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Studies on the structure and function of hemeproteins that use advantage of introduction of fluoroalkyl groups

Research Project

Project/Area Number 19550172
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemistry related to living body
Research InstitutionNagaoka National College of Technology

Principal Investigator

SUZUKI Akihiro  Nagaoka National College of Technology, 物質工学科, 教授 (60179190)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords生物有機化学 / 合成化学 / フッ素 / ポルフィリン / ヘムタンパク質 / F-NMR
Research Abstract

Functional regulation of hemeprotein is thought to be achieved through electronic structure of heme, and heme environment furnished by nearby amino acid residues. In this study, I have performed substitution of electron-withdrawing fluoro (F) and perfluoromethyl (CF_3) group(s) as pyrrole, porphyrin and heme side chain(s) to obtain large alteration of the heme electronic structure in order to elucidate the relationship between the structure and function. I have measured the ^<19>F-NMR, and the equilibrium constant (pKa) of the "acid-alkaline transition" in metmyoglobin in order to quantitatively assess the effects of the F and CF_3 substitutions for the electron density of heme Fe atom of the hemeprotein. I have confirmed that the introduction of F or CF_3 group(s) to the heme side chain(s) leads to a decrease in the electron density of the heme Fe atom, which in turn decreases the O_2 affinity of hemeprotein.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (19 results)

All 2010 2009 2008 2007 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (15 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Effect of Heme Modification on Oxygen Affinity of Myoglobin and Equilibrium of the Acid-Alkaline Transition in Metmyoglobin2010

    • Author(s)
      T. Shibata, S. Nagao, M. Fukaya, H. Tai, S. Nagatomo, K. Morihashi, T. Matsuo, S. Hirota, A. Suzuki, K. Imai, Y. Yamamoto
    • Journal Title

      Journal of the American Chemical Society 132

      Pages: 6091-6098

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of Heme Modification on Oxygen Affinity of Myoglobin and Equilibrium of the Acid-Alkaline Transition in Metmyoglobin2010

    • Author(s)
      Tomokazu Shibata
    • Journal Title

      Journal of the American Chemical Society 132

      Pages: 6091-6098

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] ミオグロビンにおけるヘム側鎖の電子的性質とヘム鉄の電子密度及び反応性との関係2010

    • Author(s)
      深谷昌史、柴田友和、太虎林、長友重紀、山本泰彦、鈴木秋弘
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      近畿大学
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] フッ素置換基導入によるポルフィリン環の化学修飾2010

    • Author(s)
      高橋香織、坂井優介、鈴木秋弘
    • Organizer
      第15回高専シンポジウムinいわき
    • Place of Presentation
      いわき市文化センター
    • Year and Date
      2010-01-23
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] Analysis of Subunit「Interaction in Human Adult and Fetal Hemoglobins through the characterization of the Acid-Alkaline Transition」2009

    • Author(s)
      T. Shibata, S. Nagao, H. Tai, S. Nagatomo, H. Hamada, H. Yoshikawa, A. Suzuki, Y. Yamamoto
    • Organizer
      14th International Conference on Biological Inorganic Chemistry
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2009-07-27
    • Related Report
      2009 Final Research Report
  • [Presentation] Analysis of Subunit Interaction in Human Adult and Fetal Hemoglobins through the characterization of the Acid-Alkaline Transition2009

    • Author(s)
      Tomokazu Shibata
    • Organizer
      14th International Conference on Biological Inorganic Chemistry
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2009-07-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] Relationship between Oxygen Affinity of Myoglobin and the Equilibrium constant of Acid-Alkaline Transition in Metmyoglobin2009

    • Author(s)
      Tomokazu Shibata
    • Organizer
      14th International Conference on Biological Inorganic Chemistry
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2009-07-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] フッ素化ヘム再構成ヘモグロビン(Hb)の19F NMRによる機能解析2008

    • Author(s)
      水関和哉、太虎林、長友重紀、山本泰彦、鈴木秋弘
    • Organizer
      第47回NMR討論会P040
    • Place of Presentation
      筑波大学
    • Year and Date
      2008-11-14
    • Related Report
      2009 Final Research Report
  • [Presentation] フッ素化ヘム再構成ヘモグロビン(Hb)の^<19>F NMR による機能解析2008

    • Author(s)
      山本泰彦
    • Organizer
      第47回NMR討論会
    • Place of Presentation
      筑波大学
    • Year and Date
      2008-11-14
    • Related Report
      2008 Annual Research Report
  • [Presentation] フッ素化ヘム再構成ヘムタンパク質の19F NMRシグナルの磁場依存性の解析2008

    • Author(s)
      宮崎泰斗、太虎林、長友重紀、山本泰彦、鈴木秋弘
    • Organizer
      第47回NMR討論会P041
    • Place of Presentation
      筑波大学
    • Year and Date
      2008-11-12
    • Related Report
      2009 Final Research Report
  • [Presentation] フッ素化ヘム再構成ヘムタンパク質の^<19>F NMR シグナルの磁場依存性の解析2008

    • Author(s)
      山本泰彦
    • Organizer
      第47回NMR討論会
    • Place of Presentation
      筑波大学
    • Year and Date
      2008-11-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] C_2対称フッ素化ヘム再構成ヘムタンパク質の活性部位構造の^<19>F NMRによる解析2008

    • Author(s)
      山本泰彦
    • Organizer
      第47回NMR討論会
    • Place of Presentation
      筑波大学
    • Year and Date
      2008-11-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] C2対称フッ素化ヘム再構成ヘムタンパク質におけるFe-His配位結合の19F NMRによる解析2008

    • Author(s)
      水関和哉、宮崎泰斗、柴田友和、古市英資、長友重紀、河野慎、山本泰彦、鈴木秋弘
    • Organizer
      日本化学会第88春季年会
    • Place of Presentation
      立教大学池袋キャンパス
    • Year and Date
      2008-03-27
    • Related Report
      2009 Final Research Report
  • [Presentation] ヘム側鎖にフッ素をもつヘムタンパク質の19F NMR2007

    • Author(s)
      山本泰彦、長尾聡、水関和哉、河野慎、長友重紀、鈴木秋弘
    • Organizer
      第46回NMR討論会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2007-09-12
    • Related Report
      2009 Final Research Report
  • [Presentation] ヘム側鎖にフッ素をもつヘムタンパク質の19F NMR2007

    • Author(s)
      山本泰彦
    • Organizer
      第46回NMR討論会
    • Place of Presentation
      第46回NMR討論会
    • Year and Date
      2007-09-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] フッ素化ヘム再構成酸化型ヘモグロビンにおける酸塩基平衡の19F NMR研究2007

    • Author(s)
      長友重紀、長尾聡、水関和哉、河野慎、山本泰彦、鈴木秋弘、今井清博
    • Organizer
      第34回生体分子科学討論会
    • Place of Presentation
      東北大学
    • Year and Date
      2007-06-22
    • Related Report
      2009 Final Research Report 2007 Annual Research Report
  • [Presentation] NMR測定用フッ素核プローブの開発とその応用2007

    • Author(s)
      鈴木秋弘
    • Organizer
      第6回産官学連携推進会議
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2009 Final Research Report
  • [Book] 物質工学入門シリーズ「基礎からわかる機器分析」2010

    • Author(s)
      加藤正直, 内山一美, 鈴木秋弘
    • Publisher
      森北出版
    • Related Report
      2009 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.nagaoka-ct.ac.jp/mb/index.files/Page1144.htm

    • Related Report
      2009 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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