Chromatin regulation around M26/CRE DNA sequences

• Project/Area Number: 19570008
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: The University of Tokyo
• Principal Investigator: OHTA Kunihiro The University of Tokyo, 大学院・総合文化研究科, 教授 (90211789)
• Co-Investigator(Kenkyū-buntansha): HIROTA Kouji 独立行政法人理化学研究所, 基礎科学特別研究員 (00342840)
• Co-Investigator(Renkei-kenkyūsha): HIROTA Kouji 京都大学, 大学院・医学系研究科, 助教 (00342840) ; YAMADA Takatomi 東京大学, 大学院・総合文化研究科, 助教 (30451850)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: クロマチン / ヒストン修飾 / 後生遺伝学 / ストレス応答 / ノンコーディングRNA / エピゲノム / ヒストン / 組換え / 転写 / 減数分裂 / 転写制御 / 染色体 / non-coding RNA / エピジェネティクス

Research Abstract

分裂酵母M26/CRE 配列の周辺におけるヒストン修飾やクロマチン再編成に関わる因子を数種同定し、それらの欠失変異株について、種々のストレスに対する感受性やヒストン修飾における役割を解明した。さらに、グルコース飢餓時に転写が活性化する分裂酵母fbp1+遺伝子座におけるクロマチン再編成に、mRNA 型の長鎖ノンコーディングRNA のカスケード転写が関わることを見出し、ノンコーディングRNA を介した新しい転写活性化モデルを提出した。

Research Products

• [Journal Article] Accumulation of sumoylated Rad52 incheckpoint mutants perturbed in DNAreplication. Ohuchi T (2009)
  • Author(s): Ohuchi T., Seki M., Kugou K., Tada S., Ohta K., and Enomoto T.
  • Journal Title: DNA repair (in press)
  • Peer Reviewed
• [Journal Article] Cascadetranscription of mRNA-type long non-codingRNAs (mlonRNAs) and local chromatinremodeling (2009)
  • Author(s): Hirota K and Ohta K
  • Journal Title: Epigenetics (in press)
  • Peer Reviewed
• [Journal Article] Ctp1^<CtIP> and the Rad32^<Mre11> nuclease activityare required for Rec12^<Spo11> removal, but thisactivity is dispensable for otherMRN-dependent meiotic functions (2009)
  • Author(s): Hartsuiker E., Mizuno K., Molna M., Kohli J., Ohta K. and Carr A.M.
  • Journal Title: Mol. Cell Biol (in press)
  • Peer Reviewed
• [Journal Article] Cascade transcription of mRNA-type long non-coding RNAs(mlonRNAs) and Iocal chromatin remodeling (2009)
  • Author(s): Hirota K. et al.
  • Journal Title: Epigenetics (印刷中掲載確定)
  • Peer Reviewed
• [Journal Article] CtplCtIP and the Rad32Mrell nuclease activity are required for Rec12Spoll removal, but this actiVity is dispensable for other MRN-dependent meiotic fiunctions. (2009)
  • Author(s): Hartsuiker E. et al.
  • Journal Title: Mol. Cell Biol. (印刷中掲載確定)
  • Peer Reviewed
• [Journal Article] Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replication (2009)
  • Author(s): Ohuchi T. et al.
  • Journal Title: DNA repair (印刷中掲載確定)
  • Peer Reviewed
• [Journal Article] Molecular characterization of the Schizosaccharomyces pombe nip1+/ctp1+gene in DNA double strand break repair in association with the Mre11-Rad50-Nbs1 complex. (2009)
  • Author(s): Akamatsu Y. et al.
  • Journal Title: Mol. Cell Biol. 28 Pages: 3639-3651
  • Peer Reviewed
• [Journal Article] Essential roles of Snf21, aSwi2/Snf2 family chromatin remodeler, infission yeast mitosis (2008)
  • Author(s): Yamada K., Hirota K., Mizuno K, Shibata T., and Ohta K.
  • Journal Title: Genes & Genet 83 Pages: 361-372
  • Peer Reviewed
• [Journal Article] In-cell viscosity measurement using afluorescence up-conversion microscope (2008)
  • Author(s): Fujino T., Hirota K., Ohta K. and Tahara T.
  • Journal Title: Chemistry Letters 37 Pages: 1240-1241
  • Peer Reviewed
• [Journal Article] Stepwise chromatin remodeling by a cascadeof transcription initiation of non-coding RNAs (2008)
  • Author(s): Hirota K., Miyoshi T., Kugou K., Hoffman C.S., Shibata T., and Ohta K.
