Characterization and structural analysis of the plant specific kinesin encoded on the rice genome

• Project/Area Number: 19570047
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 植物生理・分子
• Research Institution: Soka University
• Principal Investigator: MARUTA Shinsaku Soka University, 工学部, 教授 (40231732)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: イネ / 分子モーター / キネシン / 植物分子

Research Abstract

We have succeeded to express several plant specific kinesins encoded on the rice genome using E.coli. expression system. Enzymatic characterization and the crystal structure of the expressed kinesins were analyzed. The results revealed that some of the kinesin have unique enzymatic properties and structure that may reflect the plant specific physiological role of the kinesins.

Research Products

• [Journal Article] Characterization of a novel rice kinesin O12 with a calponin homology domain (2011)
  • Author(s): Umezu N., Umeki N., Mitsui T., Kondo K., Maruta S.
  • Journal Title: Journal of Biochemistry. 149 Pages: 91-101
  • NAID: 10030582991
  • Peer Reviewed
• [Journal Article] Biochemical characterization of the novel rice kinesin K23 and its kinetic study using fluorescence resonance energy transfer between an intrinsic tryptophan residue and a fluorescent ATP analogue (2011)
  • Author(s): Umezu N., Hanzawa N., Yamada M.D., Kondo K., Maruta S.
  • Journal Title: Journal of Biochemistry 149 Pages: 539-550
  • NAID: 10030585182
• [Journal Article] Characterization of a novel rice kinesin O12 with a calponin homologydomain (2011)
  • Author(s): Umezu, N., Umeki, N., Mitsui, T., Kondo, K., Maruta, S.
  • Journal Title: The Journal of Biochemistry Volume: 149 Pages: 91-101
  • Peer Reviewed
• [Journal Article] Biochemical characterization of the novel rice kinesin K23 and its kinetic study using fluorescence resonance energy transfer between an intrinsic tryptophan residue and a fluorescent ATP analogue (2011)
  • Author(s): Umezu, N., Hanzawa, N., Yamada, M.D., Kondo, K., Mitsui, T., Maruta, S.
  • Journal Title: The Journal of Biochemistry Volume: 149 Pages: 539-550
  • NAID: 10030585182
  • Peer Reviewed
• [Journal Article] Crystallographic analysis reveals a unique conformation of the ADP-bound novel rice kinesin K16 (2010)
  • Author(s): Tanaka K., Umeki N., Mitsui T., Fujimoto Z., Maruta S.
  • Journal Title: Biochem Biophys Res Commun. 401 Pages: 251-256
  • Peer Reviewed
• [Journal Article] Crystallographic analysis reveals a unique conformation of the ADP-bound novel rice kinesin K16 (2010)
  • Author(s): Tanaka, K., Umeki, N., Mitsui, T., Fujimoto, Z., Maruta, S.
  • Journal Title: Biochem.and biophys.research communications Volume: 401 Pages: 251-256
  • Peer Reviewed
• [Journal Article] Kinesin-Calmodulin fusion protein as a molecular shuttle (2010)
  • Author(s): Shishido, H., Nakazato, K., Katayama, E., Chaen, S., Maruta, S.
  • Journal Title: Journal of Biochemistry 147 Pages: 213-223
  • NAID: 10027873779
  • Peer Reviewed
• [Journal Article] Conformational dynamics of loop L11 and L12 of kinesin as revealed by spin-labeling EPR (2007)
  • Author(s): Yamada, D. M., Maruta, S., Yasuda, S., Kondo, K., Maeda, H., Arata, T.
  • Journal Title: Biochemical and Biophysical Research Communications 364 Pages: 620-626
  • Peer Reviewed
• [Journal Article] Conformational dynamics of loop L11 and L12 of kinesin as revealed by spin-labeling EPR (2007)
  • Author(s): Yamada, D. M., Maruta, S., Yasuda, S., Kondo, K., Maeda, H., & Arata, T.
  • Journal Title: Biochemical and Biophysical Research Communications 364 Pages: 620-626
  • Peer Reviewed
• [Journal Article] Photocontrol of kinesin ATPase activity using an azobenzene derivative (2007)
  • Author(s): Yamada, D. M., Nakajima, Y., Maeda, H., & Maruta, S.
