Development of Nitric Oxide Responsible Peptide-Metal Complex Conjugate and Its Application

• Project/Area Number: 19550170
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Konan University
• Principal Investigator: FUJII Satoshi Konan University, フロンティアサイエンス学部, 准教授 (80271518)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 生物無機化学 / ジチオスルバメート錯体 / 鉄錯体 / 一酸化窒素 / センサータンパク質 / ヘリックスバンドル / ペプチド / ドテッグデリバリーシステム / 蛍光物質 / ジチオカルバメート錯体 / ドラッグデリバリーシステム

Research Abstract

We succeeded in developing an iron complex that can make a three helix bundle structure with helical peptides attached to the dithiocarbamate ligand. This iron complex has high affinity toward nitric oxide. Thermal stability of the bundle structure can be controlled by the peptide length and the bundle is stable at around 37℃ with 22 amino acid residues. Moreover, the bundle is collapsed with the nitric oxide binding to the iron ion. These results suggest that this complex can be used as a carrier for drug delivery system that responds to inflammation and nitric oxide.

Research Products

• [Journal Article] Geometric and electronic structure difference between the type 3 copper sites of the multicopper oxidases and hemocyanin/tyrosinase (2009)
  • Author(s): Jungjoo Yoon, Satoshi Fujii, Edward I.Solomon
  • Journal Title: Proc. Natl. Acad. Sci. USA 106 Pages: 6585-6590
  • Peer Reviewed
• [Journal Article] Reaction of iron(III)-polyamino carboxy-late complexes with hydrogen peroxide: Correlation between ligand structure and reactivity (2008)
  • Author(s): Satoshi Fujii, Chiyo Tsueda, Kunio Yamabe, Kohsuke Nakajima, Hiroshi Sakai
  • Journal Title: Inorg. Chim. Acta 361 Pages: 1207-1211
  • Peer Reviewed
• [Journal Article] Spectroscopic Studies of Perturbed T1 Cu Sites in the Multicopper Oxidases Saccharomyces cerevisiae Fet3p and Rhus vernicifera Laccase: Allosteric Coupling between the T1 and trinuclear Cu sites (2008)
  • Author(s): Anthony J. Augustine, Mads E. Kragh, Ritimukta Sarangi, Satoshi Fujii, Barry Liboiron, Christopher S. Stoj, Daniel J. Kosman, Keith O. Hodgson, Britt Hedman, Edward I. Solomon
  • Journal Title: Biochemistry 47 Pages: 2036-2045
  • Peer Reviewed
• [Presentation] Triple Helix Bundle構造をとるペプチドージチオカルバメート鉄錯体においてペプチド長がBundle構造安定性に及ぼす影響 (2008)
  • Author(s): 山中敏志, 西村宗十、藤井敏司、酒井宏、杉本直己
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 横浜(東京工業大学すずかけ台キャンパス)
  • Year and Date: 2008-09-18
• [Presentation] Triple Helix Bundle構造をとるペプチドージチオカルバメー卜鉄錯体錯体においてペプチド長がBundle構造安定性に及ぼす効果 (2008)
  • Author(s): 山中, 西村, 藤井, 酒井, 杉本
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 東京工業大学長津田キャンパス
  • Year and Date: 2008-09-18
• [Presentation] チトクロムc'から内因性NOのESR画像化まで (2008)
  • Author(s): 藤井敏司
  • Organizer: 第8回日本NO学会学術集会
  • Place of Presentation: 仙台(仙台国際センター)
  • Year and Date: 2008-05-09
• [Presentation] NanoBioNow(13)一酸化窒素感受性を有するThree Helix Bundle型ペプチドー鉄ジチオカルバメート錯体の合成と性質 (2008)
  • Author(s): 矢口真弓、西村宗十、藤井敏司、酒井宏、杉本直己
  • Organizer: 日本化学会第88回春季年会
  • Place of Presentation: 東京(立教大学)
  • Year and Date: 2008-03-30
• [Presentation] NanoBio Now(13):一酸化窒素感受性を有するThree Helix Bundle型ペプチドー鉄ジチオカルバメート錯体の合成と性質 (2008)
  • Author(s): 藤井 敏司
  • Organizer: 日本化学会第88回春季年会
  • Place of Presentation: 立教大学(東京都豊島区)
  • Year and Date: 2008-03-29
• [Presentation] Biological applications of EPR: from in vivo NO detection/imaging to Cu(II) coordination toβ-amyloid (2007)
  • Author(s): Satoshi Fujii
  • Organizer: Molecular Photoscience Research Center International Workshop: Low Energy Excitation in Condensed Phases
  • Place of Presentation: 神戸(神戸大学)
  • Year and Date: 2007-11-12
• [Book] 元素111の新知識第2版 (2009)
  • Author(s): 櫻井弘編、藤井敏司, 他
  • Total Pages: 461
  • Publisher: 講談社ブルーバックス
• [Book] 元素111の新知識第2版 (2009)
  • Author(s): 樫井弘, 藤井敏司, ら
  • Total Pages: 461
  • Publisher: 講談社ブルーバックス
• [Remarks] 報道関連情報日刊工業新聞、2008年5月11日記事
  • URL: http://www.konan-first.jp/graduate/ti_303_b.html