• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Induction of bone formation with BMP in amputated mouse limbs

Research Project

Project/Area Number 19570197
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionTohoku University

Principal Investigator

IDE H Iroyuki  Tohoku University, 大学院・生命科学研究科, 名誉教授 (70022704)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords四肢の再生 / マウス / 骨形成タンパク / 骨パターン / 繊維芽細胞成長因子 / マウス四肢 / 再生 / 骨形成タンパク質 / パターン形成
Research Abstract

有尾両生類では切断された四肢の完全な再生が起こるのに対し、マウスなど哺乳類では四肢の再生はほとんど起こらない。指先の切断部で再生が見られるのと、より基部側の切断部で、軟骨の肥大が起こり、それが骨化するだけであった。本研究の結果、新生仔マウス四肢の切断後に新しく軟骨形成や骨化を起こすにはBMP-7またはBMP-2が必要で、これらBMPの濃度に依存して、骨パターンに類似の構造が形成されることが明らかになった。軟骨形成は切断部で広く起こり、急速に細胞塊を作り、骨化してゆくと考えられる。一方、BMP は主に切り株とは独立の構造を誘導し、これは軟骨形成を経て作られた。BMPの適当な濃度、キャリアー(徐放剤)を選択することによって、さらに他の成長因子を加えることによって、四肢の骨パターンに近いものが形成された。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (12 results)

All 2009 2008 2007

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (5 results) Book (2 results)

  • [Journal Article] The autopod ; its formation during limb development2009

    • Author(s)
      Tamura, K., Yonei-Tamura, S., Yano T., Ide. H
    • Journal Title

      Development Growth and Differentiation 50

      Pages: 177-187

    • NAID

      10024399869

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The autopod : its formation during limb development2008

    • Author(s)
      Tamura, K. Yonei-Tamura, S, Yano T., Ide
    • Journal Title

      Develop. Growth Differ 50

      Pages: 177-187

    • NAID

      10024399869

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regeneration potency of mouse limbs2007

    • Author(s)
      Masaki, H., Ide, H.
    • Journal Title

      Develop. Growth Differ 49

      Pages: 89-98

    • NAID

      10018878304

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Correlation between Shh expression and DNA methylation status of the limb-specific Shh enhancer region during limb regeneration in amphibians2007

    • Author(s)
      Yakushiji, N., Suzuki, M., Satoh, A., Sagai T., Shiroishi, T., Kobayashi, H., Sasaki, H., Ide, H., Tamura, K.
    • Journal Title

      Develop. Biol. 312

      Pages: 171-182

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regeneration Potency of Mouse Limbs2007

    • Author(s)
      Masaki, H.
    • Journal Title

      Development Growth & Differentiation 49

      Pages: 89-98

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] BMP therapy. Regeneration of forearm2009

    • Author(s)
      Hiroyuki Ide
    • Organizer
      DARPA meeting, San Antonio
    • Place of Presentation
      U.S.A.
    • Year and Date
      2009-04-17
    • Related Report
      2008 Final Research Report
  • [Presentation] BMP and regeneration of forelimb elements2008

    • Author(s)
      Hiroyuki Ide
    • Organizer
      DARPA meeting
    • Place of Presentation
      U.S.A.
    • Year and Date
      2008-10-20
    • Related Report
      2008 Final Research Report
  • [Presentation] BMP and Regeneration of Forearm elements2008

    • Author(s)
      Hiroyuki Ide
    • Organizer
      DARPA Kickoff Meeting
    • Place of Presentation
      Boar's Head Inn (ヴァージニア州、米国)
    • Year and Date
      2008-10-19
    • Related Report
      2008 Annual Research Report
  • [Presentation] マウス四肢の再生能2007

    • Author(s)
      井出宏之、正木英樹、佐藤伸、薬師寺那由, 他
    • Organizer
      日本動物学会78回大会
    • Year and Date
      2007-09-25
    • Related Report
      2008 Final Research Report
  • [Presentation] マウス四肢の再生能2007

    • Author(s)
      井出宏之
    • Organizer
      日本動物学会
    • Place of Presentation
      弘前大学
    • Year and Date
      2007-09-20
    • Related Report
      2007 Annual Research Report
  • [Book] 四肢のパターン形成(浅島誠、武田洋幸編)2007

    • Author(s)
      井出宏之
    • Publisher
      培風館
    • Related Report
      2008 Final Research Report
  • [Book] 四肢のパターン形成 (発生の第5章)2007

    • Author(s)
      井出宏之
    • Publisher
      培風館
    • Related Report
      2007 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi