Study of the dynamic structure of the troponin-tropomyosin complex

• Project/Area Number: 19570152
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: University of Fukui
• Principal Investigator: 三木 正雄 University of Fukui, 工学研究科, 教授 (30242580)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: タンパク質 / 核酸の構造 / 動態 / 機能 / 生物物理 / 分子スイッチ / 蛍光測定 / 制御メカニズム

Research Abstract

トロポミオシンやトロポニン複合体(TnC, TnI, TnT)の遺伝子操作により、おのおの、シングルシステイン残基をもつ変異体を作り出して、そのシステイン残基それぞれに蛍光色素をラベルして、蛍光測定を行うことにより、トロポミオシンートロポニン、トロポミオシンーアクチン、トロポニンートロポミオシンの相互作用について明らかにした。

Research Products

• [Journal Article] Fluorescence Resonance Energy Transfer between Residues on Troponin and Tropomyosin in the Reconstituted Thin Filament : Modeling the Troponin-Tropomyosin Complex Kimura-Sakiyama (2008)
  • Author(s): C., Ueno, Y., Wakabayashi, K., and Miki, M.
  • Journal Title: J.Mol.Biol 376 Pages: 80-91
  • Peer Reviewed
• [Journal Article] Fluorescence resonance energy transfer between residues on troponin and tropomyosin in the reconstituted thin filament : Modeling the tropon in-tropomyosin complex (2008)
  • Author(s): Kimura-Sakiyama, C., Ueno, U., Wakabayashi, K., Miki, M.
  • Journal Title: J. Mol. Biol 376 Pages: 80-91
  • Peer Reviewed
• [Journal Article] Fluorescence resonance energy transfer between residues on troponin and tropomyosin in the reconstituted thin filament: Modeling the troponin-tropomyosin complex (2008)
  • Author(s): Kimura-Sakiyama, C., Ueno, U., Wakabayashi, K. and Miki, M.
  • Journal Title: J. Mol. Biol. 376 Pages: 80-91
  • Peer Reviewed
• [Journal Article] Conformational Changes in Reconstituted Skeletal Muscle Thin Filaments Observed By Fluorescence Spectroscopy (2007)
  • Author(s): Miki, M
  • Journal Title: Adv.Exp.Med. Biol 592 Pages: 111-123
  • NAID: 120000808496
  • Peer Reviewed
• [Journal Article] Fluorescence emission from PAMAM and PPI dendrimers
  • Author(s): Wang, D., Imae, T., and Miki, M
  • Journal Title: Journal of Colloid and Interface Science
• [Presentation] 再構成細いフィラメント上でのアクチンートロポミオシン残基間の蛍光エネルギー移動測定 : アクチ ンートロポミオシン複合体モデル (2009)
  • Author(s): 三木正雄, 斎藤隆広、飛田英孝、上野豊、木邑智恵子
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 東京大学数理科学研究科
  • Year and Date: 2009-01-10
• [Presentation] 再構成細いフィラメント上でのアクチンートロポミオシン残基間の蛍光エネルギー移動測定 : アクチンートロポミオシン複合体モデル (2009)
  • Author(s): 三木正雄, 斎藤隆広, 飛田英孝, 上野豊, 木邑智恵子
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 東京大学数理科学研究科
  • Year and Date: 2009-01-10
• [Presentation] FRET between Residues on Tropomyosin and Actin in the Reconstituted Thin Filament : Modeling the Actin-Tropomyosin Complex (2008)
  • Author(s): T. Saitoh, Y. Ueno, C. Kimura, and M. Miki
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-12-04
• [Presentation] 筋肉細いフィラメント上のトロポミオシンートロポニンの構造変化 (2008)
  • Author(s): Miki, M
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 仙台市戦災復興記念館 記念ホール
  • Year and Date: 2008-01-07
• [Presentation] Study of the dynamic structure of the tropomyosin molecule in muscle thin filaments by fluorescence quenching measurements (2007)
  • Author(s): Takahiro Saitoh, Yousuke Matsuda, and Masao Miki
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-22
• [Presentation] Study of the dynamic structure of the tropomyosin molecule in muscle thin filaments by fluorescence quenching measurements. (2007)
  • Author(s): Takahiro Saitoh, Yousuke Matsuda, and Masao Miki
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-22
• [Presentation] Application of fluorescence resonance energy transfer to muscle proteins (2007)
  • Author(s): Miki, M.
  • Organizer: The 7th KIAS-Soongsil Conference on Protein Structure and Function
  • Place of Presentation: Soongsil University, Seoul, Korea
  • Year and Date: 2007-10-06
• [Presentation] Applications of fluorescence resonance energy transfer to muscle proteins.
  • Author(s): Miki, M
  • Organizer: The 7^<th> KIAS-Soogsil Conference on protein structure and Function
  • Place of Presentation: Soongsil University, Seoul, Korea(招待講演)
• [Book] 生物物理学ハンドブック(石渡信一他編集)
  • Author(s): 三木正雄
  • Publisher: 朝倉書店