  • Journal Title: Nature 456 Pages: 130-134
  • Peer Reviewed
• [Journal Article] Structural and functional analyses of theDMC1-M200V polymorphism found in thehuman population.Nucl (2008)
  • Author(s): Hikiba J., Hirota K., Kagawa W., Ikawa S., Kinebuchi T., Sakane I., Takizawa, Yokoyama S., Mandon-Pepin B., Nicolas A., Shibata S., Ohta K., and Kurumizaka H
  • Journal Title: Acids Res 36 Pages: 4181-4190
  • Peer Reviewed
• [Journal Article] Ino80chromatin remodeling complex promotesrecovery of stalled replication forks (2008)
  • Author(s): Shimada K., Oma Y., Schleker T., Kugou K., Ohta K., Harata M. and Gasser SM.
  • Journal Title: Curr. Biol 18 Pages: 566-575
  • Peer Reviewed
• [Journal Article] Molecular characterization of theSchizosaccharomyces pombe nip1+/ctp1+ genein DNA double strand break repair inassociation with the Mre11-Rad50-Nbs1complex (2008)
  • Author(s): Akamatsu Y., Murayama Y., Yamada T., Nakazaki T., Tsutsui Y., Ohta K., and Iwasaki H.
  • Journal Title: Mol. Cell Biol 28 Pages: 3639-3651
  • Peer Reviewed
• [Journal Article] Distinct chromatin modulatorsregulate to form accessible and repressivechromatin in fission yeast recombinationhotspot ade6-M26 (2008)
  • Author(s): Hirota K., Mizuno K., Shibata T., and Ohta K.
  • Journal Title: Mol.Biol.Cell 19 Pages: 1162-1173
  • Peer Reviewed
• [Journal Article] Regulation of histone H4acetylation by transcription factor E2A inimmunoglobulin gene conversion (2008)
  • Author(s): Kitao H., Kimura M., Yamamoto K., Seo H., Namikoshi K., Agata Y., Ohta K., and Takata M.
  • Journal Title: Int. Immunol 20 Pages: 277-284
  • Peer Reviewed
• [Journal Article] Modulation ofimmunoglobulin gene conversion frequencyand distribution by the histone deacetylaseHDAC2 in chicken DT40 (2008)
  • Author(s): Lin W., Hashimoto S., Seo H., Shibata T., and Ohta K
  • Journal Title: Genes to Cells 3 Pages: 255-268
  • Peer Reviewed
• [Journal Article] Cdc7-dependent phosphorylation of Mer2facilitates initiation of yeast meioticrecombination (2008)
  • Author(s): Sasanuma H., Hirota K., Fukuda T., Kakusho N., Kugou K., Kawasaki Y., Shibata T., Masai H., and Ohta K.
  • Journal Title: Genes Dev 22 Pages: 398-410
  • Peer Reviewed
• [Journal Article] Targeted induction of meiotic double-strandbreaks reveals chromosomaldomain-dependent regulation of Spo11 andinteractions among potential sites of meioticrecombination.Nucl (2008)
  • Author(s): Fukuda T., Kugou K., Sasanuma H., Shibata T., and Ohta K.
  • Journal Title: Acids Res 36 Pages: 984-997
  • Peer Reviewed
• [Journal Article] Essential roles of Snf21, a Swi2/Snf2 family chromatin remodeler, in fission yeast mitosis. (2008)
  • Author(s): Yamada K. et al.
  • Journal Title: Genes & Genet. Sys. 83 Pages: 361-372
  • Peer Reviewed
• [Journal Article] Stepwise chromatin remodeling by a cascade of transcription initiation of non-codin RNAs. (2008)
  • Author(s): Hirota K. et al.
  • Journal Title: Nature 456 Pages: 130-134
  • Peer Reviewed
• [Journal Article] Structural and fUnctional analyses of the DMC1-M200V polymorphism found in the human population. (2008)
  • Author(s): Hikiba J. et al.
  • Journal Title: Nucl. Acids Res. 36 Pages: 4181-4190
  • Peer Reviewed
• [Journal Article] Ino80 chromatin remodeling complex promotes recovery of stalled replication forks. (2008)
  • Author(s): Shimda K. et al.
