Mechanism for proximal-distal patterning in tail regeneration

• Project/Area Number: 19570210
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: University of Hyogo
• Principal Investigator: MOCHII Makoto University of Hyogo, 大学院・生命理学研究科, 准教授 (90202358)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: アフリカツメガエル / 尾部再生 / 基部・先端パターン / Wnt5a / 表皮細胞

Research Abstract

アフリカツメガエル幼生尾部の再生には、再生芽の先端で強く発現するXwnt-5aが重要な働きを持つ。Xwnt-5aの作用機構を明らかにすることを中心として、尾部再生における基部一先端パターン形成のメカニズムを明らかにしようとした。その結果、Xwnt-5は幼生尾部組織に直接作用すること。Canonical Wntシグナル経路ではなく、JNKシグナルを経由すること。再生芽の伸長やパターン形成にはWntシグナルに加えてFGFやhedgehogシグナルも重要であることなどがわかった。

Research Products

• [Journal Article] Xenopus Wnt-5a induces an ectopic larval tail at injured site, suggesting a crucial role for noncanonical Wnt signal in tail regeneration (2009)
  • Author(s): Sugiura, T, Tazaki, A, Ueno, N, Watanabe, K and Mochii, M
  • Journal Title: Mech. Dev. 126 Pages: 56-67
  • Peer Reviewed
• [Journal Article] Xenopus Wnt-5a induces an ectopic larval tail at injured site, suggesting a crucial role for noncanonical Wnt signal in tail regeneration. (2009)
  • Author(s): Sugiura, Takuji
  • Journal Title: Mech. Dev. 126 Pages: 56-67
  • Peer Reviewed
• [Journal Article] VegT, eFGF and Xbra cause overall posteriorization while Xwnt8 causes eye-level restricted posteriorization in synergy with chordin in early Xenopus development (2008)
  • Author(s): Fujii H, Sakai M, Nishimatsu S, Nohno T, Mochii M, Orii H, Watanabe K.
  • Journal Title: Dev Growth Differ 50 Pages: 169-180
  • NAID: 10021095838
  • Peer Reviewed
• [Journal Article] Spinal cord is required for proper regeneration of the tail in Xenopus tadpoles (2008)
  • Author(s): Taniguchi, Y, Sugiura, T, Tazaki, A, Watanabe, K, Mochii, M.
  • Journal Title: Dev Growth Differ 50 Pages: 109-120
  • NAID: 10021095639
  • Peer Reviewed
• [Journal Article] Spinal cord is required for proper regeneration of the tail in Xenopus tadpoles (2008)
  • Author(s): Taniguchi, Yuka
  • Journal Title: Dev. Growth Differ. 50 Pages: 109-120
  • Peer Reviewed
• [Journal Article] VegT, eFGF and Xbra cause overall posteriorization while Xwnt8 causes eye-level restricted posteriorization in synergy with chordin in early Xenopus development (2008)
  • Author(s): Hujii, Hidefumi
  • Journal Title: Dev. Growth Differ. 50 Pages: 169-180
  • Peer Reviewed
• [Journal Article] Tail regeneration in the Xenopus tadpole (2007)
  • Author(s): Mochii M, Taniguchi Y, Shikata I..
  • Journal Title: Dev Growth Differ 49 Pages: 155-161
  • NAID: 10018878701
  • Peer Reviewed
• [Journal Article] Tail regeneration in the Xenopus tadpole (2007)
  • Author(s): Mochii, Makoto
  • Journal Title: Dev. Growth Differ. 49 Pages: 155-161
  • NAID: 10018878701
  • Peer Reviewed
• [Presentation] Xenopus tadpole-derived XL-B4 myoblast cells transplanted into tadpole tails and limbs. (2008)
  • Author(s): Ishii, Takako
  • Organizer: 41st Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Tokushima
  • Year and Date: 2008-05-29
• [Presentation] In vitro migration of primoedial germ cell from Xenopus tailbud embryo. (2008)
  • Author(s): Terayama, Kohei
  • Organizer: 41st Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Tokushima
  • Year and Date: 2008-05-29
• [Presentation] The microRNA miR-427 controls PGC-specific expression of the DEADSouth mRNA in Xenopus (2008)
