A novel function of myosin-binding protein-C
| Project/Area Number |
19570067
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | Chiba University |
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Principal Investigator |
SATO Naruki Chiba University, 大学院・融合科学研究科, 講師 (40261896)
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| Co-Investigator(Kenkyū-buntansha) |
山本 啓一 千葉大学, 大学院・融合科学研究科, 教授 (70053361)
伊藤 光二 千葉大学, 大学院・融合科学研究科, 講師 (50302526)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAMOTO Keiichi 千葉大学, 大学院・融合科学研究科, 教授 (70053361)
ITO Kohji 千葉大学, 大学院・理学研究科, 准教授 (50302526)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | C-タンパク質 / 筋収縮 / ミオシン / アクチン / トロポニン / 筋肉 / MyBP-C / 骨格筋 / 心筋 / MyBp-C / C-タンパク |
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| Research Abstract |
Myosin-binding protein-C (MyBP-C/C-protein) is one of the major myosin-binding proteins in vertebrate striated muscles and consists of three isoforms. It has been demonstrated that MyBP-C interacts with LMM and connectin/titin at the C-terminus, and with the S2 segments of myosin at the N-terminus. It is also known that MyBP-C binds to actin filaments and modulates the actin-activated ATPase of myosin. However, actin-binding region of MyBP-C has not yet been fully clarified. In this study, we prepared recombinant N-terminal fragments of cardiac, fast and slow MyBP-C in an E.coli expression system. Co-sedimentation assay showed that each fragment bound to F-actin. In addition, the fast and the cardiac fragment of MyBP-C inhibited the actomyosin ATPase activity. It suggests that MyBP-C regulates actin-myosin interaction during muscle contraction at the N-terminal side.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Identification of cardiac-specific myosin light chain kinase2008
Author(s)
Chan JY, Takeda M, Briggs LE, Graham ML, Lu JT, Horikoshi N, Weinberg EO, Aoki H, Sato N, Chien KR, Kasahara H
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Journal Title
Circulation Research 102
Pages: 571-580
Related Report
Peer Reviewed
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