The study of functions of diacylglycerol kinase δ in signal transduction of epidermal growth factor receptor

• Project/Area Number: 19570136
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Sapporo Medical University
• Principal Investigator: IMAI Shin-ichi Sapporo Medical University, 医学部, 講師 (20213209)
• Co-Investigator(Kenkyū-buntansha): SAKANE Fumio 札幌医科大学, 医学部, 准教授 (10183815)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ジアシルグリセロールキナーゼ / ジアシルグリセロール / ホスファチジン酸 / RACK1 / 上皮増殖因子

Research Abstract

上皮増殖因子受容体のシグナル伝達において、脂質リン酸化酵素ジアシルグリセロールキナーゼ(DGK)δがどのような役割を果たしているかを解明するために、本研究ではDGKδとアダプター蛋白質RACK1(receptor for activated C kinase1)の結合の解析を行った。その結果、RACK1はII型のDGKアイソザイムに選択的に結合し、I型とは結合しないこと、また複数のDGKアイソザイムとRACK1の結合が細胞外刺激によりダイナミックに調節されていることが明らかになった。

Research Products

• [Journal Article] Diacylglycerol kinase δ associates with receptor for activated C kinase 1, RACK1 (2009)
  • Author(s): Imai S, Yasuda S, Kai M, Kanoh H, Sakane F
  • Journal Title: Biochim. Biophys. Acta 1791 Pages: 246-253
  • Peer Reviewed
• [Journal Article] Diacylglycerol kinaseδassociates with receptor for activated C kinase I, RACKl. (2009)
  • Author(s): Imai, S.
  • Journal Title: Biochim. Biophys. Acta 1791 Pages: 246-253
  • Peer Reviewed
• [Journal Article] Diacylglycerol kinases as emerging potential drug targets for a variety of diseases (2008)
  • Author(s): Sakane F, Imai S, Kai M, Yasuda S, Kanoh H
  • Journal Title: Curr. Drug Targets 9 Pages: 626-640
  • Peer Reviewed
• [Journal Article] Regulation of enzyme localization by polymerization : polymer formation by the SAM domain of diacylglycerol kinase δ1 (2008)
  • Author(s): Harada BT, Knight MJ, Imai S, Qiao F, Ramachander R, Sawaya MR, Gingery M, Sakane F, Bowie JU
  • Journal Title: Structure 16 Pages: 380-387
  • Peer Reviewed
• [Journal Article] Phorbol ester and hydrogen peroxide synergistically induce the interaction of diacylglycerol kinase γ with the Src homology 2 and C1 domains of β2-chimaerin (2008)
  • Author(s): Yasuda S, Kai M, Imai S, Kanoh H, Sakane F
  • Journal Title: Biochem. J 409 Pages: 95-106
  • Peer Reviewed
• [Journal Article] Rab7 regulates maturation of the melanosomal matrix protein gp100/Pmel17/Silv (2008)
  • Author(s): Kawakami A, Sakane F, Imai S, Yasuda S, Kai M, Kanoh H, Jin H-Y, Hirosaki K, Yamashita T, Fisher D. E, Jimbow K
  • Journal Title: J. Invest. Dermatol 128 Pages: 143-150
  • Peer Reviewed
• [Journal Article] Diacylglycerol kinases as emerging potential drug targets fora variety of diseases. (2008)
  • Author(s): Sakane, F.
  • Journal Title: Curr. Drug Targets 9 Pages: 626-640
  • Peer Reviewed
• [Journal Article] Regulation of enzyme localization by polymerization: Polymer formation by the SAM domain of diacylglycerol kinase δ1. (2008)
  • Author(s): Harada, B. T.
  • Journal Title: Structure 16 Pages: 380-387
  • Peer Reviewed
• [Journal Article] Rab7 regulates maturation of the melanosomal matrix protein gp100/Pmel17/Silv. (2008)
  • Author(s): Kawakami, A.
  • Journal Title: J. Invest. Dermatol. 128 Pages: 143-150
  • Peer Reviewed
