Studies on the molecular mechanisms underlying mouse primordial germ cell localization

• Project/Area Number: 19570209
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Kumamoto University
• Principal Investigator: TANAKA Satomi Kumamoto University, 発生医学研究センター, 助教 (10321944)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 生殖細胞 / 移動 / Ifitm1 / Ifitm3 / マウス / 始原生殖細胞 / 培養マウス胚 / Ifitm1遺伝子 / Ifitm3遺伝子 / 初期胚

Research Abstract

マウス始原生殖細胞(PGC)の移動におけるIfitmファミリー遺伝子の役割について解析を進めた。Ifitm1の異所的発現は、PGC局在に対して排他的に働いた。また、PGCの移動に異常が観察されるミュータントマウスでもIfitm1の発現異常が観察された。siRNAを用いた培養マウス胚でのIfitm1ノックダウン実験では、PGCの局在異常が観察された。

Research Products

• [Journal Article] Draxin a Repulsive Guidance Protein for Spinal Cord and Forebrain Commissures (2009)
  • Author(s): Shahidul M. Islam, Yohei Shinmyo, Tatsuya Okafuji, Yuhong Su, Iftekhar Bin Naser, Giasuddin Ahmed, Sanbing Zhang, Sandy Chen, Kunimasa Ohta, Hiroshi Kiyonari, Takaya Abe, Satomi Tanaka, Ryuichi Nishinakamura, Toshio Terashima, Toshio Kitamura, Hideaki Tanaka
  • Journal Title: Science 323 Pages: 388-393
  • Peer Reviewed
• [Journal Article] Draxin, a Repulsive Guidance Protein for Spinal Cord and Forebrain Commissures. (2009)
  • Author(s): Shahidul M. Islam
  • Journal Title: Science 323 Pages: 388-393
  • Peer Reviewed
• [Presentation] Functional analysis of Dullard in ES cell differentiation, which isessential for germ cell formation in the gastrulating mouse embryo (2008)
  • Author(s): S. S. Tanaka, et.al.
  • Organizer: The 23^<rd> Naito Conference
  • Place of Presentation: 神奈川
  • Year and Date: 2008-11-12
• [Presentation] Functional analysis of Dullard in ES cell differentiation, which is essential for germ cell formation in the gastrulating mouse embryo (2008)
  • Author(s): Satomi S. Tanaka
  • Organizer: The 23^<rd> Naito Conference
  • Place of Presentation: 神奈川
  • Year and Date: 2008-11-12
• [Presentation] Dullard is essential for primordial germ cell formation and posterior extraembryonic mesoderm differentiation in the gastrulating mouse embryo (2008)
  • Author(s): S. S. Tanaka, et.al.
  • Organizer: 日本発生生物学会
  • Place of Presentation: 徳島
  • Year and Date: 2008-05-29
• [Presentation] Ifitm/mil/fragilis ファミリーによるマウス生殖細胞の移動の制御 (2008)
  • Author(s): 田中聡, 他
  • Organizer: 頭部形成研究会
  • Place of Presentation: 熊本
  • Year and Date: 2008-04-15
• [Presentation] Ifitm/mil/fragilisファミリーによるマウス生殖細胞の移動の制御 (2008)
  • Author(s): 田中聡
  • Organizer: 頭部形成研究会
  • Place of Presentation: 熊本
  • Year and Date: 2008-04-15
• [Presentation] Molecular mechanisms of the primordial germ cell allocation in the gastrulating mouse embryo (2007)
  • Author(s): S. S. Tanaka, et.al.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] Lhx1 activity is required for the localization of the primordial germ cells in the early mouse embryo (2007)
  • Author(s): S. S. Tanaka, et.al
  • Organizer: 日本発生生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2007-05-29