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Development of fluorescent probes based on calixarene-coated water soluble quantum dots

Research Project

Project/Area Number 19550157
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemistry related to living body
Research InstitutionOsaka University

Principal Investigator

JIN Takashi  Osaka University, 免疫学フロンティア研究センター, 特任教授 (80206367)

Co-Investigator(Kenkyū-buntansha) 金城 政孝  北海道大学, 先端生命科学研究院, 教授 (70177971)
Co-Investigator(Renkei-kenkyūsha) KINJO Masataka  北海道大学, 大学院・先端生命科学研究院, 教授 (70177971)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywordsカリックスアレーン / 半導体量子ドット / 蛍光プローブ / 表面被覆 / 水溶化 / 表面修飾 / 蛍光 / プローブ / 半導体量子ドッド
Research Abstract

A novel method for preparing water-soluble highly fluorescent semiconductor quantum dots was developed using amphiphilic calixarenes as surface coating agents. The fluorescence efficiency of the resulting quantum dots was about 5 times higher than that of the quantum dots prepared by using simple thiol compounds as surface coating agents. The surface of the calixarene-coated quantum dots can be easily modified with biomolecules such as antibodies, showing their utility as fluorescent probes for live cell imaging.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (34 results)

All 2010 2009 2008 2007 Other

All Journal Article (22 results) (of which Peer Reviewed: 22 results) Presentation (5 results) Book (1 results) Remarks (3 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] 蛍光性半導体ナノ粒子(量子ドット)の合成、表面修飾とその医療応用2010

    • Author(s)
      神隆
    • Journal Title

      粉体工学会誌 47

      Pages: 646-655

    • NAID

      10026665510

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Visualization of microvascular blood flow in mouse kidney and spleen by quantum dot injection with “in vivo cryotecnique"2010

    • Author(s)
      Nobuo Terada, Yurika Saitoh, Sei Saitoh, Nobuhiko Ohno, Takashi Jin, Shinichi Ohno
    • Journal Title

      Microvasc Res.

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Antibody-ProteinA conjugated quantum dots for multiplexed imaging of surface receptors in living cells2010

    • Author(s)
      Takashi Jin, Dhermendra K.Tiwari Shin-ichi Tanaka, Yasushi Inouye, Keiko Yoshizawa, Tomonobu M.Watanabe
    • Journal Title

      Molecular.BioSystems 6

      Pages: 2325-2332

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Real-time Nanoscopy by using Blinking Enhanced Quantum Dots2010

    • Author(s)
      Tomonobu M.Watanabe, Shingo, Fukui, Takashi Jin, Fumihiko Fujii, Toshio Yanagida
    • Journal Title

      Biophys.J. 99

      Pages: 150-152

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A quantum dot-based ratiometric pH sensor2010

    • Author(s)
      Takashi Jin, Akira Sasaki, Masataka Kinjo, Jun Miyazaki
    • Journal Title

      Chem.Commun. 46

      Pages: 2408-2410

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Near-infrared fluorescence detection of acetylcholine in aqueous solution using a complex of rhodamine 800 and p-sulfonatocalix[8]arene2010

    • Author(s)
      Takashi Jin
    • Journal Title

      Sensors 10

      Pages: 2438-2449

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A quantum dot-based ratiometric pH sensor2010

    • Author(s)
      T.Jin, A.Sasaki, M.Kinjo, J.Miyazaki
    • Journal Title

      Chemical Communications 46

      Pages: 2408-2410

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Near-Infrared Fluorescence Detection of Acetylcholine in Aqueous Solution Using a Complex of Rhodamine 800 and p-Sulfonato-calix[8]arene2010

    • Author(s)
      Takashi Jin
    • Journal Title

      Sensors 10

      Pages: 2438-2449

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis and chracterization of anti-HER2 antibody conjugated CdSe/CdZnS quantum dots for fluorescence imaging of breast cancer cells2009

    • Author(s)
      Dheemendra K.Tiwari,Shin-Ichi Tanaka, Yasushi Inouye, Keiko Yoshizawa, Tomonobu M.Watanabe, Takashi Jin
    • Journal Title

      Sensors 9

      Pages: 9332-9354

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis and characterization of anti-HER2 antibody conjugated CdSe/CdZnS quantum dots for fluorescence imaging of breast cancer cells2009

    • Author(s)
      Tiwari K.D., Tanaka S., Inouye Y., Yoshizawa K., Watanabe M.T., Jin T.
    • Journal Title

      Sensors 9

      Pages: 9332-9345

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gd^<3+>-functionalized near-infrared quantum dots for in vivo dual modal (fluorescence/magnetic resonance) imaging2008

    • Author(s)
      Takashi Jin, Y.Yoshioka, F.Fujii, Y.Komai, J.Seki, A.Seiyama
    • Journal Title

      Chem.Commun.

      Pages: 5764-5766

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Preparation and characterization of highly fluorescent, glutathione-coated near infrared quantum dots for in vivo fluorescence imaging2008

    • Author(s)
      Takashi Jin, F.Fujii, Y.Komai, J.Seki, A.Seiyama, Y.Yoshioka
    • Journal Title

      Int.J.Mol.Sci. 9

      Pages: 2044-2061

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interfacial recognition of acetylcholine by an amphiphilic p-sulfonatocalix[8]arene derivative incorporated into dimirystoyl phosphatidylcholine vesicles.2008

    • Author(s)
      Takashi Jin, F.Fujii, Y.Ooi
    • Journal Title

      Sensors 8

      Pages: 6777-6790

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gd^<3+>-functionalized near-infrared quantum dots for in vivo dualmodal (fluorescence/magnetic resonance) imaging2008

    • Author(s)
      T. Jin, et al.
    • Journal Title

      Chem. Commun.

