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Recognition mechanism of Lys63-linked ubiquitin chain involved in transcription regulation and DNA repair

Research Project

Project/Area Number 19570116
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionHigh Energy Accelerator Research Organization

Principal Investigator

KAWASAKI Masato  High Energy Accelerator Research Organization, 物質構造科学研究所, 助教 (00342600)

Research Collaborator WAKATSUKI Soichi  高エネルギー加速器研究機構, 物質構造科学研究所, 教授 (00332114)
KATO Ryuichi  高エネルギー加速器研究機構, 物質構造科学研究所, 准教授 (50240833)
IGARASHI Noriyuki  高エネルギー加速器研究機構, 物質構造科学研究所, 准教授 (80300672)
HIRAKI Masahiko  高エネルギー加速器研究機構, 物質構造科学研究所, 研究機関講師 (20282676)
MATSUGAKI Naohiro  高エネルギー加速器研究機構, 物質構造科学研究所, 助教 (50342598)
YAMADA Yusuke  高エネルギー加速器研究機構, 物質構造科学研究所, 助教 (20391708)
SUZUKI Nobuhiro  高エネルギー加速器研究機構, 物質構造科学研究所, 研究員
RAHIGHI Simin  総合研究大学, 大学院, 大学院生
ROHAIM Ahmed  総合研究大学, 大学院, 大学院生
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
KeywordsX線結晶解析 / ユビキチン / 転写制御 / DNA修復 / 分子認識 / X線結晶析
Research Abstract

NF-κBは、免疫、炎症、抗アポトーシスなどに関わる様々な遺伝子の転写を活性化する転写因子であり、NEMOにより活性化される。NEMOはLys63結合ユビキチン鎖と結合すると報告されていたが、意外なことにユビキチンが直列につながったタンデムユビキチン鎖により強く結合してNF-κBを活性化することが判明した。タンデムユビキチンとNEMOの複合体の結晶構造解析の結果、NEMOはコイルドコイル二量体の両面で2分子のタンデムユビキチンを対称的に結合しており、NEMOによるユビキチン鎖の選択的認識機構が明らかになった。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (18 results)

All 2009 2008 2007 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (12 results) Remarks (4 results)

  • [Journal Article] Specific Recognition of Linear Ubiquitin Chains by NEMO Is Important for NF- κ B Activation2009

    • Author(s)
      Simin Rahighi, Fumiyo Ikeda, Masato Kawasaki, Masato Akutsu, Nobuhiro Suzuki, Ryuichi Kato, Tobias Kensche, Tamami Uejima, Stuart Bloor, David Komander, Felix Randow, Soichi Wakatsuki, Ivan Dikic
    • Journal Title

      Cell 136

      Pages: 1098-1109

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Specific Recognition of Linear Ubiquitin Chains by NEMO Is Important for NF-kappaB Activation2009

    • Author(s)
      Simin Rahighi
    • Journal Title

      Cell 136

      Pages: 1098-1109

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] NEMO selectively binds to ubiquitin chain in NF-κB activation2009

    • Author(s)
      Simin Rahighi, Fumiyo Ikeda, Masato Kawasaki, Masato Akutsu, Nobuhiro Suzuki, Ryuichi Kato, Tobias Kensche, Tamami Uejima, Stuart Bloor, David Komander, Felix Randow, Soichi Wakatsuki, Ivan Dikic
    • Organizer
      第61回日本細胞生物学会大会
    • Place of Presentation
      名古屋国際会議場、名古屋
    • Year and Date
      2009-06-02
    • Related Report
      2008 Final Research Report
  • [Presentation] NEMO (NF-kappaB essntial modulator) selectively binds linear ubiquitin chains in NF-kappaB activation2009

    • Author(s)
      Simin Rahighi, Fumiyo Ikeda, Masato Kawasaki, Masato Akutsu, Nobuhiro Suzuki, Ryuichi Kato, Tobias Kensche, Tamami Uejima, Stuart Bloor, David Komander, Felix Randow, Soichi Wakatsuki, Ivan Dikic
    • Organizer
      第26回PFシンポジウム
    • Place of Presentation
      つくば国際会議場、つくば
    • Year and Date
      2009-03-25
    • Related Report
      2008 Final Research Report
  • [Presentation] NEMO (NF-kappaB essntial modulator) selectively binds linear ubiquitin chains in NF-kappaB activation2009

