Analysis of the movements of single axonemal dyneins on microtubules visualized using quantum dots

• Project/Area Number: 19570150
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Kyoto University
• Principal Investigator: YAGI Toshiki Kyoto University, 大学院・理学研究科, 准教授 (40292833)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ダイニン / 微小管 / 鞭毛 / 繊毛 / ATP / 蛍光標識 / 一分子 / 鞭毛・繊毛 / アビジン・ビオチン / 蛍光標織 / 1分子

Research Abstract

本研究では、鞭毛運動におけるモーター蛋白質ダイニンの機能を理解するために、新に開発した鞭毛蛋白質の特異的標識法を利用して、ダイニン1分子の運動の様子と力発生過程を調べた。その結果、鞭毛ダイニンの効率的な力発生と運動には多分子が必要であることが示唆された。また、精製した鞭毛ダイニンのモーター活性を生体内に近い条件で調べる方法も新たに開発した。その方法により、3種類のモーター蛋白質が分子内に共存するタイプの鞭毛ダイニンでは、それぞれが互いに制御しあい協調して機能していることを見出した。

Research Products

• [Journal Article] The dynein stalk contains an antiparallel coiled coil with region-specific stability (2009)
  • Author(s): Hook P, Yagi T, Ghosh-Roy A, Williams J, Vallee RB
  • Journal Title: Biochemistry
• [Journal Article] Systematic comparison of in vitro motile properties between chlamydomonas wild-type and mutant outer arm dyneins each lacking one of the three heavy chains (2009)
  • Author(s): Furuta A, Yagi T, Yanagisawa HA, Higuchi H, Kamiya R
  • Journal Title: J Biol Chem 284(9) Pages: 5927-35
• [Journal Article] Identification of novel dyneins that localize exclusively to the proximal portion of Chlamydomonas (2009)
  • Author(s): Yagi T, Uematsu K., Liu Z., Kamiya R.
  • Journal Title: Journal of Cell Science (in press)
  • Peer Reviewed
• [Journal Article] The dynein stalk contains an antiparallel coiled coil with region-specific stability. (2009)
  • Author(s): Hook P, Yagi T, Ghosh-Roy A, Williams J, Vallee RB.
  • Journal Title: Biochemistry (in press)
  • Peer Reviewed
• [Journal Article] A Novel Ankyrin-Repeat Protein Interacts with the Regulatory Proteins of Inner Arm Dynein f (I1) of Chlamydomonas reinhardtii (2009)
  • Author(s): Ikeda K., Yamamoto R., Wirschell M., Yagi T., Bower R., M. E. Porter, W. S. Sale
  • Journal Title: Cell Motility and the Cytoskeleton (in press)
  • Peer Reviewed
• [Journal Article] Systematic comparison of in vitro motie properties between chiamydomonas wild-type and mutant outer arm dyneins each lacking one of the three heavyr, chains (2009)
  • Author(s): Furuta A, Yagi T, Yanagisawa HA, Higuchi H, Kamiya R.
  • Journal Title: Journal of Biological Chemistry 284 Pages: 5927-5935
  • Peer Reviewed
• [Journal Article] A Novel Ankyrin-Repeat Protein Interacts with the Regulatory Proteins of Inner Arm Dynein f(I1)of Chlamydomonas reinhardtii (2008)
  • Author(s): Ikeda K., Yamamoto R., Wirschell M., Yagi T., Bower R., M. E. Porter, W. S. Sale and R. Kamiya
  • Journal Title: Cell Motil. Cytoskeleton
• [Journal Article] Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins (2008)
  • Author(s): Omran H, Kobayashi D, Olbrich H, (他著者23人中、14番目)
  • Journal Title: Nature 456(7222) Pages: 611-6
• [Journal Article] Partially functional outer arm dynein in a novel Chlamydomonas mutant expressing a truncated γ heavy chain (2008)
  • Author(s): Liu Z., Takazaki H., Nakazawa Y., Sakato M., Yagi T., Yasunaga T., King S. M., and Kamiya R
  • Journal Title: Eukaryotic Cell 7(7) Pages: 1136-1145
