Screening for nuclear membrane proteins in Schizosaccharomyces pombe to identify novel factors involved in chromosome arrangement

• Project/Area Number: 19570194
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: National Institute of Information and Communications Technology
• Principal Investigator: CHIKASHIGE Yuji National Institute of Information and Communications Technology, 未来ICT研究センター・バイオICTグループ, 主任研究員 (60359081)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 核構造 / Schizosaccharomyces pombe / 核膜 / 減数分裂 / テロメア / 染色体

Research Abstract

染色体配置における核膜のはたす役割の分子機構を明らかにする目的で、本研究では、分裂酵母において、染色体の核内配置に関与する因子を探索し、新規の核膜タンパク質を2個同定し、それぞれBqt3, Bqt4と命名した。両タンパク質は、核内膜において複合体を形成し、核内膜タンパク質としては、初めて、染色体末端と核膜との相互作用を介在する因子であることを明らかにした。

Research Products

• [Journal Article] Codon usage bias is correlated with gene expression levels in the fission yeast Schizosaccharomyces pombe (2009)
  • Author(s): Hiraoka Y, Kawamata K, Haraguchi T and Chikashige Y
  • Journal Title: Genes to Cells 14 Pages: 499-509
  • Peer Reviewed
• [Journal Article] Codon usage bias is correlated with gene expression levels in the fission yeast Schizosaccharomyces pombe (2009)
  • Author(s): Yasushi Hiraoka
  • Journal Title: Genes to Cells 14(未定)
  • Peer Reviewed
• [Journal Article] Gene Expression and Distribution of Swi6 in Partial Aneuploids of the Fission Yeast Schizosaccharomyces pombe (2007)
  • Author(s): Chikashige Y, Tsutsumi C, Okamasa K, Yamane M, Nakayama J, Niwa O, Haraguchi T, Hiraoka Y.
  • Journal Title: Cell Struct. Funct. 32(2) Pages: 149-61
  • NAID: 40016192027
  • Peer Reviewed
• [Journal Article] Another way to move chromosomes. (2007)
  • Author(s): Chikashige, Y., Haraguchi, T., and Hiraoka, Y.
  • Journal Title: Chromosoma 116(6) Pages: 497-505
  • Peer Reviewed
• [Journal Article] Gene expression and distribution of Swi6 in partial aneuploids of the fission yeast Schizosaccharomyces pombe (2007)
  • Author(s): Chikashige Yuji
  • Journal Title: Cell Structure and Function 32 Pages: 149-161
  • NAID: 40016192027
  • Peer Reviewed
• [Journal Article] Another way to move chromosomes. (2007)
  • Author(s): Chikashige Yuji
  • Journal Title: Chromosoma 116 Pages: 497-505
  • Peer Reviewed
• [Presentation] 核内膜タンパク質Bqt3, Bqt4 は、テロメアを核膜へ繋ぎとめて、減数分裂期ブーケ配置形成を可能にする。 (2008)
  • Author(s): 近重 裕次
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸・日本
  • Year and Date: 2008-12-12
• [Presentation] 核内膜タンパク質Bqt3,Bqt4は、テロメアを核膜へ繋ぎとめて、減数分裂期ブーケ配置形成を可能にする (2008)
  • Author(s): 近重裕次
  • Organizer: 第31回日本分子生物学会年会 第81回日本生化学会大会、合同大会
  • Place of Presentation: 神戸、日本
  • Year and Date: 2008-12-12
• [Presentation] A group of proteins that work for telomere positioning in fission yeast (2008)
  • Author(s): Yasushi Hiraoka
  • Organizer: Dynamic Organization of Nuclear Function Cold Spring Harbor Meeting
  • Place of Presentation: Cold Spring Harbor, USA
  • Year and Date: 2008-09-18
• [Presentation] A group of proteins that work for telomere positioning in fission yeast (2008)
  • Author(s): Yasushi Hiraoka
  • Organizer: Cold Spring Harbor Meeting Dynamic Organization of Nuclear Function
  • Place of Presentation: Cold Spring Harbor, 米国
