromolecules 9

      Pages: 3201-3207

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural Effects of Terminal Groups on Nonenzymatic and Enzymatic Degradations of End-Capped Poly(L-lactide)2008

    • Author(s)
      Kenji Kurokawa
    • Journal Title

      Biomacromolecules 9(in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adsorption and Hydrolysis Reactions of Poly(hydroxybutyric acid) Depolymerases Secreted from Ralstonia pickettii T1 and Penicillium funiculosum onto Poly[(R)-3-hydroxybutyric acid]2007

    • Author(s)
      Keiji Numata, Koichi Yamashita, Masahiro Fujita, Takeharu Tsuge, Ken-ichi Kasuya, Tadahisa Iwata, Yoshiharu Doi, and Hideki Abe
    • Journal Title

      Biomacromolecules 8

      Pages: 2276-2281

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Adsorption and Hydrolysis Reactions of Poly(hydroxybutyric acid)Depolymerases Secreted from Ralstonia pickettii T1 and Penicillium funiculosum onto Poly[(R)-3-hydroxybutyric acid]2007

    • Author(s)
      Keiji Numata
    • Journal Title

      Biomacromolecules 8

      Pages: 2276-2281

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Kinetics of Enzymatic Degradation of Poly(L-lactide) by commercially-available proteases2008

    • Author(s)
      Koichi Yamashita
    • Organizer
      International Symposium on Biological Polyesters
    • Place of Presentation
      Auckland, New Zealand
    • Year and Date
      2008-11-26
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Characteristics of poly(aspartic acid) hydrolase and its application2008

    • Author(s)
      Tomohiro Hiraishi
    • Organizer
      International Symposium on Biological Polyesters
    • Place of Presentation
      Auckland, New Zealand
    • Year and Date
      2008-11-26
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] 市販プロテアーゼによるポリ(L-乳酸)の酵素分解2008

    • Author(s)
      山下宏一
    • Organizer
      第57回高分子討論会
    • Place of Presentation
      大阪市
    • Year and Date
      2008-09-26
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] PHB分解酵素吸着部位におけるアミノ酸置換が与えるPHB酵素分解挙動の変化2008

    • Author(s)
      小宮直也・平石知裕・前田瑞夫
    • Organizer
      第57回高分子討論会
    • Place of Presentation
      大阪市
    • Year and Date
      2008-09-26
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] 分子鎖末端修飾によるポリ乳酸の表面特性改変と高機能化2008

    • Author(s)
      阿部英喜・黒川賢志・山下宏一・土肥義治
    • Organizer
      第57回高分子討論会
    • Place of Presentation
      大阪市
    • Year and Date
      2008-09-26
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] ポリアスパラギン酸分解酵素の性質とそれを用いたポリアミノ酸合成2008

    • Author(s)
      平石知裕・舛田エリ子・阿部英喜・前田瑞夫
    • Organizer
      第57回高分子学会年次大会
    • Place of Presentation
      横浜市
    • Year and Date
      2008-05-29
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] ポリ乳酸表面へのタンパク質吸着の制御2008

    • Author(s)
      山下宏一・黒川賢志・阿部英喜
    • Organizer
      第57回高分子学会年次大会
    • Place of Presentation
      横浜市
    • Year and Date
      2008-05-28
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] ポリ乳酸の加水分解性に及ぼす末端官能基の構造効果2008

    • Author(s)
      阿部英喜・黒川賢志・山下宏一・土肥義治
    • Organizer
      第57回高分子学会年次大会
    • Place of Presentation
      横浜市
    • Year and Date
      2008-05-28
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Enzymatic Degradation of Poly(L-lactide) by proteases2007

    • Author(s)
      Koichi Yamashita
    • Organizer
      International Symposium on Polymers and The Environment
    • Place of Presentation
      Vancouver, Washington, USA
    • Year and Date
      2007-10-19
    • Related Report
      2008 Final Research Report
  • [Presentation] Enzymatic Degradation of Poly(L-lactide)by proteases2007

    • Author(s)
      Koichi Yamashita
    • Organizer
      International Symposium on Polymers and The Environment
    • Place of Presentation
      Vancouver,Washington,USA
    • Year and Date
      2007-10-19
    • Related Report
      2007 Annual Research Report
  • [Presentation] 生分解性ポリマー表面における酵素反応の解析2007

    • Author(s)
      山下宏一
    • Organizer
      第1回日本化学会関東支部大会
    • Place of Presentation
      八王子市
    • Year and Date
      2007-09-27
    • Related Report
      2008 Final Research Report
  • [Presentation] ポリ乳酸のプロテアーゼによる分解機構2007

    • Author(s)
      山下宏一・城座彩子
    • Organizer
      第56回高分子討論会
    • Place of Presentation
      名古屋市
    • Year and Date
      2007-09-20
    • Related Report
      2008 Final Research Report 2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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