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Structure and function of mammalian egg zona pellucida : are the sugarchains really essential for zona functions

Research Project

Project/Area Number 19570101
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionChiba University

Principal Investigator

YONEZAWA Naoto  Chiba University, 大学院・理学研究科, 准教授 (80212314)

Co-Investigator(Kenkyū-buntansha) YANAGIDA Mitsuaki  順天堂大学, 医学研究科, 准教授 (80365569)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords糖タンパク質 / 受精 / 細胞外マトリックス / 透明帯 / 糖たんぱく質
Research Abstract

卵子透明帯の構成糖タンパク質は動物種によって異なる。マウス透明帯はZP1, ZP2, ZP3の3成分からなり, ZP3が単独で精子結合活性を示す。ウシ卵子透明帯はブタと同じくZP2, ZP3, ZP4の3成分からなる。本研究でバキュロウイルス-Sf9細胞発現系を構築することにより, ウシの場合, ZP3単独では精子結合活性を示さずZP3/ZP4複合体が活性を示すことをあきらかにした。よって, ウシとブタにおいて透明帯の精子認識機構は類似していることが示唆された。ブタの場合, ZP3/ZP4複合体のZP4の糖鎖が精子結合に関わることがわかった。ブタZP3ならびにZP4のジスルフィド結合パターンは先に報告されたマウスなど他の動物種のものとは異なっていた。このパターンの違いと精子認識機構との関連性解明は今後の課題である。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (16 results)

All 2008 2007

All Journal Article (10 results) (of which Peer Reviewed: 8 results) Presentation (6 results)

  • [Journal Article] Disulfide linkage patterns of pig zona pellucida glycoproteins ZP3 and ZP42008

    • Author(s)
      Saeko Kanai, Tetsushi Kitayama, Naoto Yonezawa, Yoriko Sawano, Masaru Tanokura, Minoru Nakano
    • Journal Title

      Molecular Reproduction and Development 75

      Pages: 847-856

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A D19S433 primer binding site mutation and the frequency in Japanese of the silent allele it causes2008

    • Author(s)
      Natsuko Mizuno, Tetsushi Kitayama, Koji Fuji, Hiroaki Nakahara, Kanako Yoshida, Kazumasa Sekiguchi, ,NaotoYonezawa, Minoru Nakano, Kentaro Kasai
    • Journal Title

      Journal of Forensic Sciences 53

      Pages: 1068-1073

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structure of calcium-bound human S100A13 at pH 7.5 at 1.8 A resolution2008

    • Author(s)
      Fabiana Lica Imai, Koji Nagata, Naoto Yonezawa, Minoru Nakano, Masaru Tanokura
    • Journal Title

      Acta Crystallogr Sect F Struct Biol Cryst Commun 64

      Pages: 70-76

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Disulfide linkage patternsof pig zona pellucida glycoproteins ZP3 and ZP42008

    • Author(s)
      Saeko Kanai
    • Journal Title

      Molecular Reproduction and Development 75

      Pages: 847-856

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A D19S433 primer binding site mutation and the frequency in Japanese of the silent allele it causes2008

    • Author(s)
      Natsuko Mizuno
    • Journal Title

      Journal of Forensic Sciences 53

      Pages: 1068-1073

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure of calcium-bound human S100A13 at pH7.5at1.8 A resolution2008

    • Author(s)
      Fabiana Lica Imai
    • Journal Title

      Acta Crystallogr Sect F Struct Biol Cryst Commun 64

      Pages: 70-76

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recombinant bovine zona pellucida glycoproteins ZP3 and ZP4 coexpressedin Sf9 cells form a sperm-binding active hetero-complex2007

    • Author(s)
      Saeko Kanai, Naoto Yonezawa, Yuichiro Ishii, Masaru Tanokura, Minoru Nakano
    • Journal Title

      FEBS Journal 274

      Pages: 5390-5405

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural significance of N-glycans of the zona pellucida on species-selective recognition of spermatozoa between pig and cattle2007

    • Author(s)
      Naoto Yonezawa, Saeko Kanai, Minoru Nakano
    • Journal Title

      Society of Reproduction and Fertilitysupplement 63

      Pages: 217-228

    • Related Report
      2008 Final Research Report
  • [Journal Article] Recombinant bovine zona pellucida glycoproteins ZP3 and ZP4 coexpressed in Sf9cells form a spelm-binding active hetero-complex2007

    • Author(s)
      Saeko Kanai
    • Journal Title

      FEBS Joumal 274

      Pages: 5390-5405

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structulal significance of N-glycans of the zona pelluucida on species-selective recognition of spermatozoa between pig and cattle2007

    • Author(s)
      Naoto Yonezawa
    • Journal Title

      Society of Reproduction and Fertility supplement 63

      Pages: 217-228

    • Related Report
      2007 Annual Research Report
  • [Presentation] ウシ卵子透明帯糖タンパク質ZP3とZP4の相互作用部位2008

    • Author(s)
      児島さゆり
    • Organizer
      第81回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-11
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] ウシ妊娠関連糖タンパク質(PAG)1のN結合型糖鎖構造2008

    • Author(s)
      尾崎恵璃
    • Organizer
      第81回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Carbohydrate chains linked to ZP4 are responsible for thesperm binding activity of ZP3/ZP4 complex in pig2008

    • Author(s)
      Ayumi Hamano
    • Organizer
      第60回日本細胞生物学会大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2008-06-30
    • Related Report
      2008 Final Research Report
  • [Presentation] Carbohydrate chains linked to ZP4 are responsible for the sperm binding activity of ZP/Zp4 complex in pig2008

    • Author(s)
      Ayumi Hamano
    • Organizer
      第60回日本細胞生物学会大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2008-06-30
    • Related Report
      2008 Annual Research Report
  • [Presentation] Studies on the interaction sites between ZP domains of zona pellucida glycoproteins2007

    • Author(s)
      Saeko Kanai
    • Organizer
      第80回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-12-12
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] 卵子透明帯糖タンパク質ZP3とZP4の複合体形成がウシ精子・卵子間の結合に重要である2007

    • Author(s)
      金井咲園子
    • Organizer
      第59回日本細胞生物学会大会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2007-05-28
    • Related Report
      2008 Final Research Report 2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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