  • Journal Title: Curr. Biol. 18 Pages: 566-575
  • Peer Reviewed
• [Journal Article] In-cell Viscosity measurement using a fluorescence up-conversion microscope. (2008)
  • Author(s): Fujino T. et al.
  • Journal Title: Chemistry Lett. 37 Pages: 1240-1241
  • Peer Reviewed
• [Journal Article] Cdc7-dependent phosphorylation of Mer2 facilitates initiation of yeas tmeiotic recombination (2008)
  • Author(s): Sasanuma H., et. al.
  • Journal Title: Genes & Development 22 Pages: 398-410
  • Peer Reviewed
• [Journal Article] Targeted induction of meiotic double-strand breaks reveals chromosomal domain-dependent regulation of Spo11 and interactions among potential sites of meiotic recombination (2008)
  • Author(s): Fukuda T., et. al.
  • Journal Title: Nucleic Acids Research 36 Pages: 984-997
  • Peer Reviewed
• [Journal Article] Multiple modes of chromatinconfiguration at natural meiotic recombinationhotspots in fission yeas (2007)
  • Author(s): Hirota K., Steiner W.W., Shibata T., and Ohta K.
  • Journal Title: Euk. Cell 6 Pages: 2072-2080
  • Peer Reviewed
• [Journal Article] Ohta K.Conditional genomic rearrangement bydesigned meiotic recombination using VDE(PI-SceI) in yeast.Mol (2007)
  • Author(s): Fukuda T., Ohya Y., and Ohta K.
  • Journal Title: Genet. Genomics 278 Pages: 467-478
  • Peer Reviewed
• [Journal Article] Actin-relatedprotein Arp4 functions in kinetochoreassembly (2007)
  • Author(s): Ogiwara H., Ui, A., Kawashima S.,Kugou K., Onoda F., Iwahashi H., Harata M.,Ohta K., Enomoto T., Seki M
  • Journal Title: Nucl. Acids Res 35 Pages: 3109-3117
  • Peer Reviewed
• [Journal Article] Mre11 mediatesgene regulation in yeast spore development (2007)
  • Author(s): Kugou K., Sasanuma H., Matsumoto K., Shirahige K., and Ohta K.
  • Journal Title: Genes & Genet. Sys 82 Pages: 21-33
  • Peer Reviewed
• [Journal Article] Meioticassociation between Spo11 regulated byRec102, Rec104, and Rec114.Nucl (2007)
  • Author(s): Sasanuma H., Murakami H., Fukuda T., Shibata T., Nicolas A., and Ohta K
  • Journal Title: Acids Res 35 Pages: 1119-1133
  • Peer Reviewed
• [Journal Article] Regulation of histone H4 acetylation by transcription factor E2A in immunoglobulin gene conversion (2007)
  • Author(s): Kitao H., et. al.
  • Journal Title: Int. Immunol. 20 Pages: 277-284
  • Peer Reviewed
• [Journal Article] Modulation of immunoglobulin gene conversion frequency and distribution by the histone deacetylase HDAC2 in chicken DT40 (2007)
  • Author(s): Lin W., et. al.
  • Journal Title: Genes to Cells 3 Pages: 255-268
  • Peer Reviewed
• [Journal Article] Multiple modes of chromatin configuration at natural meiotic recombination hotspots in fission yeast (2007)
  • Author(s): Hirota K., et. al.
  • Journal Title: Eukaryotic Cell 6 Pages: 2072-2080
  • Peer Reviewed
• [Journal Article] Conditional genomic rearrangement by designed meiotic recombination using VDE (Pl-Scel) in yeast. (2007)
  • Author(s): Fukuda T., et. al.
  • Journal Title: Mol. Genet. Genomics 278 Pages: 467-478
  • Peer Reviewed
• [Journal Article] Actin-related protein Arp4 functions in kinetochore assembly (2007)
  • Author(s): Ogiwara H., et. al.
  • Journal Title: Nucleic Acids Research 35 Pages: 3109-3117
  • Peer Reviewed
• [Presentation] Chromatin control of CRE-regulated recombination and geneactivation (2008)
  • Author(s): 太田邦史ら
  • Organizer: 米国細胞生物学会
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2008-12-17
• [Presentation] Chromatin control of CRE-regulated recombination and gene activation (2008)
  • Author(s): Ohta et al.
