Study for structure and function of DNA methyltransferase
Project/Area Number |
19570130
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Functional biochemistry
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Research Institution | Osaka University |
Principal Investigator |
SUETAKE Isao Osaka University, 蛋白質研究所, 准教授 (80304054)
|
Project Period (FY) |
2007 – 2008
|
Project Status |
Completed (Fiscal Year 2008)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
Keywords | DNAメチル化 / DNAメチルトランスフェラーゼ |
Research Abstract |
本研究では、ゲノムDNA上のメチル化模様の形成機構を生化学的に理解することを目指し、DNAメチル化酵素(Dnmt)の性質を再構成したヌクレオソームを基質にして詳細に試験管内で解析を行った。その結果、DNA上に新規なメチル化模様を書き込むタイプのDnmt3aは、ヌクレオソームの高次構造に依存したメチル化活性を示すという新たな知見が得られた
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Report
(3 results)
Research Products
(19 results)
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[Journal Article] Abnormal DNA Methyltransferase Expression in Mouse Germline Stem Cells Results in Spermatogenic Defects2009
Author(s)
Takashima S, Takehashi M, Lee J, Chuma S, Okano M, Hata K, Suetake I, Nakatsuji N, Miyoshi H, Tajima S, Tanaka Y, Toyokuni S, Sasaki H, Kanatsu-Shinohara M, Shinohara T.
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Journal Title
Biol Reprod [Epub ahead of print]
Related Report
Peer Reviewed
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