Studies on early imbibition responses in Arabidopsis seeds

• Project/Area Number: 19570051
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 植物生理・分子
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: NANBARA Eiji The Institute of Physical and Chemical Research, 生長制御研究グループ, 客員主管研究員 (90271626)
• Co-Investigator(Kenkyū-buntansha): TATEMATSU Kiyoshi 独立行政法人理化学研究所, 適応制御研究チーム, 研究員 (00373324)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 種子 / 吸水 / シロイヌナズナ / マイクロアレイ / 遺伝子発現 / 植物ホルモン / 種子吸水

Research Abstract

種子吸水時の分子メカニズムにかんする研究をおこなった。種子吸水と発芽/休眠の過程を区別するために、シロイヌナズナの休眠性が高い種子(Cvi系統)と低い系統(Col系統)の比較解析をおこなった。マイクロアレイによる遺伝子発現解析の結果、Cvi系統でもCol系統でも吸水後3時間で顕著な遺伝子発現変動を見いだした。また、シクロへキシミドを用いた研究から、これら遺伝子発現は、種子に蓄えられた転写因子によることを示した。

Research Products

• [Journal Article] CHOTTO1, a double AP2 domain protein of Arabidopsis thaliana, regulates germination and seedling growth under excess supply of glucose and nitrate (2009)
  • Author(s): Yamagishi K, Tatematsu K, Yano R, Preston J, Kitamura S, Takahashi H, McCourt P, Kamiya Y, Nambara E
  • Journal Title: Plant Cell Physiology 50 Pages: 330-340
  • NAID: 10027337592
  • Peer Reviewed
• [Journal Article] The Arabidopsis abscisic acid catabolism gene CYP707A2 plays a key role in nitrate control of seed dormancy (2009)
  • Author(s): Matakiadis T, Alboresi A, Jikumaru Y, Tatematsu K, Pichon O, Renou J-P, Sotta B, Kamiya Y, Nambara E, Troung H-N.
  • Journal Title: Plant Physiology 149 Pages: 949-960
  • Peer Reviewed
• [Journal Article] The GA-signaling repressor RGL2 inhibits Arabidopsis seed germination by stimulating ABA synthesis and ABI5 activity (2008)
  • Author(s): Piskurewicz U, Jikumaru Y, Kinoshita N, Nambara E, Kamiya Y, Lopez-Molina L.
  • Journal Title: Plant Cell 20 Pages: 2729-2745
  • Peer Reviewed
• [Journal Article] AtTCP14 regulates embryonic growth potential in Aranbidopsis thaliania. (2008)
  • Author(s): Tatematsu K, Nakabayashi K, Kamiya Y, and Nambara E.
  • Journal Title: Plant Journal 53 Pages: 42-52
  • Peer Reviewed
• [Journal Article] High temperature-induced ABA biosynthesis and its role in the inhibition of GA action in Arabidopsis seeds. (2008)
  • Author(s): Toh S, Imamura A, Watanabe A, Nakabayashi K, Okamoto M, Jikumaru Y, Hanada A, Aso Y, Ishiyama K, Tamura N, Iuchi S, Kobayashi M, Yamaguchi S, Kamiya Y, Nambara E, and Kawakami N.
  • Journal Title: Plant Physiology (In press)
  • Peer Reviewed
• [Journal Article] Phytochrome-and gibberellin-mediated regulation of abscisic acid metabolism during germination of photoblastic lettuce seeds. (2008)
  • Author(s): Sawada Y, Aoki M, Nakaminami K, Mitsuhashi W, Tatematsu K, Kushiro T, Koshiba T, Kamiya Y, Inoue Y, Nambara E.and Toyomasu T.
  • Journal Title: Plant Physiology (In press)
  • Peer Reviewed
• [Presentation] STUDIES ON IMBIBITION-INDUCED GENE EXPRESSION IN DORMANT AND NON-DORMANT ARABIDOPSIS SEEDS: THE EFFECT OF CYCLOHEXIMDE ON EARLY INDUCTION OF GENE EXPRESSION (2009)
  • Author(s): Mitsuhiro Kimura and Eiji Nambara
  • Organizer: 4^<th> International workshop on plant dormancy
  • Place of Presentation: Fargo, ND, US
• [Presentation] シロイヌナズナ種子吸水時の遺伝子発現解析 (2008)
  • Author(s): 立松圭、Jeremy Preston、菅野裕理、藤茂雄、川上直人、神谷勇治、南原英司
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 札幌
• [Presentation] COMPARATIVE STUDIES ON NON-DORMANT COL AND DORMANT CVI SEEDS: COMMON AND POLYMORPHIC REGULATIONS ON MOLECULAR MECHANISM OF SEED IMBIBITION IN ARABIDOPSIS (2008)
  • Author(s): Mitsuhiro Kimura, Jeremy Preston, Kiyoshi Tatematsu, Yuri Kanno, Shigeo Toh, Naoto Kawakami, Yuji Kamiy, and Eiji Nambara
  • Organizer: International meeting on Arabidopsis research
  • Place of Presentation: Montreal, Canada
• [Presentation] シロイヌナズナ初期吸水における遺伝子発現 (2008)
  • Author(s): 立松 圭
  • Organizer: 日本植物生理学会
  • Place of Presentation: 札幌コンベンションホール
• [Book] 種子科学とバイオテクノロジー (2009)
  • Author(s): 矢野亮一, 南原英司
  • Total Pages: 7
  • Publisher: 学会出版センター