Cross-talk of Ca^2+ signal on acrosome reaction in starfish

• Project/Area Number: 19570074
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: Keio University
• Principal Investigator: MATSUMOTO Midori Keio University, 理工学部, 准教授 (00211574)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Kazuya 慶應義塾大学, 理工学部, 助教 (50360110)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ヒトデ / 受精 / 先体反応 / 卵外被分子 / 精子膜ラフト / Caイオン / 精子運動性

Research Abstract

ヒトデの先体反応について、精子内のCa^2+の上昇を促す2種類の機構(ARISとasterosapのシグナル伝達機構)が起こす単独作用の時とは全く異なる協調的なCa2+の上昇機構に焦点をあててこれを解明することにある。未だ同定できていないARIS受容体をARIS活性糖鎖であるFr.1に対するアフィニティークロマトグラフを用いて、精子膜画分よりFr.1特異的に結合性を持つ41kDの受容体候補タンパク質が見出した。さらに、その受容体候補タンパク質の精子膜ラフトへの局在を明らかにした。

Research Products

• [Journal Article] Hideaki Moriyama Identification of guanylate cyclases and related signa ling proteins in sperm tail from sea stars by mass spectrometry (2008)
  • Author(s): Mia Nakachi, Motonori Hoshi, Midori Matsumoto
  • Journal Title: Marine Biotechnology 10 Pages: 564-571
  • Peer Reviewed
• [Journal Article] Motonori Hoshi The concerted regulation of acrosome reaction in starfish by intracellular cGMP, pH, cAMP, and Ca^<2+> levels International (2008)
  • Author(s): Midori Matsumoto, Osamu Kawase, M. Sadiqul Islam, Masahiro Naruse, Shin-nosuke Watanabe
  • Journal Title: Journal of Developmental Biology 52 Pages: 523-526
  • Peer Reviewed
• [Journal Article] Egg and sperm recognition syst ems during fertilization Development (2008)
  • Author(s): Noritaka Hirohashi, Noriko Kamei, Hideo Kubo, Hitoshi Sawada, Midori Matsumoto and Motonori Hoshi
  • Journal Title: Growth and Differentiation 50 Pages: 221-238
  • Peer Reviewed
• [Journal Article] Conserved sequences of sperm-activating peptide and its receptor throughout evolution despite speciation in the sea star Asterias amurensis and closely related species (2008)
  • Author(s): Mia Nakachi, Motonori Hoshi, Midori Matsumoto, Hideaki Moriyama
  • Journal Title: Zygote 16 Pages: 1-10
  • Peer Reviewed
• [Journal Article] Identification of guanylate cyclases and related signaling proteins in sperm tail from sea stars by mass spectrometry (2008)
  • Author(s): Mia Nakachi, Motonori Hoshi, Midori Matsumoto, Hideaki Moriyama
  • Journal Title: Marine Biotechnology 10 Pages: 564-571
  • Peer Reviewed
• [Journal Article] The concerted regulation of acrosome reaction in starfish by intracellular cGMP, pH, cAMP, and Ca^<2+> levels (2008)
  • Author(s): Midori Matsumoto, Osamu Kawase, M. Sadiqul Islam, Masahiro Naruse, Shin-nosuke Watanabe, Motonori Hoshi
  • Journal Title: International Journal of Developmental Biology 52 Pages: 523-526
  • Peer Reviewed
• [Journal Article] Egg and sperm recognition systems during fertilization (2008)
  • Author(s): Noritaka Hirohashi, Noriko Kamei, Hideo Kubo, Hitoshi Sawada, Midori Matsumoto, Motonori Hoshi
  • Journal Title: Development, Growth and Differentiation 50
  • NAID: 10024400182
  • Peer Reviewed
• [Journal Article] Conserved sequences of sperm-activating peptide and its receptor throughout evolution despite speciation in the sea star Asterias amurensis and closely related species (2008)
  • Author(s): Mia Nakachi
  • Journal Title: Zygote (In press)
  • Peer Reviewed
• [Journal Article] Identification of guanylate cyclases and related signaling proteins insperm tail from sea stars by mass spectrometry (2008)
  • Author(s): Mia Nakachi
  • Journal Title: Marine Biotechnology (In press)
  • Peer Reviewed
• [Journal Article] Egg and sperm recognition systems during fertilization (2008)
  • Author(s): Noritaka Hirohashi,
  • Journal Title: Dev. Growth and Differ (In press)
• [Journal Article] Hideaki Moriyama Conserved sequences of sperm-activating peptide and its receptor throughout evolution despite speciation in the sea star (2007)
  • Author(s): Mia Nakachi, Motonori Hoshi, Midori Matsumoto
  • Journal Title: Asterias amurensis and closely related species Zygote 16 Pages: 1-10
