| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Neuroserpin is a neuronal protein predominantly expressed in the brain, and its mutants readily form aggregates that cause dementia. Several compounds that prevent aggregation of neuroserpin were found, and crystals of human neuroserpin were successfully obtained using them. The crystal structure was determined at 2.1Å, and interactions between the protein and the compound were analyzed. Furthermore, the compounds were effective in stabilizing a refolding intermediate of neuroserpin, which is promising for a target molecule in therapeutic drug design. Using the compounds, the intermediate was purified and characterized.
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