Identification and functional analysis of genes that affect the formation of endoderm and endodermal organs

• Project/Area Number: 19570204
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Hiroshima University
• Principal Investigator: KIKUCHI Yutaka Hiroshima University, 大学院・理学研究科, 教授 (20286438)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ゼブラフィッシュ / 内胚葉性器官 / 器官形成 / 変異体 / 原因遺伝子 / 突然変異体

Research Abstract

本研究では、内胚葉性器官、特に消化器官形成の異常が顕著な新規変異体morendo(mor)とlegato(leg)変異体の解析を中心に研究を進め、mor変異体の原因遺伝子の同定に成功した。現在Morタンパク質の機能に関して研究を進めている段階である。また、leg変異体に関しては、原因遺伝子が含まれるゲノム領域の絞り込みに成功した。今後の解析により、原因遺伝子を同定することが出来ると考えている。
更に私は、内胚葉細胞が特異的に蛍光を発するトランスジェニックゼブラフィッシュTg(sox17 : EGFP)を用いることにより、原腸陥入期における内胚葉細胞の運動がケモカインシグナル(SDF1/Cxcr4シグナル)により制御されていることを明らかにし、論文発表(Mizoguchi et.al., 2008)を行った。

Research Products

• [Journal Article] Sdf1/Cxcr4 signaling controls the dorsal migration of endodermal cells during zebrafish gastrulation (2008)
  • Author(s): Mizoguchi, T., Verkade, H., Heath, J., Kuroiwa, A. and Kikuchi Kikuchi, Y.
  • Journal Title: Development 135 Pages: 2521-2529
  • Peer Reviewed
• [Journal Article] Translational enhancement of recombinant protein synthesis in transgenic silkworms by a 5'- untranslated region of polyhedrin gene of Bombyx mori Nucleopolyhedrovirus (2008)
  • Author(s): Iizuka, M., Tomita, M., Shimizu, K., Kikuchi, Y. and Yoshizato, K.
  • Journal Title: Journal of Bioscience and Bioengineering 105 Pages: 595-603
  • NAID: 110006792535
  • Peer Reviewed
• [Journal Article] Identification and characterization of nucleoplasmin 3 as a histone-binding protein in embryonic stem cells (2008)
  • Author(s): Motoi, N., Suzuki, K., Hirota, R., Johnson, P., Oofusa, K., Kikuchi, Y. and Yoshizato, K.
  • Journal Title: Development Growth & Differentiation 50 Pages: 307-320
  • NAID: 10021096598
  • Peer Reviewed
• [Journal Article] Extensive expansion and diversification of the chemokine gene family in zebrafish : identification of a novel chemokine subfamily CX (2008)
  • Author(s): Nomiyama, H., Hieshima, K., Osada, N., Kato-Unoki, Y., Otsuka-Ono, K., Takegawa, S., Izawa, T., Yoshizawa, A., Kikuchi, Y. Y., Tanase, S., Miura, R., Kusuda, J., Nakao, M., and Yoshie, O.
  • Journal Title: BMC Genomics 9 Pages: 222-222
  • Peer Reviewed
• [Journal Article] Mtx2 directs zebrafish morphogenetic movements during epiboly by regulating microfilament formation (2008)
  • Author(s): Wilkins, S., Yoong, S., Verkade, H., Mizoguchi, T., Plowman, S., Hancock, J., Kikuchi, Y. Y., Heath, J. and Perkins, A.
  • Journal Title: Developmental Biology 314 Pages: 12-22
  • Peer Reviewed
• [Journal Article] 内胚葉分化にかかわるシグナル、転写因子群の機能 (2008)
  • Author(s): 溝口貴正, 菊池裕
  • Journal Title: 内分泌・糖尿病科 26 Pages: 106-114
• [Journal Article] Sdf1/Cxcr4シグナルはゼブラフィッシュの原腸陥入期において内胚葉細胞の背側への移動を制御する (2008)
