Functional analysis of Rab proteins involved in endocytic transport in multicellular organism.

• Project/Area Number: 19570186
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: ANDO Keiko Tokyo Women's Medical University, 総合研究機構脳科学融合研究センター, 特任准教授 (40221741)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 小胞輸送 / 線虫 / エンドサイトーシス / rab / Sec1 / Munc18 / syntaxin / Rabファミリー / 低分子量G蛋白質 / Munc18ファミリー

Research Abstract

A small G protein, Rab, is a major protein family which controls membrane traffic in eucaryotes. There are more than 60 members of Rab in the human genome, however, the biological functions of these proteins are not well understood. To clarify the physiological roles of Rab proteins in the endocytic transport, we isolated deletion mutants of genes encoding Rabs and their cooperating proteins in C.elegans. We systematically performed endocytosis assays using these mutations and identified proteins involved in endocytic transport in the multicellular organism.

Research Products

• [Journal Article] モデル生物線虫から見た低分子量G蛋白質 Rab (2010)
  • Author(s): 安藤恵子
  • Journal Title: 細胞 42(3) Pages: 104-107
• [Journal Article] Two very long chain fatty acid acyl-CoA synthetase genes, acs-20 and acs-22, have roles in the cuticle surface barrier in Caenorhabditis elegans. (2010)
  • Author(s): Kage-Nakadai E, Kobuna H, Kimura M, 安藤恵子, Inoue T, Arai H, Mitani S
  • Journal Title: PLoS One. 5(1)
  • Peer Reviewed
• [Journal Article] モデル生物線虫から見た低分子量G蛋白質Rab (2010)
  • Author(s): 安藤恵子
  • Journal Title: 細胞 42(3) Pages: 104-107
• [Journal Article] FLR-2, the glycoprotein hormone alpha subunit, is involved in the neural control of intestinal funcitons in Caenorhabditis elegans. (2009)
  • Author(s): Oishi A, 安藤恵子, Mitani S, Mohri-Shiomi A, Kimura KD, Ishihara T, Katsura I.
  • Journal Title: Genes Cells. 14(10) Pages: 1141-54
  • Peer Reviewed
• [Journal Article] A Caenorhabditis elegans glycolipid-binding galectin functions in host defense againse bacterial infection. (2009)
  • Author(s): Ideo H, Fukushima K, 安藤恵子, Mitani S, Dejima K, Nomura K, Yamashita K.
  • Journal Title: J Biol Chem. 284(39) Pages: 26493-501
  • Peer Reviewed
• [Journal Article] Functional analysis of GS28, an intra-Golgi SNARE, in Caenorhabditis elegans. (2009)
  • Author(s): Maekawa M, Inoue T, Kobuna H, Nishimura T, 安藤恵子, Mitani S, Arai H.
  • Journal Title: Genes Cells. 14(8) Pages: 1003-13
  • Peer Reviewed
• [Journal Article] The ortholog of human solute carrier family 35 member B1(UDP-galactose transporter-related protein 1)is involved in maintenance of ER homeostasis and essential for larval development in Caenorhabditis elegans. (2009)
  • Author(s): Dejima K, Murata D, Mizuguchi S, Nomura KH, 安藤恵子, Mitani S, Kamiyama S, Nishihara S, Nomura K
  • Journal Title: FASEB J. 23(7) Pages: 2215-25
  • Peer Reviewed
• [Journal Article] Normal formation of a subset of intestinal granules in Caenorhabditis elegans requires ATP-binding cassette transporters HAF-4 and HAF-9, which are highly homologous to human lysosomal peptide transporter TAP-like. (2009)
  • Author(s): Kawai H, Tanji T, Shiraishi H, Yamada M, Iijima R, Inoue T, Kezuka Y, Ohashi K, Yoshida Y, Tohyama K, 安藤恵子, Mitani S, Arai H, Ohashi-Kobayashi A, Maeda M
  • Journal Title: Mol Biol Cell. 20(12) Pages: 2979-90
  • Peer Reviewed