  • Organizer: American Society of Cell Biology Annual Meeting 2008
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2008-12-17
• [Presentation] Chromatin regulation of stress-induced gene activation (2008)
  • Author(s): 太田邦史ら
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] Chromatin regulation ofstress-induced gene activation (2008)
  • Author(s): 太田邦史ら
  • Organizer: 日本分子生物学会ワークショップ
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] ATF/CREB ファミリータンパク質による減数分裂諸現象の総合的制御 (2008)
  • Author(s): 山田貴富ら
  • Organizer: 日本分子生物学会ワークショップ
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] ATF/CREBファミリータンパク質による減数分裂諸現象の総合的制御 (2008)
  • Author(s): 山田貴富ら
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] Cell-cycle control of meioticrecombination initiation (2008)
  • Author(s): 太田邦史ら
  • Organizer: 6^<th> 3R meeting
  • Place of Presentation: 嬬恋・静岡
  • Year and Date: 2008-10-28
• [Presentation] Cell-cycle control of meiotic recombination initiation (2008)
  • Author(s): Ohta et al.
  • Organizer: 6th 3R meeting
  • Place of Presentation: 嬬恋、静岡
  • Year and Date: 2008-10-28
• [Presentation] 分裂酵母ATF/CREBファミリータンパク質Atf21の機能解析 (2008)
  • Author(s): 山田貴富ら
  • Organizer: 日本遺伝学会
  • Place of Presentation: 札幌
  • Year and Date: 2008-09-11
• [Presentation] 分裂酵母クロマチン再編成因子Snf21の機能解析 (2008)
  • Author(s): 山田健太郎ら
  • Organizer: 日本遺伝学会
  • Place of Presentation: 札幌
  • Year and Date: 2008-09-11
• [Presentation] 減数分裂組換え開始のDNAトランスアクション (2008)
  • Author(s): 太田邦史ら
  • Organizer: 日本遺伝学会シンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 2008-09-05
• [Presentation] 減数分裂組換え開始のDNAトランスアクション (2008)
  • Author(s): 太田邦史ら
  • Organizer: 日本遺伝学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-09-05
• [Presentation] Control of meioticrecombination initiation by Cdc7-Dbf4 (2008)
  • Author(s): 太田邦史ら
  • Organizer: Gordon conference on "Meiosis"
  • Place of Presentation: Colby-Sawyer College, NH, USA
  • Year and Date: 2008-06-11
• [Presentation] Contro lof meiotic recombination initiation by Cdc7-Dbf4 (2008)
  • Author(s): Ohta et al.
  • Organizer: Gordon conference on"Meiosis"
  • Place of Presentation: Colby-Sawyer College, USA
  • Year and Date: 2008-06-11
• [Presentation] 鳥類抗体偽可変領域遺伝子クラスターにおける遺伝子変換による抗体遺伝子創出機能 (2007)
  • Author(s): 太田邦史
  • Organizer: 日本分子生物学会(ワークショップ)
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
• [Presentation] 減数分裂期のおけるCdc7キナーゼの役割 (2007)
  • Author(s): 笹沼博之
  • Organizer: 日本分子生物学会(ワークショップ)
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-13
• [Presentation] 減数分裂期相同組換え開始酵素Spo11の活性化機構 (2007)
  • Author(s): 太田邦史ら
  • Organizer: 日本遺伝学会シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 2007-09-19
• [Presentation] 減数分裂期相同組換え開始酵素Spol1の活性化機構 (2007)
  • Author(s): 太田邦史
  • Organizer: 日本遺伝学会(シンポジウム)
  • Place of Presentation: 岡山大学
  • Year and Date: 2007-09-19
• [Presentation] Dynamic distribution ofmeiotic chromosomal proteins along yeastchromosomes (2007)
  • Author(s): 太田邦史ら
  • Organizer: EMBO workshop onMeiosis
  • Place of Presentation: 湘南国際村、神奈川
  • Year and Date: 2007-09-15
• [Book] In Methods in Molecular Biology"Meiosis : Vol 1, Molecular and GeneticMethod"(in press) (2009)
  • Author(s): Hirota K., Fukuda T., Yamada T., and Ohta K.
  • Publisher: Analysis of chromatin structure at meiotic DSB stes in yeasts
• [Book] In Methods in Molecular Biology"Meiosis : Vol 1, Molecular and GeneticMethod"(eds Scott Keeney, Springer)(in press) (2009)
  • Author(s): Kugou K., and Ohta K.
