Structure and function relationships of higher plant glutamine synthetase
| Project/Area Number |
19570105
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Structural biochemistry
|
|---|
| Research Institution | University of Yamanashi (2008)
Osaka University (2007) |
|---|
Principal Investigator |
KUSUNOKI Masami University of Yamanashi, 大学院・医学工学総合研究部, 教授 (90135749)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HASE Toshiharu 大阪大学, たんぱく質研究所, 教授 (00127276)
|
|---|
| Project Period (FY) |
2007 – 2008
|
| Project Status |
Completed (Fiscal Year 2008)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | X線 / 結晶 / アンモニア同化 / 植物生理学 / 構造生物学 / グルタミン合成酵素 / 立体構造 / 窒素代謝 / 酵素 / 植物 |
|---|
| Research Abstract |
植物のグルタミン合成酵素(GS)は、細胞質局在型GS1とプラスチド局在型GS2が存在し、GS1は多重遺伝子族によりコードされている。
GS1a His249Ala/MetSox-P/ADP, GS1a WT/PPT/AMPPNP の2種類の結晶解析を行った。前者から、His249の側鎖と基質の水素結合が基質認識に重要な役割を担っていること、後者からPPTのリン酸化前とリン酸化後で酵素側の構造変化が無いことも判明した。
|
Report
(3 results)
Research Products
(16 results)
-
-
[Journal Article] Structural Basis for Substrate Recognition and Hydrolysis by Mouse Carnosinase CN22008
Author(s)
Unno, H., Yamashita, H., Ujita, S., Okumura, N., Otani, H., Okumura, A., Nagai, K. and Kusunoki, M
-
Journal Title
J. Biol.Chem. 283
Pages: 27289-27299
Related Report
Peer Reviewed
-
-
-
[Journal Article] C-Type Lectin-like Carbohydrate-Recognition of the Hemolytic Lectin CEL-III Containing Ricin-type β-Trefoil Folds2007
Author(s)
Hatakeyama, T., Unno, H., Kouzuma, Y., Uchida, T., Eto, S., Hidemura, H., Kato, N., Yonekura, M. and Kusunoki, M.
-
Journal Title
J. Biol. Chem. 282
Pages: 37826-37835
Related Report
Peer Reviewed
-
[Journal Article] Structural and mutational studies of anthocyanin malonyltransferases establish the features of BAHD enzyme catalysis2007
Author(s)
Unno, H., Ichimaida, F., Suzuki, H., Takahashi, S., Tanaka, Y., Saito, A., Nishino, T., Kusunoki, M. and Nakayama, T.
-
Journal Title
Journal of Biological Chemistry Vol. 282
Pages: 15182-15822
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
