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Analysis of the role of protein-protein interaction for the cell cycle progression using small molecule inhibitors

Research Project

Project/Area Number 19570193
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

WATANABE Nobumoto  The Institute of Physical and Chemical Research, 化合物バンク評価研究チーム, 先任研究員 (90221689)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords細胞周期 / Plk1 / PBD / タンパク質リン酸化 / タンパク質間相互作用 / タンパク質相互作用
Research Abstract

蛍光タンパク質標識PBDとその標的リン酸化ペプチドの結合を蛍光プレートリーダーで定量する系を構築し、PBD依存結合を阻害する小分子を化合物ライブラリーから探索しpurpurogallin(PPG)を見出した。PPGは、試験管内でPBDと標的タンパク質(Wee1)の結合を1μMでほぼ完全に阻害した。PPGを用いた解析で、PBDに依存した結合は、Plk1の細胞分裂における種々の役割の中でも、特に分裂中期における染色体の整列に最も重要な役割を有することが明らかになった。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (28 results)

All 2009 2008 2007 Other

All Journal Article (15 results) (of which Peer Reviewed: 12 results) Presentation (12 results) Remarks (1 results)

  • [Journal Article] Deficiency in chromosome congression by the inhibition of PLK1 polo box domain-dependent recognition2009

    • Author(s)
      Watanabe, N (7人中1番目)
    • Journal Title

      J Biol. Chem. 284

      Pages: 2344-2353

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Polo-like kinase 1 phosphorylates and regulates Bcl-xL during pironetin-induced apoptosis2009

    • Author(s)
      Watanabe, N (7人中6番目)
    • Journal Title

      Oncogene 28

      Pages: 107-116

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 癌細胞の細胞周期異常2009

    • Author(s)
      渡邉信元, 長田裕之
    • Journal Title

      日本臨牀 67

      Pages: 71-76

    • Related Report
      2008 Final Research Report
  • [Journal Article] Polo-like kinase 1 phosphorylates and regulates Bcl-x_L during pironetin-induced apoptosis2009

    • Author(s)
      Tamura, Y, et al
    • Journal Title

      Oncogene, 28, 107-116 (2009) 28

      Pages: 107-116

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deficiency in chromosome congression by the inhibition of PLK1 polo box domain-dependent recognition2009

    • Author(s)
      Watanabe, N, et al
    • Journal Title

      J Biol. Chem. 284

      Pages: 2344-2353

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 癌細胞の細胞周期異常2009

    • Author(s)
      渡邉信元
    • Journal Title

      日本臨牀 67

      Pages: 71-76

    • Related Report
      2008 Annual Research Report
  • [Journal Article] The regulatory βsubunit of protein kinase CK2 regulates cell-cycle progression at the onset of mitosis2008

    • Author(s)
      Watanabe, N (5人中4番目)
    • Journal Title

      Oncogene 27

      Pages: 4986-4997

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Human immunodeficiency virus type 1 Vpr binds to the N lobe of the Wee1 kinase domain and enhances kinase activity for CDC22008

    • Author(s)
      Watanabe, N (4人中2番目)
    • Journal Title

      J Virol. 82

      Pages: 5672-5682

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fbxw7 acts as an E3 Ubiquitin ligase that targets c-Myb for nemo-like kinase (NLK)-induced degradation2008

    • Author(s)
      Watanabe, N (6人中4番目)
    • Journal Title

      J Biol. Chem. 283

      Pages: 30540-30548

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Wee12008

    • Author(s)
      Watanabe, N
    • Journal Title

      UCSD-Nature Molecule Pages

      Pages: 0-0

    • URL

      http://www.signaling-gateway.org/molecule/query?afcsid=A002371

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The regulatory β-subunit of protein kinase CK2 regulates cell-cycle progression at the onset of mitosis2008

    • Author(s)
      Yde, CW, et al
    • Journal Title

      Oncogene 27

      Pages: 4986-4997

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Human immunodeficiency virus type 1 Vpr binds to the N lobe of the Wee1 kinase domain and enhances kinase activity for CDC22008

