Recombination-independent homologous chromosome pairing in fission yeast

• Project/Area Number: 19570173
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: National Institute of Information and Communications Technology
• Principal Investigator: DING Daqiao National Institute of Information and Communications Technology, 未来ICT研究センターバイオICTグループ, 主任研究員 (50359080)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 相同染色体の対合 / 発生・分化 / 分子生物学 / 遺伝学 / Non-Coding RNA / 染色体 / 減数分裂 / 細胞生物学 / 微生物学

Research Abstract

Pairing of homologous chromosome is an essential step in meiosis that ensures effective homologous recombination and reductional segregation in meiotic division. However, no mechanisms for chromosome specific recognition have ever been found. Here we found a novel double stand break independent pairing site, the sme2 locus, on the chromosome II of S.pombe, which shows extraordinary high and stable pairing activity. The sme2 gene encodes a meiosis specific non-coding RNA ; expression of sme2 non-coding RNA is essential for the pairing activity.

Research Products

• [Journal Article] From meiosis to postmeiotic events: Alignment and recognition of homologous chromosomes in meiosis. (2010)
  • Author(s): Ding DQ, Haraguchi T, Hiraoka Y.
  • Journal Title: FEBS J. 277(3) Pages: 565-570
  • Peer Reviewed
• [Journal Article] From meiosis to postmeiotic events : Alignment and recognition of homologous chromosomes in meiosis. (2010)
  • Author(s): DA-QIAO DING
  • Journal Title: FEBS J 277 Pages: 565-570
  • Peer Reviewed
• [Journal Article] Localization of gene products using a chromosomally tagged GFP-fusion library in the fission yeast Schizosaccharomyces pombe. (2009)
  • Author(s): Hayashi A, Ding DQ, Tsutsumi C, Chikashige Y, Masuda H, Haraguchi T, Hiraoka Y
  • Journal Title: Genes Cells 14 Pages: 217-225
  • Peer Reviewed
• [Journal Article] Localization of gene products using a chromosomally tagged GFP-fusion library in the fission yeast Schizosaccharomyces pombe (2009)
  • Author(s): Aki Hayashi
  • Journal Title: Genes to Cells 14 Pages: 217-225
  • Peer Reviewed
• [Presentation] 減数分裂で相同染色体が相互認識する仕組み (2010)
  • Author(s): 平岡泰
  • Organizer: 第26回染色体ワークショップ
  • Place of Presentation: 静岡県御殿場時之栖・日本
  • Year and Date: 2010-01-21
• [Presentation] 減数分裂で相同染色体が相互認識する仕組み (2010)
  • Author(s): 平岡泰
  • Organizer: 第26回染色体ワークショップ
  • Place of Presentation: 静岡県御殿場市時之栖
  • Year and Date: 2010-01-21
• [Presentation] 分裂酵母相同染色体対合におけるsme2ホットスポットの解析 (2009)
  • Author(s): 丁大橋
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市パシフィコ横浜・日本
  • Year and Date: 2009-12-12
• [Presentation] 分裂酵母相同染色体対合におけるsme2ホットスポットの解析 (2009)
  • Author(s): 丁大橋
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市パシフィコ横浜
  • Year and Date: 2009-12-12
• [Presentation] Movements of chromosomes for finding their homologous partners in meiosis (2009)
  • Author(s): 平岡泰
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市パシフィコ横浜・日本
  • Year and Date: 2009-12-10
• [Presentation] Movements of chromosomes for finding their homologous partners in meiosis (2009)
  • Author(s): 平岡泰
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] A non-coding RNA mediat ed homologous chromosome pairing si te in fission yeast (2009)
  • Author(s): 丁大橋
  • Organizer: The 5t h Interna tional Fission Yeast Meeting POMBE 2009
  • Place of Presentation: 東京都国立オリンピック記念青少年総合センター・日本
  • Year and Date: 2009-10-30
• [Presentation] Large-scale localization o f protein components of nuclear structures in fission yeast (2009)
  • Author(s): 平岡泰
  • Organizer: The 5t h In ternational Fission Yeast Meeting POMBE 2009
  • Place of Presentation: 東京都国立オリンピック記念青少年総合センター・日本
  • Year and Date: 2009-10-30
• [Presentation] Large-scale localization of protein components of nuclear structures in fission yeast (2009)
  • Author(s): 平岡泰
  • Organizer: The 5^<th> International Fission Yeast Meeting POMBE 2009
  • Place of Presentation: 東京都国立オリンピック記念青少年総合センター
  • Year and Date: 2009-10-30
• [Presentation] A non-coding RNA mediated homologous chromosome pairing site in fission yeast (2009)
  • Author(s): 丁大橋
  • Organizer: The 5^<th> International Fission Yeast Meeting POMBE 2009
  • Place of Presentation: 東京都国立オリンピック記念青少年総合センター
  • Year and Date: 2009-10-26
• [Presentation] 分裂酵母相同染色体対合におけるsme2ホットスポットの解析 (2009)
  • Author(s): 丁大橋
  • Organizer: 第26回染色体ワークショップ
  • Place of Presentation: 兵庫県姫路市ウェルサンピア姫路ゆめさき・日本
