Factors causing sudden increase in the transposition frequency of transposable elements in medaka fish

• Project/Area Number: 19570003
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: Kyoto University (2008); Nagoya University (2007)
• Principal Investigator: KOGA Akihiko Kyoto University, 霊長類研究所, 教授 (80192574)
• Co-Investigator(Kenkyū-buntansha): SHIMADA Atsuko 東京大学, 大学院・理学系研究科, 助教 (20376552)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 転移因子 / トランスポゾン / 転移頻度 / 突然変異 / 遺伝的変異 / メダカ / 転移酵素遺伝子 / ゲノム

Research Abstract

メダカのTol1因子では、転移頻度がある世代で突然上昇するという現象が起こる。この現象の分子レベルでの機構を探るために、転移酵素遺伝子をもつ因子のメダカへの導入と、転移頻度の測定を主体とする実験を、行った。高い転移頻度を示したメダカでは、因子の挿入は、遺伝子の下流で起こっていた。この結果から、上流の遺伝子からのリードスルーが転移酵素mRNAの増加をもたらし、これが転移頻度の上昇につながると、推測した。

Research Products

• [Journal Article] The Tol1 element of the medaka fish, a member of the hAT transposable element family, jumps in Caenorhabditis elegans (2008)
  • Author(s): Kodama K, Takagi S, Koga A
  • Journal Title: Heredity 101 Pages: 222-227
  • Peer Reviewed
• [Journal Article] Germline transgenesis of zebrafish using the medaka Tol1 transposon system (2008)
  • Author(s): Koga A, Cheah FS, Hamaguchi S, Yeo GH Chong SS
  • Journal Title: Developmental Dynamics 237 Pages: 2466-2474
  • Peer Reviewed
• [Journal Article] Germline transgenesis of zebrafish using the medaka To11 transposon system (2008)
  • Author(s): Koga A, Cheah FS, Hamaguchi S, Yeo GH Chong SS
  • Journal Title: Developmental Dynamics 237 Pages: 2466-2474
  • Peer Reviewed
• [Journal Article] The Tol1 element of the medaka fish, a member of the hAT transposable element family, jumps in Caenorhabditis elegans (2008)
  • Author(s): Kodama K. Takagi S, Koga A
  • Journal Title: Heredity 101 Pages: 222-227
  • Peer Reviewed
• [Journal Article] The Tol1 transposable element of the medaka fish moves in human and mouse cells (2007)
  • Author(s): Koga A*, Shimada A, Kuroki T, Hori H, Kusumi J, Kyono-Hamaguchi Y, Hamaguchi S
  • Journal Title: Journal of Human Genetics 52 Pages: 628-635
  • Peer Reviewed
• [Journal Article] The Tol1 element of medaka fish is transposed with only terminal regions and can deliver large DNA fragments into the chromosomes (2007)
  • Author(s): Koga A*, Higashide I, Hori H, Wakamatsu Y, Kyono Hamaguchi Y, Hamaguchi S
  • Journal Title: Journal of Human Genetics 52 Pages: 1026-1030
  • NAID: 10020022369
  • Peer Reviewed
• [Journal Article] PCR detection of excision suggests mobility of the medaka fish Tol1. transposable element in the frog Xenopus laevis (2007)
  • Author(s): Hikosaka A, Koga A*
  • Journal Title: Genetical Research 89 Pages: 201-206
  • Peer Reviewed
• [Journal Article] The Toll transposable element of the medaka fish moves in human and mouse cells. (2007)
  • Author(s): Koga A, Shimada A, Kuroki T, Hori H, Kusumi J, Kyono-Hamaguchi Y, Hamaguchi S
  • Journal Title: Journal of Human Genetics 52 Pages: 628-635
  • NAID: 40015454172
  • Peer Reviewed
• [Journal Article] The Toll element of medaka fish is transposed with only terminal regions and can deliver large DNA fragments into the chromosomes. (2007)
  • Author(s): Koga A, Higashide I, Hori H, Wakamatsu Y, Kyono-Hamaguchi Y, Hamaguchi S
  • Journal Title: Journal of Human Genetics 52 Pages: 1026-1030
  • Peer Reviewed
• [Journal Article] PCR detection of excision suggests mobility of the medaka fish Toll transposable element in the frog Xenopus laevis. (2007)
  • Author(s): Hikosaka A, Koga A
  • Journal Title: Genetical Research 89 Pages: 201-206
  • Peer Reviewed
• [Presentation] Tol1 and Tol2 of medaka : two similar hAT-family elements at different evolutionary stages in a single fish genome (2009)
  • Author(s): Koga A
  • Organizer: 2nd International Conference and Workshop : Genomic Impact of Eukaryotic Transposable Elements
  • Place of Presentation: Pacific Grove, CA, USA
  • Year and Date: 2009-02-09
• [Presentation] メダカのトランスポゾン Tol1のCaenorhabditis elegansでの転移 (2008)
  • Author(s): 児玉健・古賀章彦・高木新
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-10
• [Presentation] メダカのトランスポゾンTo11のCaenorhabditis elegans での転移 (2008)
  • Author(s): 児玉健, 古賀章彦, 高木新
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-10
• [Presentation] Tol1 and Tol2 of medaka : two similar hAT-family elements at different evolutionary stages in a single fish genome (2008)
  • Author(s): Koga A.
  • Organizer: 2nd International Conference and Workshop "Genomic Impact of Eukaryotic Transposable Elements"
  • Place of Presentation: Pacific Grove, CA, USA
  • Year and Date: 2008-12-10
• [Presentation] メダカのトランスポゾンTol1 : 転移触媒酵素の構造・機能・変異 (2008)
  • Author(s): 古賀章彦、濱口哲
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2008-12-09
• [Presentation] hATファミリーのトランスポゾンであるメダカのTol1因子は旧口動物と新口動物の両方で転移する (2008)
  • Author(s): 古賀章彦
  • Organizer: 日本遺伝学会第80回大会
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-09-05
• [Presentation] 脊椎動物のトランスポゾン : Tol1因子の転移酵素遺伝子はメダカ自然集団で完全な消滅に至っていない (2008)
  • Author(s): 古賀章彦・濱口哲・酒泉満
  • Organizer: 第10回日本進化学会大会
  • Place of Presentation: 東京都
  • Year and Date: 2008-08-22
• [Presentation] 脊椎動物のトランスポゾン : To11因子の転移酵素遺伝子はメダカ自然集団で完全な消滅に至っていない (2008)
  • Author(s): 古賀章彦, 濱口哲, 酒泉満
  • Organizer: 第10回日本進化学会大会
  • Place of Presentation: 東京都
  • Year and Date: 2008-08-22
• [Presentation] メダカのトランスポゾンToll:転移酵素遺伝子の構造・機能・変異 (2007)
  • Author(s): 古賀 章彦
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2007-12-11
• [Patent(Industrial Property Rights)] Tol1因子のトランスポザーゼ及びそれを用いたDNA導入システム (2007)
  • Inventor(s): 古賀章彦・濱口哲
  • Industrial Property Rights Holder: 名古屋大学・新潟大学
  • Filing Date: 2007-12-06
  • Overseas