  • Publisher: Genome-wide high-resolution chromatin immunoprecipitation of meiotic chromosomal proteins in S. cerevisiae
• [Book] Communicative & Integrative Biology(in press) (2009)
  • Author(s): Hirota K., and Ohta K.
  • Publisher: Transcription of mRNA-type long non-coding RNAs (mlonRNAs) disrupts chromatin array
• [Book] 化学と生物 (2009)
  • Author(s): 廣田耕志、太田邦史
  • Publisher: mlonRNA 仮説 : ノンコーディングRNA 転写による新規クロマチン構造変化機構
• [Book] 蛋白質核酸酵素 (2009)
  • Author(s): 廣田耕志、太田邦史
  • Publisher: mlonRNA : クロマチン再編成を通じて遺伝子活性化にかかわるノンコーディングRNA
• [Book] 分子細胞治療 (2009)
  • Author(s): 太田邦史、瀬尾秀宗、橋本修一
  • Publisher: ADLib[○!R]システム
• [Book] 蛋白質核酸酵素増刊 (2009)
  • Author(s): 笹沼博之、太田邦史
  • Publisher: 減数分裂期特異的DNA 二重鎖切断機構 in 染色体サイクル
• [Book] Modulation of immunoglobulin gene conversion in chicken DT40 by enhancing histone acetylation and its application to antibody engineering (2007)
  • Author(s): Seo H., Yamada T., Hashimoto S., Lin W., and Ohta K.
  • Publisher: Biotechnology and Genetic Engineering Reviews (UK)
• [Book] 特集「染色体サイクル」における「減数分裂の組換え開始機構」 (2007)
  • Author(s): 廣田耕志、福田智行、太田邦史
  • Total Pages: 215
  • Publisher: 羊土社
• [Remarks] Genes & Genetic Systems Award 200(日本遺伝学会論文賞)
• [Remarks] 平成19 年(2007 年) 文部科学大臣表彰科学技術賞(研究部門)
• [Remarks] プレス発表 平成20 年9 月「ガラクタ」RNA の遺伝子活性化における新しい役割
• [Remarks]
  • URL: http://www.rtokyo.acjp/public/public01_200929j.html
• [Patent(Industrial Property Rights)] 耐熱性多頻度DNA切断酵素の細胞内活性化によるゲノム再編成の誘発方法 (2008)
  • Inventor(s): 太田邦史、廣田耕志、瀬尾秀宗、柴田武彦
  • Industrial Property Rights Holder: (独)理化学研究所・(独)科学技術振興機構
  • Acquisition Date: 2008-07-25
• [Patent(Industrial Property Rights)] クロマチン再編成因子の機能改変による相同組換えの制御方法 (2008)
  • Inventor(s): 太田邦史、水野健一、柴田武彦
  • Industrial Property Rights Holder: (独)理化学研究所・(独)科学技術振興機構
  • Acquisition Date: 2008-12-26
• [Patent(Industrial Property Rights)] 体細胞相同組換えの促進方法及び特異的抗体の作製方法 (2008)
  • Inventor(s): 太田邦史、瀬尾秀宗、柴田武彦
  • Industrial Property Rights Holder: (独)理化学研究所・(独)科学技術振興機構
  • Acquisition Date: 2008-11-04
• [Patent(Industrial Property Rights)] 体細胞相同組換えの促進方法及び特異的抗体の作製方法 (2008)
  • Inventor(s): 太田邦史、瀬尾秀宗、柴田武彦
  • Industrial Property Rights Holder: (独)理化学研究所・(独)科学技術振興機構
  • Acquisition Date: 2008-04-09
  • Overseas
• [Patent(Industrial Property Rights)] 耐熱性多頻度DNA切断酵素の細胞内活性化によるゲノム再編成の誘発方法 (2008)
  • Inventor(s): 太田邦史ら
  • Industrial Property Rights Holder: 理化学研究所・JST
  • Acquisition Date: 2008-07-25
• [Patent(Industrial Property Rights)] クロマチン再編成因子の機能改変による相同組換えの制御方法 (2008)
  • Inventor(s): 太田邦史ら
  • Industrial Property Rights Holder: 理化学研究所・JST
  • Acquisition Date: 2008-12-26
• [Patent(Industrial Property Rights)] 体細胞相同組換えの促進方法及び特異的抗体の作製方法 (2008)
  • Inventor(s): 太田邦史ら
  • Industrial Property Rights Holder: 理化学研究所・JST
  • Acquisition Date: 2008-11-14