  • Author(s): Takeshi Yamaguchi, Kensuke Kataoka, Akira Tazaki, Hidefumi Orii, Makoto Mochii, Kenji Watanabe
  • Organizer: 12th International Xenopus Conference
  • Place of Presentation: Leiwen, Germany
• [Presentation] 生殖隆起に入った始原生殖細胞で起るXtrタンパク質の分解と新規合成 (2008)
  • Author(s): 杉本哲治, 片岡研介, 餅井真, 渡辺憲二, 高宗和史
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡
• [Presentation] Xenopus tadpole-derived XL-B4 myoblast cells transplanted into tadpole tails and limbs (2008)
  • Author(s): Takako Ishii, Shutaro Takayama, Kei Tamura, Makoto Mochii, Tadayoshi Shiba, Nobuhiko Takamatsu, Michihiko Ito
  • Organizer: 41st Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Tokushima
• [Presentation] Role of Notch signaling in PGC migration of Xenopus embryo (2008)
  • Author(s): Keisuke Morichika, Kensuke Kataoka, Kenji Watanabe, Makoto Mochii
  • Organizer: 41st Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Tokushima
• [Presentation] In vitro migration of primoedial germ cell from Xenopus tailbud embryo (2008)
  • Author(s): Kohei Terayama, Kensuke Kataoka, Keisuke Morichika, Hidefumi Orii, Kenji Watanabe, Makoto Mochii
  • Organizer: 41st Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Tokushima
• [Presentation] GFP-labeled mitochondria visualize germ plasm and primordial germ cells in Xenopus embryos (2008)
  • Author(s): Ayaka Taguchi, Miki Hirabayashi, Mochii Makoto, Watanabe Kenji
  • Organizer: 41st Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Tokushima
• [Presentation] Wnt5aは傷組織を末端化することによりXenopus幼生の尾部再生を誘導する (2007)
  • Author(s): 杉浦 太久至
  • Organizer: 日本動物学会大78回大会
  • Place of Presentation: 弘前大学
  • Year and Date: 2007-09-22
• [Presentation] アフリカツメガエル幼生尾部再生にはhedgehogシグナルが必須である (2007)
  • Author(s): 中畑 友梨子
  • Organizer: 日本動物学会大78回大会
  • Place of Presentation: 弘前大学
  • Year and Date: 2007-09-22
• [Presentation] Wnt-5aは傷組織を末端化することにより、Xenopus幼生の尾部再生を誘導する (2007)
  • Author(s): 杉浦太久至、田崎啓、上野直人、渡辺憲二、餅井真
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前
• [Presentation] ツメガエル卵母細胞の生殖質におけるミトコンドリアのATP合成酵素と超微細構造の変動 (2007)
  • Author(s): 向後直美、田崎啓、織井秀文、餅井真、渡辺憲二
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前
• [Presentation] 表皮に斑点状に発現するXenopuspolkadot遺伝子の単利と発現の解析 (2007)
  • Author(s): 吉井俊祐、山口雅裕、田崎啓、餅井真、木下勉
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前
• [Presentation] アフリカツメガエル幼生尾部再生にはhedgehogシグナルが必須である (2007)
  • Author(s): 中畑友梨子、谷口由華、杉浦太久至、渡辺憲二、餅井真
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前
• [Presentation] Dynamic change of a mitochondrial proteins during germ line development (2007)
  • Author(s): Naomi Kogo, Akira Tazaki, Hidefumi Orii, Makoto Mochii, Kenji Watanabe
  • Organizer: CDB Symposium 2007
  • Place of Presentation: Kobe
• [Presentation] Notch signal regulates PGC migration in the Xenopus embryo (2007)
  • Author(s): Keisuke Morichika, Kensuke Kataoka, Kenji Watanabe, Makoto Mochii
  • Organizer: CDB Symposium 2007
  • Place of Presentation: Kobe
• [Book] Electroporation in the regenerating tail of the Xenopus tadpole(Springer, Tokyo) (2009)
  • Author(s): Mochii, M., Taniguchi, Y.
  • Publisher: In Electroporation and Sonoporation in Developmental Biology(Ed. H. Nakamura)
• [Book] Electroporation in the regenerating tail of the Xenopus tadpole. In Electroporation and Sonoporation in Developmental Biology. (Ed. H. Nakamura) (2009)
  • Author(s): Mochii, Makoto
  • Publisher: Springer