  • Journal Title: Journal of Biochemistry 142 Pages: 691-698
  • NAID: 10020095105
  • Peer Reviewed
• [Presentation] Preparation and polymerization of tubulin of ginkgo biloba and its interaction with rice plant kinesin (2011)
  • Author(s): Iwata S., Umezu N., Kondo K., Maruta S.
  • Organizer: 第55回米国生物物理学会
  • Place of Presentation: Baltimore, MD USA.
  • Year and Date: 2011-03-08
• [Presentation] Characterization of the plant-specific rice kinesins (2011)
  • Author(s): Umezu N., Umeki N., Hanzawa N., Kondo K., Mitsui T., Maruta S.
  • Organizer: 第55回米国生物物理学会
  • Place of Presentation: Baltimore, MD USA.
  • Year and Date: 2011-03-06
• [Presentation] Characterization of the plant-specific rice kinesins (2011)
  • Author(s): Umezu, N., Umeki, N., Hanzawa, N., Kondo, K., Mitsui, T., Maruta, S.
  • Organizer: 第55回米国生物物理学会年会
  • Place of Presentation: Baltimore, MD USA
  • Year and Date: 2011-03-06
• [Presentation] The characterization of the novel rice kinesin K23 at kinesin-7 subfamily (2010)
  • Author(s): Nozomi Umezu, Nobue Hanzawa, Kazunori Kondo, Toshiaki Mitsui, Shinsaku Maruta
  • Organizer: 第83回日本生化学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Kinetic studies on the novel rice kinesin K23 utilizing FRET between the intrinsic tryptophan residue and fluorescent ATP analogue (2010)
  • Author(s): Nozomi Umezu, Nobue Hanzawa, Kazunori Kondo, Toshiaki Mitsui, Shinsaku Maruta
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-21
• [Presentation] Preparation of microtubules derived from plant and its interaction with rice plant kinesin (2010)
  • Author(s): Seigo Iwata, Kazunori Kondo, Shinsaku Maruta
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-21
• [Presentation] 新規イネキネシンのトリプトファンと蛍光ATPアナログ間のFRETを利用した速度論的解析 (2010)
  • Author(s): 梅津のぞみ, 半澤伸枝, 近藤和典, 三ツ井敏明, 丸田晋策
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-21
• [Presentation] ANALYSIS OF THE ROLE OF UNIQUE LOOP L5 IN RICE KINESIN K16 MOTOR DOMAIN (2010)
  • Author(s): Ishikawa K., Tanaka K., Maruta S.
  • Organizer: 第54回米国生物物理学会
  • Place of Presentation: San Francisco, CA USA.
  • Year and Date: 2010-02-21
• [Presentation] ANALYSIS OF CONFORMATIONAL CHANGE OF CONVENTIONAL KINESIN CHIMERIC PROTEIN FUSED WITH GFP USING SMALL ANGLE X-RAY SOLUTION SCATTERING (2010)
  • Author(s): Tanaka, K., Sugimoto, Y., Wakabayashi, K., Maruta, S.
  • Organizer: 第54回米国生物物理学会
  • Place of Presentation: San Francisco, CA USA.
  • Year and Date: 2010-02-21
• [Presentation] Characterization of the Novel Rice Kinesin O12 which Has CH Domain (2009)
  • Author(s): Umezu N., Kondo K., Mitsui T., Maruta S.
  • Organizer: 第49回米国細胞生物学会年会
  • Place of Presentation: San Diego, CA USA.
  • Year and Date: 2009-12-08
• [Presentation] Characterization of the Novel Rice Kinesin O12 which Has CH Domain (2009)
  • Author(s): Umezu, N., Kondo, K., Mitsui, T., Maruta, S.
  • Organizer: 第49回米国細胞生物学会年会
  • Place of Presentation: San Diego, CA USA
  • Year and Date: 2009-12-08
• [Presentation] ANALYSIS OF THE ROLE OF UNIQUE LOOP L5 IN RICE KINESIN K16 MOTOR DOMAIN (2009)
  • Author(s): Ishikawa, K., Tanaka, K., Maruta, S.