• [Journal Article] Phorbol ester and hydrogen peroxide synergistically induce the interaction of diacylglycerolkinase γ with the Src homology 2 and C1 domains of β2-chimaerin. (2008)
  • Author(s): Yasuda, S.
  • Journal Title: Biochem. J. 409 Pages: 95-106
  • Peer Reviewed
• [Journal Article] Tyrosine phosphorylation of β2-chimaerin by Src-family kinase negatively regulates its Rac-specific GAP activity. Biochim (2007)
  • Author(s): Kai M, Yasuda S, Imai S, Kanoh H, Sakane F
  • Journal Title: Biophys. Acta 1773 Pages: 1407-1415
  • Peer Reviewed
• [Journal Article] Diacylglycerol kinases : why so many of them? (2007)
  • Author(s): Sakane F, Imai S, Kai M, Yasuda S, Kanoh H
  • Journal Title: Biochim. Biophys. Acta 1771 Pages: 793-806
  • Peer Reviewed
• [Journal Article] Diacylglycerol kinase α suppresses tumor necrosis factor-α-induced apoptosis of human melanoma cells through NF-κB activation (2007)
  • Author(s): Yanagisawa K, Yasuda S, Kai M, Imai S, Yamada K, Yamashita T, Jimbow K, Kanoh H, Sakane F
  • Journal Title: Biochim. Biophys. Acta 1771 Pages: 462-474
  • Peer Reviewed
• [Journal Article] Diacylglycerol kinase γ interacts with and activates β2-chimaerin, a Rac-specific GAP, in response to epidermal growth facto (2007)
  • Author(s): Yasuda S, Kai M, Imai S, Kanoh H, Sakane F
  • Journal Title: FEBS Lett 581 Pages: 551-557
  • Peer Reviewed
• [Journal Article] Tyrosine phosphorylation of β2-chimaerin by Src-family kinase negatively regulates its Rac-specific GAP activity. (2007)
  • Author(s): Kai, M.
  • Journal Title: Biochim. Biophys. Acta -Mol. Cell Res. 1773 Pages: 1407-1415
  • Peer Reviewed
• [Journal Article] Diacylglycerol kinases: Why so many of them? (2007)
  • Author(s): Sakane, F.
  • Journal Title: Biochim. Biophys. Acta -Mol. Cell Biol. Lipids. 1771 Pages: 793-806
  • Peer Reviewed
• [Journal Article] Diacylglycerol klnase α suppresses tumor necrosis factor-α-induced apoptosis of human melanoma cells through NF-κB activation. (2007)
  • Author(s): Yanagisawa, K.
  • Journal Title: Biochim. Biophys. Acta -Mol. Cell Biol. Lipids. 1771 Pages: 462-474
  • Peer Reviewed
• [Journal Article] Diacylglycerol kinase γ interacts with and activates β2-chimaerin, a Rac-specific GAP, in response to epidermal growth factor. (2007)
  • Author(s): Yasuda, S.
  • Journal Title: FEBS Lett. 581 Pages: 551-557
  • Peer Reviewed
• [Presentation] ジアシルグリセロールキナーゼδはRACK1(receptor for activated Ckinase 1)と会合する (2008)
  • Author(s): 今井伸一
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] ジアシルグリセロールキナーゼδはRACKl(receptor for activated C kinase l)と会合する (2008)
  • Author(s): 今井伸一
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] ジアシルグリセロールキナーゼδはRACK1(receptor for activated Ckinase 1)と結合する (2008)
  • Author(s): 今井伸一
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] ジアシルグリセロールキナーゼδはRACKl(receptor foractivated C kinase l)と結合する (2008)
  • Author(s): 今井伸一
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] ジアシルグリセロールキナーゼδとRACK1は互いの複数のドメインを介して結合している (2007)
  • Author(s): 今井伸一
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12