Functional analysis of novel factors regulated by inner nuclear membrane proteins

• Project/Area Number: 19570199
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Nippon Medical School (2008); Akita University (2007)
• Principal Investigator: SHIN-ICHI Osada Nippon Medical School, 医学部, 助教 (00244484)
• Co-Investigator(Kenkyū-buntansha): AKIHIKO Ishimura 金沢大学, がん研究所, 助教 (80375261)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 発生・分化 / シグナル伝達 / 核膜蛋白質 / 左右非対称性 / 発現調節 / 細胞・組織 / マイクロアレイ

Research Abstract

本研究では、核膜内膜蛋白質の一つMAN1の機能を「シグナルの制御因子」という観点から解析した。Man1欠損胚において心臓の形態異常が現れるメカニズムを調べる過程で、Man1欠損胚では、体軸の左右非対称性の決定に関わるNodal経路が異常に活性化するために、左右軸の形成が異常になることを見出した。本研究により、MAN1がNodal経路の制御を介して左右軸の決定に関わることが世界で初めて明らかになった。

Research Products

• [Journal Article] Man1, an inner nuclear membrane protein,regulates left-right axis formation by controlling Nodal signaling in a node-independent manner (2008)
  • Author(s): Akihiko Ishimura, Shinsuke Chida, Shin-Ichi Osada
  • Journal Title: Dev.Dyn. 237 Pages: 3565-3576
  • Peer Reviewed
• [Journal Article] Manl, an inner nuclear membrane protein, regulates left-right axis for mation by controlling Nodal signaling in a node-independent manner. (2008)
  • Author(s): Akihiko Ishimura
  • Journal Title: Dev. Dyn. 237 Pages: 3565-3576
  • Peer Reviewed
• [Journal Article] Integral nuclear membrane proteins in vertebrate development (2007)
  • Author(s): Shin-Ichi Osada
  • Journal Title: Func. Dev. Embryol 1 Pages: 14-25
  • Peer Reviewed
• [Journal Article] Integral nuclear membrane proteins in vertebrate development (2007)
  • Author(s): Shin-lchi Osada
  • Journal Title: Functional Development and Embryology 1 Pages: 14-25
  • Peer Reviewed
• [Presentation] A role for MAN1, an inner nuclear membrane protein, in left-right axis formation (2007)
  • Author(s): 石村昭彦、千田進介、長田真一
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜市、パシフィコ横浜
  • Year and Date: 2007-12-13
• [Presentation] A role for MAN1, an inner nuclear membrane protein, in left-right axis formation. (2007)
  • Author(s): 石村 昭彦
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜市,パシフィコ横浜
  • Year and Date: 2007-12-13
• [Presentation] Man1, an inner nuclear membrane protein, regulates left-right axis formation by modulating Nodal signaling (2007)
  • Author(s): 石村昭彦、千田進介、長田真一
  • Organizer: 第40回日本発生生物学会・第59回日本細胞生物学会合同大会
  • Place of Presentation: 福岡市
  • Year and Date: 2007-05-29
• [Presentation] Man1, an inner nuclear membrane protein, regulates left-right axis for mation by modulating Nodal signaling. (2007)
  • Author(s): 石村 昭彦
  • Organizer: 第40回日本発生生物学会・第59回日本細胞生物学会合同大会
  • Place of Presentation: 福岡市,福岡国際会議場
  • Year and Date: 2007-05-29