  • Organizer: 第49回米国細胞生物学会年会
  • Place of Presentation: San Diego, CA USA
  • Year and Date: 2009-12-08
• [Presentation] ANALYSIS OF CONFORMATIONAL CHANGE OF CONVENTIONAL KINESIN CHIMERIC PROTEIN FUSED WITH GFP USING SMALL ANGLE X-RAY SOLUTION SCATTERING (2009)
  • Author(s): Tanaka, K., Sugimoto, Y., Wakabayashi, K., Maruta, S.
  • Organizer: 第49回米国細胞生物学会年会
  • Place of Presentation: San Diego, CA USA
  • Year and Date: 2009-12-08
• [Presentation] Characterization of the novel rice kinesin K23 (2009)
  • Author(s): Nobue Hanzawa, Nozomi Umezu, Kazunori Kondo, Toshiaki Mitsui, Shinsaku Maruta
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-31
• [Presentation] Interaction of the novel rice kineisn O12 that has the calponin homology domain with actin Kinesin Calmodulin Fusion Protein as a Molecular Shuttle (2009)
  • Author(s): Nozomi Umezu, Kazunori Kondo, Toshiaki Mitsui, Shinsaku Maruta
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-31
• [Presentation] The functional role of the unique loop L5 of kinesin (2009)
  • Author(s): Kumiko Ishikawa, Keiko Tanaka, Shinsaku Maruta
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-31
• [Presentation] Analysis of conformational change of kinesin neck-linker using using small angle X-ray solution scattering (2009)
  • Author(s): Keiko Tanaka, Yasunobu Sugimoto, Masafumi D. Yamada, Katsuzo Wakabayashi, Shinsaku Maruta
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-31
• [Presentation] Characterization of the plant rice kinesin O12 that has a calponin homology domain (2009)
  • Author(s): Nozomi Umezu, Kazunori Kondo, Toshiaki Mitsui, Shinsaku Maruta
  • Organizer: 第82回日本生化学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] Kinetic characterization of the rice kinesins using fluorescent-ATP analogue. (2009)
  • Author(s): UMEZU, N., KUBO, Y., MITSUI, T., & MARUTA, S.
  • Organizer: 第53回 米国生物物理学会年会
  • Place of Presentation: Boston, MA USA
  • Year and Date: 2009-03-01
• [Presentation] Expression and characterization of novel rice kinesin E15. (2009)
  • Author(s): Masako TSUCHIDA, Masafumi D.YAMADA, Kazunori KONDO, 丸田晋策
  • Organizer: Biophysical Society 53^<rd> Annual Meeting(Biophysical Journal Abstracts Issue : p.137a)
  • Place of Presentation: Boston USA
• [Presentation] Kinetic characterization of the rice kinesins using fluorescent-ATP analogue. (2009)
  • Author(s): Nozomi UMEZU, Yuko KUBO, Kazunori KONDO, Toshiaki MITSUI, Shinsaku MARUTA
  • Organizer: Biophysical Society 53^<rd> Annual Meeting(Biophysical Journal Abstracts Issue : p.137a)
  • Place of Presentation: Boston USA
• [Presentation] Analysis of crystal structure and solution structure of the motor domain of rice kinesin K16. (2009)
  • Author(s): Shinsaku MARUTA, Keiko TANAKA, Yoko KUBO, Toshiaki MITSUI, Zui FUJIMOTO
  • Organizer: Biophysical Society 53^<rd> Annual Meeting(Biophysical Journal Abstracts Issue : p.137a)
  • Place of Presentation: Boston USA
• [Presentation] Analysis fo conformational change of novel rice kinesin K16 using small angle X-ray solution scattering and EPR. (2009)
  • Author(s): Yuko KUBO, Satoshi YASUDA, Yasunobu SUGIMOTO, Masafumi YAMADA, Toshiaki ARATA, Katsuzo WAKABAYASHI, Shinsaku MARUTA
