Developmental mechanism of ionocytes in the skin of zebfaish larvae to acclimate fresh water

• Project/Area Number: 19570201
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: HOSHIJIMA Kazuyuki Tokyo Institute of Technology, バイオフロンティアセンター, バイオフロンティア研究教員 (70397032)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 環境 / 発現制御 / 発生・分化 / イオンホメオスタシス / ホメオスタシス / zinc-finger nuclease / マイクロアレイ

Research Abstract

淡水魚の体表に発達する表層イオン代謝細胞は,淡水中より様々なイオンを取り込み,体内のイオン濃度を一定に保つために必要不可欠な細胞群と考えられてきた。我々はこれまでにfoxi3a遺伝子が表層イオン代謝細胞の発生分化に必須であることを明らかにしてきたが,本研究ではさらにfoxi1遺伝子,deltaC遺伝子,およびgcm2遺伝子がその細胞分化形成に関わっていることを示し,それら遺伝子間相互作用,作用機序を明らかにした。

Research Products

• [Journal Article] Identification of zebrafish FXYD protein that is highly expressed in ion-transporting epithelium of the gill and skin and its possible role in ion homeostasis. (2010)
  • Author(s): Saito, K., Nakamura, N., Ito, Y., Hoshijima, K., Esaki, M., Zhao, B., Hirose, S.
  • Journal Title: Frontiers in Aquatic Physiology (in press)
  • Peer Reviewed
• [Journal Article] Identification of zebrafish FXYD protein that is highly expressde inion-transporting epithelium of the gill and skin and its possible role in ionhomeostasis. (2010)
  • Author(s): Saito, K., Nakamura, N., Ito, Y., Hoshijima, K., Esaki, M., Zhao, B., Hirose, S.
  • Journal Title: Frontiers in Aquatic Physiology (in press)
  • Peer Reviewed
• [Journal Article] Chromosomal position mediates spinal cord expression of a dbx1a enhancer (2009)
  • Author(s): Gribble, S.L., Nikolaus, O.B., Carver, M.S., Hoshijima, K., Dorsky, R.I.
  • Journal Title: Developmental Dynamics 238 Pages: 2929-2935
  • Peer Reviewed
• [Journal Article] Mechanism of development of ionocytes rich in vacuolar-type H+-ATPase in the skin of zebrafish larvae. (2009)
  • Author(s): Esaki, M., Hoshijima, K., Nakamura, N., Munakata, K., Tanaka, M., Ookata, K., Asakawa, K., Kawakami, K., Wang, W., Weinberg, E.S., Hirose, S.
  • Journal Title: Developmental Biology 320 Pages: 116-120
  • Peer Reviewed
• [Journal Article] Chromosomal position mediates spinal cord expression of a dbxla enhancer. (2009)
  • Author(s): Gribble, S.L., Nikolaus, O.B., Carver, M.S., Hoshijima, K., Dorsky, R.I.
  • Journal Title: Developmental Dynamics 238 Pages: 2929-2935
  • Peer Reviewed
• [Journal Article] Mechanism of development of ionocytes rich in vacuolar-type H^+-ATPase in the skin of zebrafish larvae (2009)
  • Author(s): Esaki, M., Hoshijima, K., Nakamura, N., Munakata, K., Tanaka, M., Ookata, K., Asakawa, K., Kawakami, K., Wang, W., Weinberg, E. S., Hirose, s.
  • Journal Title: Developmental Biology 320 Pages: 116-120
  • Peer Reviewed
• [Journal Article] Zebrafish early cardiac connexin, 36. 7/Ecx regulates heart morphogenesis by establishing nkx2. 5 expression. (2008)
  • Author(s): Sultana, N.,, Nag, K., Hoshijima, K., Kawakami, A., Laird, D.W., Hiros e, S.
  • Journal Title: Proc. Natl. Acad. Sci. USA. 105 Pages: 623-631
  • Peer Reviewed
• [Journal Article] Zebrafish early cardiac connexin, 36.7/Ecx regulates heart morphogenesis by establishing nkx2.5 expression. (2008)
  • Author(s): N. Sultana, K. Nag, K. Hoshijima, A. Kawakami, D. W. Laird, S. Hirose
  • Journal Title: Proc. Natl. Acad. Sci. USA. 105 Pages: 623-631
  • Peer Reviewed
• [Journal Article] Localization of ammonia transpor ter Rhcg1 in mitochondrion-rich cells of yolk sac, gill, and kidney of zebrafish a nd its ionic strength-dependent expressio n. (2007)
  • Author(s): Nakada, T., Hoshijima, K., Esaki, M., Nagayoshi, S., Kawakami, K., Hiro se, S.
  • Journal Title: Am. J. Physiol. Regul. Integr. Comp. Physiol. 293
  • Peer Reviewed
• [Journal Article] Expression of endocrine genes in zebrafish larvae in response to environmental salinity. (2007)
  • Author(s): Hoshijima, K., Hirose, S.
  • Journal Title: Journal of Endocrinology 193 Pages: 481-491
  • Peer Reviewed
• [Journal Article] Expression of endocrine genes in zebrafish larvae in response to environ mental salinity. (2007)
  • Author(s): K. Hoshijima, and S. Hirose
  • Journal Title: Journal of Endocrinology 193 Pages: 481-491
  • Peer Reviewed
• [Journal Article] Localization of ammonia transporter Rhcgl in mitochondrion-rich cells of yolk sac, gill, and kidney of zebrafish and its ionic strength-dependent expression. (2007)
  • Author(s): T. Nakada, K. Hoshijima, M. Esaki, S. Nagayoshi, K. Kawakami, and S. Hirose
  • Journal Title: Am. J. Physiol. Regul. Integr. Comp. Physiol. 293
  • Peer Reviewed
• [Presentation] ZFN construction using OPEN Pool in B1H system (2010)
  • Author(s): Hoshijima, K., Dahlem, T., Grunwald, D.
  • Organizer: Zebrafish Phenome Project Workshop
  • Place of Presentation: Bethesda, Maryland, USA
  • Year and Date: 2010-03-13
• [Presentation] ZFN construction using OPEN Pools and a Bacterial One-Hybrid selection system (2010)
  • Author(s): Kazuyuki Hoshijima
  • Organizer: Zebrafish Phenome Project Workshop
  • Place of Presentation: Bethesda, Maryland, USA
  • Year and Date: 2010-03-13
• [Remarks]
  • URL: http://www.hirose.bio.titech.ac.jp/index.html