Development of genetic system of rRNA genes and its use in B. subtilis
| Project/Area Number |
19570006
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Rikkyo University |
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Principal Investigator |
KAWAMURA Fujio Rikkyo University, 理学部, 教授 (10126039)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | リボソーム / rRNA / 翻訳 / 枯草菌 / 胞子形成 / rRNAオペロン / 抗生物質耐性 |
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| Research Abstract |
We deleted the 10 rrn operons of B. subtilis sequentially, and constructed seven mutants, each harboring a single rrn operon in their genome. The growth rates and sporulation frequencies of these mutants were reduced drastically as compared with those of the wild type strain, indicating that normal growth as well as sporulation requires multiple copy numbers of rRNA operon. Further analysis using mutants having various numbers of rRNA operon showed that normal sporulation requies more than 5 copies of rRNA operon.
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Report
(4 results)
Research Products
(59 results)
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[Journal Article] Inhibition of Septation in Bacillus subtilis by a peptide antibiotic2010
Author(s)
Shimotohno, W.K., Kawamura F., Natori, H., Nanamiya, H., Magae, J., Ogata, H., Endo, T., Suzuki, T., Yamaki, H.
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Journal Title
edein B1. Biol. Pharm. Bull. 33
Pages: 568-571
Related Report
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[Journal Article] Transcription activity of individual rrn operons in Bacillus subtilis mutants deficient in (p)ppGpp synthetase genes, relA, yjbM, and ywaC.2009
Author(s)
Natori, Y., Tagami, K., Murakami, K., Yoshida, S., Tanigawa, O., Moh, Y., Masuda, K., Wada, T., Suzuki, S., Nanamiya, H., Tozawa, Y., Kawamura, F.
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Journal Title
J. Bacteriol. 191
Pages: 4555-4561
Related Report
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[Journal Article] Inactivation of KsgA, a 16S rRNA methyltransferase, causes vigorous emergence of mutants with high-level kasugamycin resistance.2009
Author(s)
Ochi, K., Kim, J-Y., Tanaka, Y., Wang, G., Masuda, K., Nanamiya, H., Okamoto, S., Tokuyama, S., Adachi, Y., Kawamura, F.
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Journal Title
Antimicrob. Agents Chemother. 53
Pages: 193-201
Related Report
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[Journal Article] A fail-safe system for the ribosome under zinc-limiting conditions in Bacillus subtilis.2007
Author(s)
Natori, Y., Nanamiya, H., Akanuma, G., Kosono, S., Kudo, T., Ochi, K., Kawamura, F.
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Journal Title
Mol. Microbiol. 63
Pages: 294-307
Related Report
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[Journal Article] Isolation and characterization of sporulation-initiation mutation in the Bacillus subtilis prfB gene. Biosci.2007
Author(s)
Asai, K., Inaoka, T., Nanamiya, H., Sadaie, Y., Ochi, K., Kawamura, F.
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Journal Title
Biotechnol. Biochem. 71
Pages: 397-406
Related Report
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