  • Organizer: Biophysical Society 53^<rd> Annual Meeting(Biophysical Journal Abstracts Issue : p.138a)
  • Place of Presentation: Boston USA
• [Presentation] Expression and characterization of novel rice kinesin E15 (2009)
  • Author(s): Masako TSUCHIDA, Masafumi D. YAMADA, Kazunori KONDO, Shinsaku MARUTA
  • Organizer: Biophysical Society 53^<rd> Annual Meeting
  • Place of Presentation: Boston USA
• [Presentation] Kinetic characterization of the rice kinesins using fluorescent-ATP analogue (2009)
  • Author(s): Nozomi UMEZU, Yuko KUBO, Kazunori KONDO, Toshiaki MITSUI, Shinsaku MARUTA
  • Organizer: Biophysical Society 53^<rd> Annual Meeting
  • Place of Presentation: Boston USA
• [Presentation] Analysis of crystal structure and solution structure of the motor domain of rice kinesin K16. Biophysical Society 53^<rd> Annual Meeting (2009)
  • Author(s): Shinsaku MARUTA, Keiko TANAKA, Yoko KUBO, Toshiaki MITSUI, Zui FUJIMOTO
  • Place of Presentation: Boston USA
• [Presentation] Analysis of conformational change of novel rice kinesin K16 using small angle X-ray solution scattering and EPR (2009)
  • Author(s): Yuko KUBO, Satoshi YASUDA, Yasunobu SUGIMOTO, Masafumi YAMADA, Toshiaki ARATA, Katsuzo WAKABAYASHI, Shinsaku MARUTA
  • Organizer: Biophysical Society 53^<rd> Annual Meeting
  • Place of Presentation: Boston USA
• [Presentation] Kinetic characterization of the novel rice kinesin L05 and L04 using fluorescent ATP analogue. (2008)
  • Author(s): Nozomi UMEZU, Yuko KUBO, Toshiaki MITSUI, Shinsaku MARUTA
  • Organizer: 第46回日本生物物理学会(同学会要旨集 p.S101)
  • Place of Presentation: 福岡
• [Presentation] Analysis of crystal structure of the motor domain of rice kinesin K16 and comparison with the structures of other related kinesin. (2008)
  • Author(s): Keiko TANAKA, Toshiaki MITSUI, Zui FUJIMOTO, Shinsaku MARUTA
  • Organizer: 第46回日本生物物理学会(同学会要旨集 p.S102)
  • Place of Presentation: 福岡
• [Presentation] Preparation and characterization of novel rice kinesin E15. (2008)
  • Author(s): Masako TSUCHIDA, Masafumi D. YAMADA, Kazunori KONDO, Shinsaku MARUTA
  • Organizer: 第46回日本生物物理学会(同学会要旨集 p.S102)
  • Place of Presentation: 福岡
• [Presentation] Analysis fo conformational change of novel rice kinesin K16 using small angle X-ray scattering and FRET. (2008)
  • Author(s): Yuko KUBO, Masafumi D.YAMADA, Hideki SHISHIDO, Yasunobu SUGIMOTO, Katsuzo WAKABAYASHI, Shinsaku MARUTA
  • Organizer: 第46回日本生物物理学会(同学会要旨集 p.S103)
  • Place of Presentation: 福岡
• [Presentation] Analysis of conformational change of novel rice kinesin K16 using small angle X-ray solution scattering. (2008)
  • Author(s): Yuko KUBO, Masafumi D.YAMADA, Yasunobu SUGIMOTO, Katsuzo WAKABAYASHI, Shinsaku MARUTA
  • Organizer: 2^<nd> International symposium on Bio-nanosystems(Abstract : p.67)
  • Place of Presentation: Tokyo
• [Presentation] Kinetic characterization of the novel rice kinesin L05 and L04 using fluorescent ATP analogue (2008)
  • Author(s): Nozomi UMEZU, Yuko KUBO, Toshiaki MITSUI, Shinsaku MARUTA
  • Organizer: 第46回日本生物物理学会
  • Place of Presentation: 福岡
• [Presentation] Preparation and characterization of novel rice kinesin E15 (2008)
  • Author(s): Masako TSUCHIDA, Masafumi D. YAMADA, Kazunori KONDO, Shinsaku MARUTA
  • Organizer: 第46回日本生物物理学会
  • Place of Presentation: 福岡