    • Author(s)
      Kamata, M, et al
    • Journal Title

      J Virol. 82

      Pages: 5672-5682

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fbxw7 acts as an E3 Ubiquitin ligase that targets c-Myb for nemo-like kinase (NLK)-induced degradation2008

    • Author(s)
      Kanei-Ishii, C, et al
    • Journal Title

      J Biol. Chem. 283

      Pages: 30540-30548

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Wee12008

    • Author(s)
      Watanabe, N
    • Journal Title

      UCSD-Nature Molecule Pages http://www.signaling-gateway.org/molecule/query?afcsid=A002371

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 染色体複製から分裂開始までを監視するチェックポイント経路-その活性化と不活性化の分子機構-2007

    • Author(s)
      渡辺 信元
    • Journal Title

      実験医学 25

      Pages: 662-670

    • Related Report
      2007 Annual Research Report
  • [Presentation] SCFbeta-TrCPによるCdc25Bの制御2008

    • Author(s)
      内田 早苗
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会 (BMB2008)
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-11
    • Related Report
      2008 Final Research Report
  • [Presentation] SCFbeta-TrCPによるCdc25Bの制御2008

    • Author(s)
      内田早苗
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会(BMB2008)
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] 小分子阻害剤を用いた細胞分裂におけるPBD依存性結合の役割の解析2008

    • Author(s)
      渡邉 信元
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会 (BMB2008)
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-10
    • Related Report
      2008 Final Research Report
  • [Presentation] 小分子阻害剤を用いた細胞分裂におけるPBD依存性結合の役割の解析2008

    • Author(s)
      渡邉信元
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会(BMB2008)
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] 小分子阻害剤によるPlk1ポロボックス依存性結合の細胞分裂進行における役割の解析2008

    • Author(s)
      渡邉 信元
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-10-28
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Small molecule inhibitors of phosphorylation dependent protein-protein interactions2008

    • Author(s)
      渡邉 信元
    • Organizer
      Friday seminar by USM visiting professors
    • Place of Presentation
      Univ. Sains, Malaysia, Penang
    • Year and Date
      2008-07-25
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Development of a screening system for HIV-1 Vpr Inhibitors and the study of its mechanism of action2008

    • Author(s)
      Eugene Ong
    • Organizer
      4th Korea-Japan Chemical Biology Symposium
    • Place of Presentation
      日光
    • Year and Date
      2008-05-21
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] 細胞分裂期進行におけるPlk1ポロボックスドメイン依存性結合の役割の化学生物学的解析2008

    • Author(s)
      渡邉信元
    • Organizer
      日本ケミカルバイオロジー研究会第3回年会
    • Year and Date
      2008-05-19
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Plk1のポロボックスドメイン依存結合は分裂中期染色体の速やかな整列に必要である2007

    • Author(s)
      渡辺 信元
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会・合同大会
    • Place of Presentation
      パシフィコ横浜,横浜
    • Year and Date
      2007-12-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] Small Molecule inhibitors for Polo Box Domain Dependent Recognition2007

    • Author(s)
      Nobumoto Watanabe
    • Organizer
      AACR-NCI-EORTC International Conference Molecular Targets and Cancer Therapeutics
    • Place of Presentation
      Moscone West ConventionCenter,San Francisco,米国
    • Year and Date
      2007-10-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] Screening for small molecule inhibitors of polo box domain dependent recognition2007

    • Author(s)
      Nobumoto Watanabe
    • Organizer
      66th Annual Meeting of the Japanese Cancer Association
    • Place of Presentation
      パシフィコ横浜,横浜
    • Year and Date
      2007-10-05
    • Related Report
      2007 Annual Research Report
  • [Presentation] Identification of Small Molecule Inhibitors of Polo Box Domain Dependent Recognition2007

    • Author(s)
      Nobumoto Watanabe
    • Organizer
      the 5th Salk Institute Cell Cycle Meeting
    • Place of Presentation
      Salk Inst.San Diego,米国
    • Year and Date
      2007-07-15
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.antibiotics.riken.jp

    • Related Report
      2008 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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