  • Year and Date: 2008-09-18
• [Presentation] Inner nuclear membrane proteins required for the chromosomal bouquet arrangement in Schizosaccharomyces pombe (2008)
  • Author(s): Yuji Chikashige
  • Organizer: EMBO CONFERENCE SERIES "Telomeres and the DNA damage response"
  • Place of Presentation: Villars-sur-Ollon, Switzerland
  • Year and Date: 2008-09-15
• [Presentation] Inner nuclear membrane proteins required for the chromosomal bouquet arrangement in Schizosaccharomyces pombe (2008)
  • Author(s): Yuji Chikashige
  • Organizer: EMBO CONFERENCE SERIES "Telomeres and the DNA damage response"
  • Place of Presentation: Villars-sur-Ollon, スイス
  • Year and Date: 2008-09-15
• [Presentation] Inner nuclear membrane proteins required for the chromosomal bouquet arrangement in Schizosaccharomyces pombe (2008)
  • Author(s): 近重 裕次
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜・日本
  • Year and Date: 2008-06-29
• [Presentation] Inner nuclear membrane proteins required for the chromosomal bouquet arrangement in Schizosaccharomyces pombe (2008)
  • Author(s): 近重裕次
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜、日本
  • Year and Date: 2008-06-29
• [Presentation] 分裂酵母減数分裂期ブーケ形成における核内膜タンパク質Bqt3,Bqt4の機能 (2008)
  • Author(s): 近重 裕次
  • Organizer: 第25回染色体ワークショップ
  • Place of Presentation: 熱海・日本
  • Year and Date: 2008-01-31
• [Presentation] 分裂酵母減数分裂期ブーケ形成における核内膜タンパク質Bqt3,Bqt4の機能 (2008)
  • Author(s): 近重 裕次
  • Organizer: 第25回染色体ワークショップ
  • Place of Presentation: 熱海、日本
  • Year and Date: 2008-01-31
• [Presentation] Nuclear membrane proteins required for meiotic SPB-telomere cluster formation in Schizosaccharomyces pombe (2007)
  • Author(s): Yuji Chikashige
  • Organizer: 8th European Meiosis Meeting
  • Place of Presentation: 葉山・日本
  • Year and Date: 2007-09-15
• [Presentation] Nuclear membrane proteins required for meiotic SPB-telomere cluster formation in Schizosaccharomyces pombe (2007)
  • Author(s): Chikashige Yuji
  • Organizer: 8th European Meiosis Meeting
  • Place of Presentation: 葉山、日本
  • Year and Date: 2007-09-15
• [Presentation] Fission Yeast Genes Involved in Meiotic SPB-Telomere Cluster Formation (2007)
  • Author(s): Yuji Chikashige
  • Organizer: 4th International Fission Yeast Meeting
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2007-06-13
• [Presentation] FISSION YEAST GENES INVOLVED IN MEIOTIC SPB-TELOMERE CLUSTER FORMAT (2007)
  • Author(s): Chikashige Yuji
  • Organizer: 4th International Fission Yeast Meeting
  • Place of Presentation: コペンハーゲン、デンマーク
  • Year and Date: 2007-06-13
• [Presentation] Fission yeast genes involved in meiotic SPB-telomere cluster formation (2007)
  • Author(s): Yuji Chikashige
  • Organizer: 第40回日本発生生物学会・第59回日本細胞生物学会、合同大会
  • Place of Presentation: 福岡・日本
  • Year and Date: 2007-05-29
• [Presentation] Fission yeast genes involved in meiotic SPB-telomere cluster formation (2007)
  • Author(s): Chikashige Yuji
  • Organizer: 第40回日本発生生物学会・第59回日本細胞生物学会合同大会
  • Place of Presentation: 福岡、日本
  • Year and Date: 2007-05-29
• [Remarks]
  • URL: http://www2.nict.go.jp/w/w103/w131103/CellMagic/index.html
• [Remarks]
  • URL: http://www2.nict.go.jp/w/w103/w131103/CellMagic/index.html