  • Year and Date: 2009-01-27
• [Presentation] 分裂酵母相同染色体対合におけるsme2ホットスポットの解析 (2009)
  • Author(s): 丁大橋
  • Organizer: 第26回染色体ワークショップ
  • Place of Presentation: 姫路
  • Year and Date: 2009-01-27
• [Presentation] A recombination-independent hot spot of homologous chromosome pairing at the sme2 locus in fission yeast (2008)
  • Author(s): 丁大橋
  • Organizer: The American Society for Cell Biology 48th Annual Meeting
  • Place of Presentation: The Moscone Center San Francisco, USA
  • Year and Date: 2008-12-15
• [Presentation] A recombination-independent hot spot of homologous chromosome pairing at the sme2 locus in fission yeast (2008)
  • Author(s): Da-Qiao Ding
  • Organizer: The American Society for Cell Biology 48th Annual Meeting
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2008-12-15
• [Presentation] Chromatin and nuclear structures in fission yeast (2008)
  • Author(s): 平岡泰
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 兵庫県神戸市神戸国際会議場・日本
  • Year and Date: 2008-12-10
• [Presentation] Chromatin and nuclear structures in fission yeast (2008)
  • Author(s): Yasushi Hiraoka
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] Contribution of a non-coding RNA in formation of a double-strand break independent pairing hotspot in meiosis of S. pombe (2008)
  • Author(s): 丁大橋
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 兵庫県神戸市神戸国際会議場・日本
  • Year and Date: 2008-12-09
• [Presentation] Contribution of a non-coding RNA in formation of a double-strand break independent pairing hotspot in meiosis of S. pombe (2008)
  • Author(s): 丁大橋
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] A recombination-independen t hot spot of homologous chromosome pairing at the sme2 locus in fission yeast (2008)
  • Author(s): 丁大橋
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 神奈川県横浜市パシフィコ横浜・日本
  • Year and Date: 2008-06-29
• [Presentation] A recombination-independent hot spot of homologous chromosome pairing at the sme2 locus in fission yeast (2008)
  • Author(s): Da-Qiao Ding
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-29
• [Presentation] Recognition and pairing of homologous chromosome in fission yeast (2007)
  • Author(s): 丁大橋
  • Organizer: EMBO Meiosis Meeting
  • Place of Presentation: 神奈川県湘南国際村センター・日本
  • Year and Date: 2007-09-14
• [Presentation] Recognition and pairing of homologous chromosome in fission yeast (2007)
  • Author(s): Da-Qiao Ding
  • Organizer: EMBO Meiosis Meeting
  • Place of Presentation: Kanagawa, Japan
  • Year and Date: 2007-09-14
• [Presentation] Recognition and pairing of homologous chromosome in fission yeast (2007)
  • Author(s): Da-Qiao Ding
  • Organizer: The Fourth Intemational Fission Yeast Meeting
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2007-07-11
• [Presentation] Recognition and pairing of homologous chromosome in fission yeast (2007)
  • Author(s): 丁大橋
  • Organizer: The Fourth International Fission Yeast Meeting
  • Place of Presentation: Copenhagen大学・Copenhagen Denmark
  • Year and Date: 2007-06-11
• [Presentation] Recombination-independent homologous chromosome pairing in meiosi s of fission yeast (2007)
  • Author(s): 丁大橋
  • Organizer: 第59回日本細胞生物学会大会
  • Place of Presentation: 福岡県福岡市福岡国際会議場・日本
  • Year and Date: 2007-05-28
• [Presentation] Analysis of nuclear organization by making a new GFP library in fission yeast (2007)
  • Author(s): 林亜紀
  • Organizer: 第59回日本細胞生物学会大会
  • Place of Presentation: 福岡県福岡市福岡国際会議場・日本
  • Year and Date: 2007-05-28
• [Presentation] Recombination-independent homologous chromosome pairing in meiosis of fission yeast (2007)
  • Author(s): 丁 大橋
  • Organizer: 第59回日本細胞生物学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2007-05-28
• [Presentation] Analysis of nuclear organization by making a new GFP library in fission yeast (2007)
  • Author(s): 林 亜紀
  • Organizer: 第59回日本細胞生物学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2007-05-28
• [Book] Genome Dynamics & Stability (2007)
  • Author(s): 丁大橋
  • Publisher: Springer
• [Book] Genome Dynamics & Stability (2007)
  • Author(s): Da-Qiao Ding
  • Publisher: Springer(Online first)
• [Remarks]
  • URL: http://www2.nict.go.jp/w/w103/w131103/CellMagic/index.html
• [Remarks]
  • URL: http://www-karc.nict.go.jp/w131103/CellMagic/index.html
• [Remarks]
  • URL: http://www-karc.nict.go.jp/w131103/CellMagic/index.html
• [Remarks]
  • URL: http://www-karc.nict.go.jp/w131103/CellMagic/index.html