• [Journal Article] Role of C. elegans TAT-1 protein in maintaining plasma membrane phosphatidylserine asymmetry. (2008)
  • Author(s): Darland-Ransom, M., et al.
  • Journal Title: Science 320 Pages: 528-531
  • Peer Reviewed
• [Journal Article] Systematic analysis of Secl/Munc18 family in membrane traffic of C. elegans (2008)
  • Author(s): Gengyo-Ando, K., et al.
  • Journal Title: The Journal of Physiological Sciences 58(Suppl. )
• [Journal Article] Caenorhabditis elegans mboa-7, a member of the MBOAT family, is required for selective incorporation of polyunsaturated fatty acids into phosphatidylinositol. (2008)
  • Author(s): Lee, H. C, et al.
  • Journal Title: Mol Biol Cell 19 Pages: 1174-1184
  • Peer Reviewed
• [Journal Article] IFT-81 and IFT-74 are required for intraflagellar transport in C. elegans (2007)
  • Author(s): Kobayashi, T., Gengyo-Ando, K., Ishihara, T., Katsura, I., Mitani, S.
  • Journal Title: Genes Cells 12 Pages: 593-602
  • Peer Reviewed
• [Journal Article] The SM protein VPS-45 is required for RAB-5-dependent endocytic transport in Caenorhabditis elegans (2007)
  • Author(s): Gengyo-Ando K, Kuroyanagi H, Kobayashi T, Murate M, Fujimoto K, Okabe S, Mitani S.
  • Journal Title: EMBO Reports 8(2) Pages: 152-7
  • Peer Reviewed
• [Journal Article] C. elegans mitochondrial factor WAH-1 promotes phosphatidylserine externalization in apoptotic cells through phospholipid scramblase SCRM-1. (2007)
  • Author(s): Wang X., et. al.
  • Journal Title: Nature Cell Biol 9・5 Pages: 541-549
  • Peer Reviewed
• [Journal Article] Control of sex-specific apoptosis in C. elegans by the BarH homeodomain protein CEH-30 and the transcriptional repressor UNC-37/Groucho (2007)
  • Author(s): Peden E., et. al.
  • Journal Title: Genes Dev. 21・23 Pages: 3195-207
  • Peer Reviewed
• [Journal Article] The SM protein VPS-45 is required for RAB-5-dependent endocytic transport in Caenorhabditis elegans (2007)
  • Author(s): Gengyo-Ando K., et. al.
  • Journal Title: EMBO Rep 8・2 Pages: 152-157
  • Peer Reviewed
• [Presentation] Functional genomics analysis of the C.elegans Sec1/Munc18 family involved in membrane traffic (2009)
  • Author(s): 安藤恵子、中台枝里子、三谷昌平
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-06-03
• [Presentation] メンブレントラフィクに関与する線虫Sec1/Munc18ファミリーのゲノム機能解析 (2009)
  • Author(s): 安藤恵子, 中臺枝里子, 三谷昌平
  • Organizer: 第61回日本細胞生物学会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] Functional genomics on membrane trafficking in C. elegans (2007)
  • Author(s): Keiko Gengyo-Ando, Shohei Mitani
  • Organizer: 日本遺伝学会国際シンポジウム
  • Place of Presentation: 岡山大学
  • Year and Date: 2007-09-22
• [Presentation] Functional genomics on membrane trafficking in C. elegans (2007)
  • Author(s): Gengyo-Ando, K., Mitani S.
  • Organizer: International Symposium "Gene Expression Control and Genome Evolution"
  • Place of Presentation: Okayama, Japan
• [Presentation] Physiological roles of the Sec1/Munc18 family in the endosomal/lysosomal system of C. elegans (2007)
  • Author(s): Gengyo-Ando, K., Kuroyanagi, H., Kobayashi, T., Murate, M., Fujimoto, K., Okabe, S., Mitani, S.
  • Organizer: 16th International C. elegans Meeting
  • Place of Presentation: Los Angeles, USA
• [Remarks] ホームページ等
  • URL: http://www.saitama-u.ac.jp/iron/hP-kenkyo/shinkou/nou.htm