Functional analysis of reducing equivalents transfer system on the thylakoid membranes

• Project/Area Number: 19570033
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 植物生理・分子
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: MOTOHASHI Takeshi Tokyo Institute of Technology, 資源化学研究所, 資源化学研究所特別研究員 (90301952)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2008: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2007: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
• Keywords: オルガネラ / 葉緑体 / 酸化還元制御

Research Abstract

チラコイド膜を介した還元力供給過程に関連すると考えられるシステムを明らかにするため、本研究ではこれに関連することが予想されるタンパク質の抗体作成およびそれらの抗体を用いたin vitroアッセイ系の構築を目指してin vitroでの還元力伝達アッセイ実験を行った。その結果、関連することが予想されるタンパク質の抗体を用いてin vitro還元力伝達アッセイ系を行った結果、チラコイド膜を介した還元力の伝達に膜因子が関与する可能性が示唆された。

Research Products

• [Journal Article] Identification of thioredoxin targeted proteins using thioredoxin single cysteine mutant-immobilized resin Methods in Molecular Biology (2009)
  • Author(s): Ken Motohashi, Patrick G. N. Romano and Toru Hisabori
  • Journal Title: 479 Pages: 117-131
  • Peer Reviewed
• [Journal Article] Identification of thioredoxin targeted proteins using thioredoxin single cysteine mutant-immobilized resin (2009)
  • Author(s): Ken Motohashi, Patrick G. N. Romano, Toru Hisabori
  • Journal Title: Methods in Molecular Biology 479 Pages: 117-131
  • Peer Reviewed
• [Journal Article] Toru Hisabori and Tatsuru Masuda Functional analysis of Arabidpsis thaliana isoforms of the Mg-chelatase CHLI subunit (2008)
  • Author(s): Koichi Kobayashi, Nobuyoshi Mochizuki, Naho Yoshimura, Ken Motohashi
  • Journal Title: Photochemical & Photobiological Sciences7 Pages: 1188-1195
  • Peer Reviewed
• [Journal Article] Binary reducing equivalent pathways using NADPH-thioredoxin reductase and ferredoxin-thioredoxin reductase in the cyanobacterium Synechocystis sp (2008)
  • Author(s): Shoko Hishiya, Wakako Hatakeyama, Yoko Mizota, Naomi Hosoya-Matsuda, Ken Motohashi, Masahiko Ikeuchi and Toru Hisabori
  • Journal Title: strain PCC 6803 Plant and Cell Physiology 49 Pages: 11-18
  • Peer Reviewed
• [Journal Article] Functional analysis of Arabidpsis thaliana isoforms of the Mg-chelatase CHLI subunit (2008)
  • Author(s): Koichi Kobayashi, Nobuyoshi Mochizuki, Naho Yoshimura, Ken Motohashi, ToruHisabori, Tatsuru Masuda
  • Journal Title: Photochemical & Photobiological Sciences 7 Pages: 1188-1195
  • Peer Reviewed
• [Journal Article] Binary reducing equivalent pathways using NADPH-thioredoxin reductase and ferredoxin-thioredoxin reductase in the cyanobacterium Synechocystis sp. strain PCC 6803 (2008)
  • Author(s): Shoko Hishiya, Wakako Hatakeyama, Yoko Mizota, Naomi Hosoya-Matsuda, Ken Motohashi, Masahiko Ikeuchi and Toru Hisabori
  • Journal Title: Plant and Cell Physiology 49 Pages: 11-18
  • Peer Reviewed
• [Journal Article] The significance of type II and PrxQ peroxiredoxins for antioxidative stress response in the purple bacterium rhodobacter sphaeroides (2007)
  • Author(s): Masahiro Wakita, Shinji Masuda, Ken Motohashi, Toru Hisabori, Hiroyuki Ohta and Ken-ichiro Takamiya
  • Journal Title: The Journal of Biological Chemistry 282 Pages: 27792-27801
  • Peer Reviewed
• [Journal Article] The CHLI1 subunit of Arabidopsis thaliana magnesium chelatase is a target protein of the chloroplast thioredoxin (2007)
  • Author(s): Akinori Ikegami, Naho Yoshimura, Ken Motohashi, Shigekazu Takahashi, Patrick G. N. Romano, Toru Hisabori, Ken-ichiro Takamiya and Tatsuru Masuda
  • Journal Title: The Journal of Biological Chemistry 282 Pages: 19282-19291
  • Peer Reviewed
• [Journal Article] The CHLI1 subunit of Arabidopsis thaliana magnesium chelatase is a target protein of the chloroplast thioredoxin (2007)
  • Author(s): Akinori Ikegami, Naho Yoshimura, Ken Motohashi, Shigekazu Takahashi, Patrick G.N. Romano, Toru Hisabori, Ken-ichiro Takamiya and Tatsuru Masuda
  • Journal Title: The Journal of Biological Chemistry 282 Pages: 19282-19291
  • Peer Reviewed
• [Presentation] 葉緑体チラコイド膜を介したストロマから内腔への還元力伝達機構の解明 (2009)
  • Author(s): 本橋健、久堀徹
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学東山キャンパス
• [Presentation] Redox cascade through thioredoxin-family proteins in plant chloroplast (2008)
  • Author(s): Ken MOTOHASHI, Toru HISABORI
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会(BMB2008)
  • Place of Presentation: 神戸・ポートピア
• [Presentation] Redox cascade through thioredoxin-family proteins in plant chloroplast (2008)
  • Author(s): Ken MOTOHASHl, Tom HISABORI
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会(BMB2008)
  • Place of Presentation: 神戸・ポートピア
• [Presentation] Physiological significance of the thioredoxin networks in chloroplasts (2007)
  • Author(s): 久堀徹、本橋健、原怜、丸井弘嗣、池上陽紀、吉村奈穂、増田建
  • Organizer: 第30回日本分子生物学会、第80回日本生化学会合同大会(BMB2007)
  • Place of Presentation: 横浜, パシフィコ横浜
• [Presentation] Functional analysis of the luminal thioredoxin-like protein, HCF164 (2007)
  • Author(s): Ken MOTOHASHI, Masasuke YOSHIDA, Toru HISABORI
  • Organizer: The 14th International Congress of Photosynthesis
  • Place of Presentation: Glasgow, United Kingdom
• [Presentation] Physiological significance of the thioredoxin networks in chloroplasts (2007)
  • Author(s): 久堀徹、本橋健、原怜、丸井弘嗣、池上陽紀、吉村奈穂、増田建
  • Organizer: 第30回日本分子生物学会、第80回日本生化学会合同大会(BMB2007)
  • Place of Presentation: 横浜,パシフィコ横浜