      Pages: 5764-5766

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation and characterization of highly fluorescent, glutathione-coated near infrared quantum dots for in vivo fluorescence imaging2008

    • Author(s)
      T. Jin, et al.
    • Journal Title

      Int. J. Mol. Sci. 9

      Pages: 2044-2061

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interfacial recognition of acetylcholine by an amphiphilic p-sulfonatocalix[8]arene derivative incorporated into dimyristoyl phosphatidylcholine vesicles2008

    • Author(s)
      T. Jin, et al.
    • Journal Title

      Sensors 8

      Pages: 6777-6790

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation and characterization of thiacalix[4]arene coated water- soluble CdSe/ZnS quantum dots as a fluorescent probe for Cu^<2+> ions2007

    • Author(s)
      Takashi Jin, F.Fujii, E.Yamada, Y.Nodasaka, M.Kinjo
    • Journal Title

      Combinatorial Chemistry & High Throughput Screening 10(6)

      Pages: 473-479

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Calixarene-based photoresponsive ion carrier for the control of Na^+ flux across a lipid bilayer membrane by visible light2007

    • Author(s)
      Takashi Jin
    • Journal Title

      Material Letters 61

      Pages: 808-808

    • NAID

      120000969111

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Preparation and Characterization of Thiacalix[4]arene Coated Water-Soluble CdSe/ZnS Quantum Dots as a Fluorescent Probe for Cu^<2+>Ions2007

    • Author(s)
      Takashi Jin
    • Journal Title

      Combinatorial Chemistry & High Throughput Screening 10

      Pages: 473-479

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In-vivo Toxicity Assessment of Intravenously Injected Anti-HER2 Antibody Conjugated CdSe Quantum dots in Wistar rat.

    • Author(s)
      Dhermendar, K.Tiawari, Takashi Jin, J Behari
    • Journal Title

      Int.J.Nanomed. in press

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fluorescent platinium (Pt_5) nanoclusters : their synthesis, purification, characterization, and application to bio-imaging

    • Author(s)
      Shin-Ichi Tanaka, Jun Miyazaki, Dhermendar, K.Tiawari, Takashi Jin, Yasushi Inouye
    • Journal Title

      Angew.Chem.Int.Ed. in press

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Safety Evaluation of Different Doses of Silver Nanoparticle Injected Intravenously in Wistar Rat

    • Author(s)
      Dhermendar, K.Tiawari, Takashi Jin, J Behari
    • Journal Title

      Toxicology Mechanism and Methods in press

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Presentation] Development of bioimaging probes based on semiconductor nanocrystals2010

    • Author(s)
      Fujii Fumihiko, Takashi Jin
    • Organizer
      IFREC-CSI Joint Symposium
    • Place of Presentation
      Hangzhou, China
    • Related Report
      2009 Final Research Report
  • [Presentation] 蛍光性半導体ナノ粒子の合成, 表面修飾とその医療応用2009

    • Author(s)
      神隆
    • Organizer
      第47回 粉体に関する討論会
    • Place of Presentation
      八王子市学園都市センター
    • Year and Date
      2009-11-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] Development of highly-fluorescent compact quantum dots from visible to near-infrared region for immune imaging2009

    • Author(s)
      Fumihiko Fujii, Takashi Jin
    • Organizer
      International Symposium organized by JST & IFReC “Frontier Immuno-Imaging"
    • Place of Presentation
      Osaka
    • Year and Date
      2009-05-11
    • Related Report
      2009 Final Research Report
  • [Presentation] Development of Highly-Fluorescent Compact Quantum Dots from Visible to Near-Infrared Region for Immune Imaging2009

    • Author(s)
      Fumihiko Fujii, Takashi Jin
    • Organizer
      Integrating Immune Networks with Immuno-Imaging Singapore Immunology Network (SIgN) & IFReC
    • Place of Presentation
      Singapore
    • Related Report
      2009 Final Research Report
  • [Presentation] Development of Highly-Fluorescent HER2-Antibody Conjugated Quantum dots for Breast Cancer Imaging2009

    • Author(s)
      Dhermendra Tiwari, Takashi Jin
    • Organizer
      "Integrating Immune Networks with Immuno-Imaging" Singapore Immunology Network (SIgN) & IFReC
    • Place of Presentation
      Singapore
    • Related Report
      2009 Final Research Report
  • [Book] 量子ドットの生命科学領域への応用2007

    • Author(s)
      神隆
    • Publisher
      シーエムシー出版
    • Related Report
      2007 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.ifrec.osaka-u.ac.jp/jpn/laboratory/nano-biomaterials/outline.php

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.ifrec.osaka-u.ac.jp/jpn/laboratory/nano-biomaterials/outline.php

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.ifrec.osaka-u.ac.jp/jpn/laboratory/nano-biomaterials/outline.php

    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] 水溶性蛍光材料およびその製造方法2009

    • Inventor(s)
      神隆, 他4名
    • Industrial Property Rights Holder
      北海道大学、科学技術振興機構
    • Acquisition Date
      2009-01-16
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 水溶性近赤外蛍光材料およびマルチモーダル水溶性近赤外蛍光材料2008

    • Inventor(s)
      神隆, 他6名
    • Industrial Property Rights Holder
      大阪大学
    • Industrial Property Number
      2008-130884
    • Filing Date
      2008-05-19
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 特許2008

    • Inventor(s)
      神隆, 他6名
    • Industrial Property Rights Holder
      大阪大学
    • Industrial Property Number
      2008-130884
    • Filing Date
      2008-05-19
    • Related Report
      2008 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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