    • Author(s)
      Simin Rahighi
    • Organizer
      第26回PFシンポジウム
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2009-03-25
    • Related Report
      2008 Annual Research Report
  • [Presentation] Crystallographic study of the ubiquitin-binding zinc finger domain of human polymerase eta2008

    • Author(s)
      Nobuhiro Suzuki, Masato Kawasaki, Ryuichi Kato, Ivan Dikic, Soichi Wakatsuki
    • Organizer
      国際結晶学会IUCr2008
    • Place of Presentation
      グランキューブ大阪国際会議場、大阪
    • Year and Date
      2008-08-26
    • Related Report
      2008 Final Research Report
  • [Presentation] Structure determination of NEMO (NF-kappaB essentialmodulator) UBAN domain2008

    • Author(s)
      Simin Rahighi, Masato Akutsu, Nobuhiro Suzuki, Masato Kawasaki, Ryuichi Kato, Ivan Dikic, Soichi Wakatsuki
    • Organizer
      国際結晶学会 IUCr2008
    • Place of Presentation
      グランキューブ大阪国際会議場、大阪
    • Year and Date
      2008-08-26
    • Related Report
      2008 Final Research Report
  • [Presentation] Structure determination of NEMO(NF-kappaB essential modulator) UBAN domain2008

    • Author(s)
      Simin Rahighi
    • Organizer
      国際結晶学会IUCr2008
    • Place of Presentation
      グランキューブ大阪国際会議場
    • Year and Date
      2008-08-26
    • Related Report
      2008 Annual Research Report
  • [Presentation] Crystallographic study of the ubiquitin-binding zinc finger domain of human polymerase eta2008

    • Author(s)
      鈴木喜大
    • Organizer
      国際結晶学会IUCr2008
    • Place of Presentation
      グランキューブ大阪国際会議場
    • Year and Date
      2008-08-26
    • Related Report
      2008 Annual Research Report
  • [Presentation] ヒトDNAポリメラーゼηのユビキチン結合Znフィンガードメインの結晶構造解析2008

    • Author(s)
      鈴木喜大、川崎政人、加藤龍一、Marzena Bienko、Ivan Dikic、若槻壮市
    • Organizer
      第25回PFシンポジウム
    • Place of Presentation
      KEK、つくば
    • Year and Date
      2008-03-19
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] ヒトDNAポリメラーゼηのユビキチン結合UBZドメインの結晶構造2007

    • Author(s)
      鈴木喜大、川崎政人、加藤龍一、Marzena Bienko、Ivan Dikic、若槻壮市
    • Organizer
      第30回日本分子生物学会年会、第80回日本生化学会大会 合同大会(BMB2007)
    • Place of Presentation
      パシフィコ横浜、横浜
    • Year and Date
      2007-12-11
    • Related Report
      2008 Final Research Report
  • [Presentation] ヒトDNAポリメラーゼηのユビキチン結合UBZドメインの結晶構造2007

    • Author(s)
      鈴木 喜大
    • Organizer
      第30回日本分子生物学会年会、第80回日本生化学会大会合同大会(BMB2007)
    • Place of Presentation
      パシフィコ横浜、横浜
    • Year and Date
      2007-12-11
    • Related Report
      2007 Annual Research Report
  • [Presentation] Crystallographic analysis of the ubiquitin-binding zinc finger domain of human DNA polymerase eta2007

    • Author(s)
      Nobuhiro Suzuki, Masato Kawasaki, Ryuichi Kato, Marzena Bienko, Ivan Dikic, Soichi Wakatsuki
    • Organizer
      2nd International Symposium on Diffraction Structural Biology
    • Place of Presentation
      タワーホール船堀、東京
    • Year and Date
      2007-09-12
    • Related Report
      2008 Final Research Report
  • [Presentation] Crystallographic analysis of the ubiquitin-binding zinc finger domain of human DNA polymerase eta2007

    • Author(s)
      Nobuhiro Suzuki
    • Organizer
      2nd International Symposium on Diffraction Structural Biology
    • Place of Presentation
      Tower Hall Funabori,Tokyo
    • Year and Date
      2007-09-12
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.kek.jp/ja/news/press/2009/NEMO.html

    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.kek.jp/newskek/2009/marapr/NEMO.html

    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.kek.jp/ja/news/press/2009/NEMO.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.kek.jp/newskek/2009/marapr/NEMO.html

    • Related Report
      2008 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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