  • Author(s): 溝口貴正, 菊池裕
  • Journal Title: 細胞工学 27 Pages: 1160-1161
• [Journal Article] Sdfl/Cxcr4 signaling controls the dorsal migration of endodermal cells during zebrafish gastrulation. (2008)
  • Author(s): Mizoguchi, T., Verkade, H., Heath, J., Kuroiwa, A., Kikuchi. Y.
  • Journal Title: Development 135 Pages: 2521-2529
  • Peer Reviewed
• [Journal Article] Sdfl/Cxcr4シグナルはゼブラフィッシュの原腸陥入期において内胚葉細胞の背側への移動を制御する (2008)
  • Author(s): 溝口貴正, 菊池 裕
  • Journal Title: 細胞工学 27 Pages: 1160-1161
• [Journal Article] Mtx2 directs zebrafish morphogenetic movements during epiboly byregulating microfilament formation. (2008)
  • Author(s): Wilkins, S.J., Yoong, S., Verkade, H., Mizoguchi, T., Plowman, S.J., Hancock, J.F., Kikuchi. Y., Heath, J.K. and Perkins, A.C.
  • Journal Title: Developmental Biology 314 Pages: 12-22
  • Peer Reviewed
• [Journal Article] Identification and characterization of nucleoplasmin 3 as a histone-binding protein in embryonic stem cells. (2008)
  • Author(s): Motoi, N., Suzuki, K., Hirota, R., Johnson, P., Oofusa, K., Kikuchi, Y. and Yoshizato, K.
  • Journal Title: Developmental Growth & Differentiation (in press)
  • NAID: 10021096598
  • Peer Reviewed
• [Journal Article] Translational enhancement of recombinant protein synthesis in transgenic silkworms by a 5'-untranslated region of polyhedrin gene of Bombyx mori Nucleopolyhedrovirus. (2008)
  • Author(s): Iizuka, M., Tomita, M., Shimizu, K., Kikuchi, Y. and Yoshizato, K.
  • Journal Title: Journal of Bioscience and Bioengineering (in press)
  • NAID: 110006792535
  • Peer Reviewed
• [Journal Article] Initial specification of the epibranchial placode in zebrafish embryos depends on the fibroblast growth factor signal (2007)
  • Author(s): Nikaido, M., Doi, K., Shimizu, T., Hibi, M., Kikuchi, Y. and Yamasu, K.
  • Journal Title: Developmental Dynamics 236 Pages: 564-571
  • Peer Reviewed
• [Journal Article] 特集"内胚葉分化の分子メカニズム"(編集 ; 菊池裕)序論-器官形成から再生を目指して- (2007)
  • Author(s): 菊池裕
  • Journal Title: 蛋白質核酸酵素 52 Pages: 101-104
• [Journal Article] 脊椎動物における内胚葉形成の分子機構 (2007)
  • Author(s): 溝口貴正, 菊池裕
  • Journal Title: 蛋白核酸酵素 52 Pages: 105-111
• [Journal Article] Cyp26 enzymes function in endoderm to regulate pancreatic field size.
  • Author(s): Kinkel, M. D., Sefton, E. M., Kikuchi. Y., Mizoguchi, T., Andrea B. Ward, A. B., Prince, V. E.
  • Journal Title: Proc. Natl. Acad. Sci. USA (in press)
  • Peer Reviewed
• [Presentation] ゼブラフィッシュ原腸陥入期における内胚葉細胞の移動機構 (2007)
  • Author(s): 溝口貴正, 黒岩厚, 菊池裕
  • Organizer: 第30回日本分子生物学会ワークショップ招待講演
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2007-12-14
• [Presentation] ゼブラフィッシュ原腸陥入期における内胚葉細胞の移動機構 (2007)
  • Author(s): 菊池 裕
  • Organizer: 日本分子生物学会(ワークショップ)
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2007-12-14