• [Presentation] 先体反応誘起物質ARISの高次構造解析 (2009)
  • Author(s): 松鹿弥生, 成瀬正啓, 松本緑, キヒトデ
  • Organizer: 日本動物学会関東支部第61回大会
  • Place of Presentation: 埼玉
  • Year and Date: 2009-03-20
• [Presentation] マボヤ体腔細胞のアロ認識機構解明を目指して-フェノールオキシダーゼ放出誘導抗体 (2009)
  • Author(s): 岡田泰三、宮川一志、久保英夫、松本緑
  • Organizer: 日本動物学会関東支部第61回大会
  • Place of Presentation: 埼玉
  • Year and Date: 2009-03-20
• [Presentation] キヒトデ先体反応誘起物質ARISの高次構造解析 (2009)
  • Author(s): 松鹿弥生 成瀬正啓 松本緑
  • Organizer: 日本動物学会関東支部第61回大会
  • Place of Presentation: 埼玉
  • Year and Date: 2009-03-20
• [Presentation] Conserved role of ARIS ; Acorosome Reaction-Inducing Substancein the echinodermata (2009)
  • Author(s): Masahiro Naruse, Hiroshi Sakaya, Motonori Hoshi, Midori Matsumoto
  • Organizer: 13th International Echinoderm Conference
  • Place of Presentation: Tasmania, Australia
  • Year and Date: 2009-01-08
• [Presentation] Masahiro Naruse and Motonori Hoshi The concerted regulation of acrosome reaction in starfish by intracellular pH (2009)
  • Author(s): Midori Matsumoto, Osamu Kawase, Sadiqul MD Islam
  • Organizer: cAMP and Ca^<2+> level 13th International Echinoderm Conference
  • Place of Presentation: Tasmania, Australia
  • Year and Date: 2009-01-06
• [Presentation] Conserved role of ARIS (2009)
  • Author(s): Masahiro Naruse, Hiroshi Sakaya, Motonori Hoshi, Midori Matsumoto
  • Organizer: Acorosome Reaction-Inducing Substance in the echinodermata 13th International Echinoderm Conference
  • Place of Presentation: Tasmania, Australia
  • Year and Date: 2009-01-06
• [Presentation] The concerted regulation of acrosome reaction in starfish by intracellular pH, cAMP and Ca^<2+> level (2009)
  • Author(s): Midori Matsumoto, Osamu Kawase, Sadiqul MD Islam, Masahiro Naruse, Motonori Hoshi
  • Organizer: 13th International Echinoderm Conference
  • Place of Presentation: Tasmania, Australia
  • Year and Date: 2009-01-06
• [Presentation] キヒトデ先体反応誘起物質ARISタンパク質領域の同定 (2008)
  • Author(s): 成瀬正啓、石川里歩、昌谷洋、星元紀、松本緑
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡
  • Year and Date: 2008-09-06
• [Presentation] マボヤ体腔細胞の同種異個体認識反応における自己マーカー分子の探索 (2008)
  • Author(s): 高橋未来、神林祐輔、江間正起、松本緑
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡
  • Year and Date: 2008-09-05
• [Presentation] ヒトデ類における卵外被プロテオグリカンARISの共通性 (2008)
  • Author(s): 昌谷洋、成瀬正啓、松本緑
  • Organizer: FCCA若手フォーラム2008
  • Place of Presentation: つくば
  • Year and Date: 2008-08-21
• [Presentation] 体を守る防衛ライン-異物センシングシステム- (2008)
  • Author(s): 松本緑
  • Organizer: 次世代センシングシステム技術分科会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-25
• [Presentation] マボヤ体腔細胞Contact Reactionにおける液胞放出とフェノールオキシダーゼ活性の関係 (2007)
  • Author(s): 宮川一志、竹田典代、星元紀、松本緑
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前
  • Year and Date: 2007-09-22
• [Presentation] 中島陽子タマクラゲのヒドラ神経ペプチドによる放卵誘起機構 (2007)
  • Author(s): 竹田典代、出口竜作、藤澤敏孝、小泉修、松本緑
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 松江
  • Year and Date: 2007-09-20
• [Presentation] 精子先体反応誘起物質(ARIS)受容体の精子膜上での動態 (2007)
  • Author(s): 成瀬正啓, 松本緑, 星元紀, キヒトデ
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前
  • Year and Date: 2007-09-20
• [Presentation] トデ胚の形態形成における間充織細胞の動態 (2007)
  • Author(s): 濱中玄、松本緑、金子洋之 イトマキヒ
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前
  • Year and Date: 2007-09-20
• [Presentation] Analysis of the acrosome reaction-inducing substance receptor on the sperm surface membrane in Asterias amurensis Gordon Conference (2007)
  • Author(s): Masahiro Naruse, Motonori Hoshi, Midori Matsumoto
  • Organizer: Fertilization and Activation of Development
  • Place of Presentation: Holderness, USA
  • Year and Date: 2007-07-17
• [Book] Recognition mechanism of egg and sperm by sugar chains Experimental Glycoscience Glycobiology 谷口直之編 (2008)
  • Author(s): Midori Matsumoto
  • Publisher: シュプリンガー・ジャパン社
• [Book] 天然物化学-海洋生物編-山村庄亮ら編 (2008)
  • Author(s): 松本緑
  • Publisher: アイピーシー2. 6海洋生物の精子活性化、走化性と先体反応