Genetic and biochemical analysis of ASF1 in chroomatin function

• Project/Area Number: 19570166
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: University of Miyazaki
• Principal Investigator: TAKAMI Yasunari University of Miyazaki, 医学部, 准教授 (80236356)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 染色体構築 / 機能 / 分配 / クロマチン / ヒストン / 遺伝子及び染色体 / 遺伝情報複製

Research Abstract

ゲノムDNAは、細胞内ではヒストンなどの蛋白質と共に様々な高次クロマチン構造を形成することによって、染色体機能が維持される。ヒストンのシャペロン分子と知られるAsf1は新しく作られたヒストンH3-H4を複製されたDNA上に運ぶ重要な分子である。新規合成ヒストンH4はHat1というアセチル化酵素で高度にアセチル化されている。これら分子と相互作用する因子を共免疫沈降法で探索し、複合体成分の解析を行なった.その結果Hat1もしくはH4のLys-5,12のアセチル化が安定した細胞質ヒストンH3-H4複合体形成に寄与し、H3-H4複合体の形成はHat1とAsf1によって制御されていること明らかになった。

Research Products

• [Journal Article] Histone acetyltransferase-1 regulates integrity of cytosolic histone H3-H4 containing complex (2008)
  • Author(s): Barman H K., Takami Y., Nishijima H., Shibahara K., Sanematsu F., and Nakayama T.
  • Journal Title: Biochem. Biophys. Res. Commun. 373 Pages: 624-630
  • Peer Reviewed
• [Journal Article] Histone acetyltransferase-1 regulates integrity of cytosolic histone H3-H4 containing complex. (2008)
  • Author(s): Barman H. K., Takami Y., Nishijima H., Shibahara K-i., Sanematsu F., Nakayama T.
  • Journal Title: Biochem. Biophys. Res. Commun. 373 Pages: 624-630
  • Peer Reviewed
• [Journal Article] Cleavage and cytoplasmic relocalization of histone deacetylase 3 are important for apoptosis progression (2007)
  • Author(s): Escaffit F., Vaute O., Chevillard-Briet M., Segui B., Takami Y., Nakayama T., and Trouche D.
  • Journal Title: Mol. Cell. Biol. 27 Pages: 554-567
  • Peer Reviewed
• [Journal Article] Histone H1 variant, H1R is involved in DNA damageresponse (2007)
  • Author(s): Hashimoto H., Sonoda E., Takami Y., Kimura H., Nakayama T., Tachibana M., Takeda S., and Shinkai Y.
  • Journal Title: DNA Repair (Amst) 6 Pages: 1584-1595
  • Peer Reviewed
• [Journal Article] HDAC2 controls IgM H- and L-chain gene expressions via EBF1, Pax5, Ikaros, Aiolos and E2A gene expressions (2007)
  • Author(s): Nakayama M., Suzuki H., Yamamoto-Nagamatsu N., Barman H K., Kikuchi H., Takami Y., Toyonaga K., Yamashita K., and Nakayama T.
  • Journal Title: Genes Cells 12 Pages: 359-373
  • Peer Reviewed
• [Journal Article] Collaborative roles of gamma H2AX and the Rad51 paralog Xrcc3 in homologous recombinational repair (2007)
  • Author(s): Sonoda E., Zhao G Y., Kohzaki M., Dhar P K., Kikuchi K., Redon C., Pilch D R., Bonner W M., Nakano A., Watanabe M., Nakayama T., Takeda S., and Takami Y.
  • Journal Title: DNA Repair (Amst) 6 Pages: 280-292
  • Peer Reviewed
• [Journal Article] Essential role of chromatin assembly factor-1-mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cells (2007)
  • Author(s): Takami Y., Ono T., Fukagawa T., Shibahara K., and Nakayama T.
  • Journal Title: Mol. Biol. Cell 18 Pages: 129-141
  • NAID: 120007121873
  • Peer Reviewed
• [Journal Article] Essential role of chromatin assembly factor-1-mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cells. (2007)
  • Author(s): Takami, Y., Ono, T., Fukagawa, T., Shibahara, K., and Nakayama, T.
  • Journal Title: Mol Biol Cell 18 Pages: 129-141
  • NAID: 120007121873
  • Peer Reviewed
• [Journal Article] HDAC2 controls IgM H- and L-chain gene expressions via EBF1, Pax5, Ikaros, Aiolos and E2A gene expressions. (2007)
  • Author(s): Nakayama, M., Suzuki, H., Yamamoto-Nagamatsu, N., Barman, H. K., Kikuchi, H., Takami, Y., Toyonaga, K., Yamashita, K., and Nakayama, T.
  • Journal Title: Genes Cells 12 Pages: 359-373
  • Peer Reviewed
• [Journal Article] Histone H1 variant, H1R is involved in DNA damage response. (2007)
  • Author(s): Hashimoto, H., Sonoda, E., Takami, Y., Kimura, H., Nakayama, T., Tachibana, M., Takeda, S., and Shinkai, Y.
  • Journal Title: DNA Repair (Amst) 6 Pages: 1584-1595
  • Peer Reviewed
• [Presentation] DT40細胞を用いHAT1の機能解析 (2008)
  • Author(s): 高見恭成、Barman H K., 中山建男
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] DT40細胞を用いHAT1の機能解析 (2008)
  • Author(s): 高見恭成、Barman H K., 中山建男
  • Organizer: 日本生化学会九州支部例会
  • Place of Presentation: 福岡
• [Presentation] DT40細胞を用いたASF1の機能解析 (2007)
  • Author(s): 高見恭成、中山建男
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-12
• [Presentation] DT40細胞を用いたASF1の機能解析 (2007)
  • Author(s): 高見恭成、中山建男
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: 神戸