  • NAID: 120006345414
• [Journal Article] Novel 44-kilodalton subunit of axonemal Dynein conserved from chlamydomonas to mammals (2008)
  • Author(s): Yamamoto R, Yanagisawa HA, Yagi T, Kamiya R
  • Journal Title: Eukaryot Cell 7(1) Pages: 154-61
• [Journal Article] Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins. (2008)
  • Author(s): Omran H, Kobayashi D, Olbrinch H, Tsukahara T, Loges NT, Hagiwara H, Zhang Q, Leblond G
  • Journal Title: Nature 456 Pages: 611-616
  • Peer Reviewed
• [Journal Article] Partially functional outer arm dynein in a novel Chlamydomonas mutant expressing a truncated γ heavy chain. (2008)
  • Author(s): Liu Z., Takazaki H., Nakazawa Y., Sakato M., Yagi T., Yasunaga T., King S. M.
  • Journal Title: Eukaryotic Cell 7 Pages: 1136-1145
  • NAID: 120006345414
  • Peer Reviewed
• [Journal Article] Novel 44-kilodalton subunit of axonemal Dynein conserved from chlamydomonas to mammals. (2008)
  • Author(s): Yamamoto R, Yanagisawa HA, Yagi T, Kamiya R.
  • Journal Title: Eukaryotic Cell 7 Pages: 154-161
  • Peer Reviewed
• [Journal Article] Identification of novel dyneins that localize exclusively to the proximal portion of Chlamydomonas flagella
  • Author(s): Yagi T, Uematsu K., Liu Z., and Kamiya R
  • Journal Title: J. Cell Sci 122 Pages: 1306-1314
• [Presentation] クラミドモナス鞭毛の根元に局在する新規内腕ダイニンの同定 (2008)
  • Author(s): 八木俊樹、植松圭吾、劉中美、神谷律
  • Organizer: 日本生物物理学会第46回年会
• [Presentation] Identificaiton of three novel dyneins localized exclusively to the proximal region of Chlamydomonas flagella (2008)
  • Author(s): Yagi T, Uematsu K., Liu Z., and Kamiya R
  • Organizer: 2nd International Symposium on Bio-nanosystems
• [Presentation] Identificaiton of three minor inner-arm dynein heavy chains in Chlamydomonas flagella (2008)
  • Author(s): Yagi T, and Kamiya R
  • Organizer: EMBO Workshop on the Cell and Molecular Biology of Chlamydomonas
• [Presentation] Identificaiton of three minoir inner-arm dynein heavy chains in Chlamydomonas flagella (2008)
  • Author(s): Yagi T, Uematsu K., Liu Z., Kamiya R.
  • Organizer: EMBO Workshop on the Cell and Molecular Biology of Chlamydomonas
  • Place of Presentation: Hyerres-les-Palmiers, Var, France
• [Presentation] Identificaiton of three novel dyneins localized exclusively to the proximal region of Chlamydomonas (2008)
  • Author(s): Yagi T, Uematsu K., Liu Z., Kamiya R.
  • Organizer: 2nd International Symposium on Bio-nanosystems
  • Place of Presentation: 東京大学小柴ホール
• [Presentation] クラミドモナス鞭毛の根元に局在する新規内腕タイニンの同定 (2008)
  • Author(s): 八木俊樹, 植松圭吾, 劉中美, 神谷律
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] クラミドモナス鞭毛における3種のマイナーダイニン重鎖の同定 (2007)
  • Author(s): 八木俊樹、神谷律
  • Organizer: 日本生物物理学会、第45回年会
• [Presentation] Identificaiton of three minor inner-arm dynein heavy chains in Chlamydomonas flagella (2007)
  • Author(s): Yagi T, and Kamiya R
  • Organizer: FASEB Summer Research Conferences, Biology of Cilia and Falgella
• [Presentation] Identification of three minor inner-arm dynein heavy chains in Chlamydomonas flagella (2007)
  • Author(s): Toshiki Yagi, Keigo Uematsu and Ritsu Kamiya
  • Organizer: FASEB meeting
  • Place of Presentation: Vermont Academy, Vermont, USA