• [Presentation] Analysis fo conformational change of novel rice kinesin K16 using small angle X-ray scattering and FRET (2008)
  • Author(s): Yuko KUBO, Masafumi D. YAMADA, Hideki SHISHIDO, Yasunobu SUGIMOTO, Katsuzo WAKABAYASHI, Shinsaku MARUTA
  • Organizer: 第46回日本生物物理学会
  • Place of Presentation: 福岡
• [Presentation] Analysis of conformational change of novel rice kinesin K16 using small angle X-ray solution scattering (2008)
  • Author(s): Yuko KUBO, Masafumi D. YAMADA, Yasunobu SUGIMOTO, Katsuzo WAKABAYASHI, Shinsaku MARUTA
  • Organizer: 2^<nd> International symposium on Bio-nanosystems
  • Place of Presentation: Tokyo
• [Presentation] X線小角散乱を用いた新規イネキネシンK16の構造変化の解析 (2007)
  • Author(s): 久保優子、山田正文、杉本泰伸、若林克三、丸田晋策
  • Organizer: 第45回日本生物物理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-22
• [Presentation] 結晶構造解析で明らかになったイネキネシンK16のネックリンカーの特徴的コンホメーション (2007)
  • Author(s): 丸田晋策、梅木伸久、三ツ井敏明、藤本瑞
  • Organizer: 第45回日本生物物理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-22
• [Presentation] 蛍光標識ATPアナログを用いた新規イネキネシンのK16のATPase kinetic pathwayの観察 (2007)
  • Author(s): 土田雅子、山田正文、吉村栄子、近藤和典、丸田晋策
  • Organizer: 第45回日本生物物理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-22
• [Presentation] 蛍光標識ATPアナログを用いたイネキネシン012の生化学的解析 (2007)
  • Author(s): 梅津のぞみ、梅木伸久、近藤和典、三ツ井敏明、丸田晋策
  • Organizer: 第45回日本生物物理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-22
• [Presentation] X線小角散乱を用いた新規イネキネシンK16の構造変化の解析 (2007)
  • Author(s): 久保優子, 山田正文, 杉本泰伸, 若林克三, 丸田晋策
  • Organizer: 第45回 日本生物物理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-22
• [Presentation] 藤本瑞結晶構造解析で明らかになったイネキネシンK16のネックリンカーの特徴的コンホメーション (2007)
  • Author(s): 丸田晋策, 梅木伸久, 三ツ井敏明
  • Organizer: 第45回 日本生物物
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-22
• [Presentation] 蛍光標識ATPアナログを用いた新規イネキネシンのK16の ATPase kinetic pathwa の観察 (2007)
  • Author(s): 土田雅子, 山田正文, 吉村栄子, 近藤和, 丸田晋策
  • Organizer: 第45回 日本生物物理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-22
• [Presentation] 蛍光標識ATPアナログを用いたイネキネシン012の生化学的解析 (2007)
  • Author(s): 梅津のぞみ, 梅木伸久, 近藤和典, 三ツ井敏明, 丸田晋策
  • Organizer: 第45回 日本生物物理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-22
• [Presentation] イネキネシンK16モータードメインの結晶構造 (2007)
  • Author(s): 丸田晋策、梅木伸久、三ツ井敏明、藤本瑞
  • Organizer: 第80回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Presentation] イネキネシンK16モータードメインの結晶構造 (2007)
  • Author(s): 丸田晋策, 梅木伸久, 三ツ井敏明, 藤本瑞
  • Organizer: 第80回 日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Presentation] Characterization of the ATPase kinetic pathway of the novel rice kinesin K16 (2007)
  • Author(s): Tsuchida, M., Yamada, M., Yoshimura, E., Kondo, K., Maruta, S.
  • Organizer: 第47回 米国細胞生物学会年会
  • Place of Presentation: Washington, DC USA
  • Year and Date: 2007-12-02
• [Presentation] Crystallographic Study on the Motor Domain of Rice Kinesin K16 Reveals a Novel Conformation of Neck-linker (2007)
  • Author(s): Maruta, S., Umeki, N., Mitsui, T., Fujimoto, Z.
  • Organizer: 第47回 米国細胞生物学会年会
  • Place of Presentation: Washington, DC USA
  • Year and Date: 2007-12-02
• [Presentation] Analysis of conformational change of novel rice kinesin K16 using small angle X-ray solution scattering
  • Author(s): Kubo, Y., Yamada, M. D., Sugimoto, Y., Wakabayashi, K., Maruta, S.
• [Remarks] ホームページ
  • URL: http://www.t.soka.ac.jp/maruta-lab/