Analysis of E2F-mediated transcriptional mechanism, which specifically senses oncogenic changes

• Project/Area Number: 19570163
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: OHTANI Kiyoshi Tokyo Medical and Dental University, 疾患遺伝子実験センター, 講師 (30201974)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 転写 / 癌化 / E2F / pRB / p53 / ARF / p27KiP1 / RB / 癌性変化 / 転写制御 / p27Kip1 / TP73 / アポトーシス / 癌抑制遺伝子 / p14^<ARF> / p27^<Kipl> / DP

Research Abstract

代表的な癌抑制遺伝子産物pRBの標的である転写因子E2Fの癌化抑制に関わる制御機構を解析した。E2Fは、pRBの機能が失われると、増殖刺激では活性化されないサイクリン依存性キナーゼ抑制因子p27Kip1など癌化抑制に関わる遺伝子の発現を誘導することを明らかにした。癌化抑制に関わる制御機構は、結合パートナーであるDPに対する依存性が極めて低く、細胞増殖制御に関わる機構とは異なることを明らかにした。

Research Products

• [Journal Article] 染色体DNAの複製と安定性を司るRB/E2F (2009)
  • Author(s): 左谷渣, 大園瑛子
  • Journal Title: 蛋白質・核酸・酵素 54 Pages: 327-333
  • Peer Reviewed
• [Journal Article] E2F-like elements in p27K'Pl promoter specifically sense deregulated E2F activity (2009)
  • Author(s): Ozono, E., Komori, H., Iwanaga, R., Ikeda, MA., Iseki, S., Ohtani, K
  • Journal Title: Genes Cells 14 Pages: 89-99
  • Peer Reviewed
• [Journal Article] E2F-like elements in p27^<kip1> promoter specifically sense deregulated E2F activity. (2009)
  • Author(s): Ozono, E., Komori, H., Iwanaga, R., Ikeda, MA., Iseki, S., Ohtani, K.
  • Journal Title: Genes to Cells 14 Pages: 89-99
  • Peer Reviewed
• [Journal Article] 染色体DNA複製と安定性を司るRB/E2F (2009)
  • Author(s): 大谷清, 大園瑛子
  • Journal Title: 蛋白質・核酸・酵素 54 Pages: 327-333
  • Peer Reviewed
• [Journal Article] Activation of the cyclin D2 and cdk6 genes through NF-KB is critical for cell cycle progression induced by HTLV-I Tax (2008)
  • Author(s): Iwanaga, R., Ozono, E., Fujisawa, J., Ikeda, MA., Okamura, N., Huang, Y., Ohtani, K
  • Journal Title: Oncogene 27 Pages: 5635-5642
  • Peer Reviewed
• [Journal Article] A role of human T-cell leukemia virus type I Tax in expression of the human telomerase reverse transcriptase gene in human T cells (2008)
  • Author(s): Hara, T., Matsumura-Arioka, Y., Ohtani, K., Nakamura, M
  • Journal Title: Cancer Science 29 Pages: 1155-1163
  • Peer Reviewed
• [Journal Article] A role of human T-cell leukemia virus type I Tax in expression of the human telomerase reverse transcriptase gene in human T cells. (2008)
  • Author(s): Hara, T., Matsumura-Arioka, Y., Ohtani, K., Nakamura, M.
  • Journal Title: Cancer Science 99 Pages: 1155-1163
  • Peer Reviewed
• [Journal Article] Activation of the cyclin D2 and cdk6 genes through NF-kB is critical for cell cycle progression induced by HTLV-I Tax. (2008)
  • Author(s): Iwanaga, R., Ozono, E., Fujisawa, J., Ikeda, MA., Okamura, N., Huang, Y., Ohtani, K.
  • Journal Title: Oncogene 27 Pages: 5635-5642
  • Peer Reviewed
• [Journal Article] HTLV-1 Tax-induced NF K B activation is independent of Lys-63-linked-type polyubiquitination (2007)
  • Author(s): Gohda, J., Irisawa, M., Tanaka, Y., Sato, S., Ohtani, K., Fujisawa, J., Inoue, J
  • Journal Title: Biochem Biophys Res Commun 357 Pages: 225-230
  • Peer Reviewed
• [Journal Article] HTLV-I Tax-induced NF-κB activation is independent of Lys-63-linked-type polyubiquitination. (2007)
  • Author(s): Gohda J, Irisawa M, Tanaka Y, Sato S, Ohtani K, Fujisawa J and Inoue J
  • Journal Title: Biochem. Biophys. Res. Commun. 357 Pages: 225-230
  • Peer Reviewed
• [Presentation] 転写因子E2Fによる癌性変化と増殖刺激の識別 (2008)
  • Author(s): 大園瑛子, 池田正明, 井関幸子, 大谷清
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] The Cdk inhibitor p27Kip1 gene senses pRB dysfunction through E2F (2008)
  • Author(s): Eiko Ozono, Masa-aki Ikeda, Kiyoshi Ohtani
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] E2FのヘテロダイマーパートナーDP1はE2Fが誘導するアポトーシスとp14ARF遺伝子発現には必要ない (2008)
  • Author(s): 小森英之, 中村正孝, 大谷清
  • Organizer: 第30回日本分子生物学会年回
  • Place of Presentation: 横浜
• [Presentation] p27Kir1 promoter specifically senses deregulated E2F activity (2008)
  • Author(s): Ozono E, Komori H, Ikeda M, Ohtani K
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] The Cdk inhibitor p27Kip1 gene senses pRB dysfunction through E2F. (2008)
  • Author(s): Eiko Ozono, Masa-aki Ikeda, Kiyoshi Ohtani
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] p27^<Kip1> promoter specifically senses deregulated E2F activity. (2007)
  • Author(s): Ozono E, Komori H, Ikeda M and Ohtani K
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] E2FのヘテロダイマーパートナーDP1はE2Fが誘導するアポトーシスとp14^ARF遺伝子発現には必要ない. (2007)
  • Author(s): 小森英之、中村正孝、大谷清
  • Organizer: 第30回日本分子生物学会年回
  • Place of Presentation: 横浜
• [Book] Yoshida, K. ed., Research Signpost (2009)
  • Author(s): Ohtani, K., Komori, H., Ozono, E., Ikeda, M-A., Iwanaga R
  • Publisher: Distinct Transcriptional Regulation by E2F in Cell Growth and Tumor Suppression. in "Control of cellular physiology by the transcription factor E2F"
• [Book] Distinct transcriptional regulation by E2F in cell growth and tumor suppression. in "Control of cellular physiology by the transcription factor E2F" (2008)
  • Author(s): Ohtani, K., Komori, H., Ozono, E., Ikeda, M-A., Iwanaga R.
  • Total Pages: 15
  • Publisher: Research Signpost