Recognition of diverse nucleotide sequences by heat shock transcription factors of various organisms

• Project/Area Number: 19570164
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Kanazawa University
• Principal Investigator: SAKURAI Hiroshi Kanazawa University, 保健学系, 準教授 (00225848)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: ストレス応答 / 熱ショック転写因子 / 培養細胞 / 酵母菌

Research Abstract

ストレス対応反応において重要な役割を担う熱ショックタンパク質群の遺伝子発現は、プロモーター領域にあるHSE配列とこれに結合する熱ショック転写因子(HSF)により制御されている。本研究では、酵母, シロイヌナズナ, 線虫, ショウジョウバエ, ゼブラフィッシュ, ヒトのHSFのHSE配列特異性について検討して。生化学的、および、酵母菌・HeLa細胞での発現実験より、これらのHSFは多様なHSE配列を認識することが明らかになった。HSE配列の多様性は、ストレスや遺伝子特異的な転写に関すると考えられる。

Research Products

• [Journal Article] Differential recognition of heat shock elements by members of the heat shock transcription factor family (2009)
  • Author(s): Noritaka Yamamoto, Yukiko Takemori, Mayumi Sakurai, Kazuhisa Sugiyama, and Hiroshi Sakurai
  • Journal Title: FEBS Journal 276 Pages: 1962-1974
  • Peer Reviewed
• [Journal Article] Differential recognition of heat shock elements by members of theheat shock transcription factor family. (2009)
  • Author(s): Yamampto, N., et al.
  • Journal Title: FEBS Journal 276 Pages: 1962-1974
  • Peer Reviewed
• [Journal Article] (2008)Regulation of thermotolerance by stress-induced transcription factors in Saccharomyces cerevisiae (2008)
  • Author(s): Noritaka Yamamoto, Yuka Maeda, Aya Ikeda, and Hiroshi Sakurai
  • Journal Title: Eukaryotic Cell 7 Pages: 783-790
  • Peer Reviewed
• [Journal Article] resistance and TOR signaling in Yeast revealed by combined proteomic and genetic analysis (2008)
  • Author(s): Sricharan Bandhakavi, Hongwei Xie, Brennon O'Callaghan, Hiroshi Sakurai, Do-Hyung Kim, Timothy and J. Griffin
  • Journal Title: PLoS One 3
  • Peer Reviewed
• [Journal Article] Regulation of thermotolerance by stress-induced transcription factors in Saccbaromyces cerevisiae. (2008)
  • Author(s): Yamamoto, N., et al.
  • Journal Title: Eukaryotic Cell 7 Pages: 783-790
  • Peer Reviewed
• [Journal Article] HSF1 activation inhibits rapamycin resistance and TOR signaling in Yeast revealed by combined proteomic and genetic analysi (2008)
  • Author(s): Bandhakavi, S., et. al.
  • Journal Title: PLoS ONE 3
  • Peer Reviewed
• [Journal Article] Role of heat shock transcription factor in the Saccharomyces cerevisiae oxidative stress response (2007)
  • Author(s): Ayako Yamamoto, Junko Ueda, Noritaka Yamamoto, Naoya Hashikawa, and Hiroshi Sakurai
  • Journal Title: Eukaryotic Cell 6 Pages: 1373-1379
  • Peer Reviewed
• [Journal Article] Interaction between heat shock transcription factors (HSFs) and divergent binding sequences : binding specificities of yeast HSFs and human HSF1 (2007)
  • Author(s): Hiroshi Sakurai and Yukiko Takemori
  • Journal Title: Journal of Biological Chememistry 282 Pages: 13334-13341
  • Peer Reviewed
• [Journal Article] Different mechanisms are involved in the transcriptional activation by heat shock transcription factor through different typesof heat shock elements (2007)
  • Author(s): Naoya Hashikawa, Noritaka Yanamoto and Hiroshi Sakurai
  • Journal Title: Journal of Biological Chememistry 282 Pages: 10333-10340
  • Peer Reviewed
• [Journal Article] Role of heat shock transcription factor in the Saccharomyces cerevisiae oxidative stress response (2007)
  • Author(s): Yamamoto, A., et. al.
  • Journal Title: Eukaryotic Cell 6 Pages: 1373-1379
  • Peer Reviewed
• [Journal Article] Interaction between heat shock transcription factors(HSFs) and divergent binding sequences: binding specificities of yeast HSFs and human HSF1 (2007)
  • Author(s): Sakurai, H., et. al.
  • Journal Title: Journal of Biological Chemistry 282 Pages: 13334-13341
  • Peer Reviewed
• [Presentation] 熱ショック転写因子ファミリーの異なったDNA塩基配列認識 (2008)
  • Author(s): 山本慶隆, 竹森優喜子, 榎康明, 桜井博
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 酵母熱ショック転写因子(Hsf1)による遺伝子特異的なメディエーターのリクルートメント (2008)
  • Author(s): 橋川直也, 桜井博
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 金属イオン誘導性人工転写因子の設計と構築 (2008)
  • Author(s): 村瀬茂雄, 舟越靖, 山上健, 石野良純, 山本慶隆, 桜井博, 田中俊樹
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 酵母熱ショック転写因子(Hsfl)による遺伝子特異的なメデイェータ-のリクルートメント (2008)
  • Author(s): 橋川直也, 桜井博
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 金属誘導型転写因子タンパク質の設計 (2008)
  • Author(s): 菅原大, 舟越靖, 山本慶隆, 桜井博, 山上健, 石野良純, 田中俊樹
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2008-09-18
• [Presentation] ヒト熱ショック転写因子による結合配列特異的な転写調節 (2007)
  • Author(s): 山本 慶隆 ら
  • Organizer: 第30回日本分子生物学会年会・第80回生化学会大会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Presentation] ヒト熱ショック転写因子による結合配列特異的な転写調節 (2007)
  • Author(s): 山本慶隆, 池田彩, 前田夕佳, 竹森優喜子, 桜井博
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11